Quantitative analysis of superoxide anion generation in living cells by using chemiluminescence video microscopy

Suzaki, Etsuko; Kawai, Eriko; Kodama, Yuka; Suzaki, Toshinobu; Masujima, Tsutomu

doi:10.1016/0304-4165(94)90058-2

Cited by 4 publications

(1 citation statement)

References 18 publications

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“…For example, several studies have demonstrated that GSTs exert a regulatory influence on the mitogen-activated protein kinase (MAPK) signaling pathway, especially when the MAPK signaling pathway takes part in cellular survival and death signaling caused by UV irradiation, H 2 O 2 treatment, and other stresses (Adler et al 1999;Yin et al 2000). Moreover, mtDNA mutations caused by ROS can enhance tumor cell metastasis, while GST expression is upregulated in a large variety of tumor types, as previously reported (Tang and Tu 1994;Mapp et al 2002; Segura-Aguilar and Paris 2014; Ishikawa et al 2008). GSTs are divided into three categories: cytosolic, microsomal, and mitochondrial; only two types of GSTs (cytosolic and microsomal) have been found in insects (Wang et al 2019).…”

Section: Introductionmentioning

confidence: 66%

Role of AccMGST1 in oxidative stress resistance in Apis cerana cerana

Zhao

Chao

Wáng

et al. 2019

Cell Stress and Chaperones

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As detoxification enzymes, proteins in the glutathione S-transferase (GST) superfamily are reported to participate in oxidative stress resistance. Nevertheless, microsomal GSTs (MGSTs), a unique subclass of the GST superfamily associated with membranes, are rarely studied in insects. Here, we isolated an MGST gene in Apis cerana cerana (AccMGST1) and verified its role in oxidative stress response. We found higher expression of AccMGST1 in protective or defensive tissue, that is, the epidermis, which indicated its role in stress resistance. Real-time quantitative PCR (qRT-PCR) analysis indicated that AccMGST1 was upregulated by oxidative stresses at the transcriptional level. In contrast, AccMGST1 expression was inhibited when the antioxidant vitamin C (VC) was fed to experimental bees. Through western blotting, we found that the protein level of AccMGST1 under oxidative stress corresponded to the transcript level. Disc diffusion and mixed-function oxidation (MFO) assays suggested that AccMGST1 can protect not only cells but also DNA against oxidative damage. Furthermore, we discovered that the expression patterns of known antioxidant genes were changed in A. cerana cerana after AccMGST1 was silenced by RNA interference (RNAi). Thus, we concluded that the gene AccMGST1 exerts a significant role in the antioxidant mechanism.

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