2013
DOI: 10.1002/0471140864.ps0311s73
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Quantitative Analysis of Surface Expression of Membrane Proteins Using Cold‐Adapted Proteases

Abstract: This unit presents an improved method for quantitative analysis of surface expression of membrane proteins utilizing a cold-adapted trypsin. Preservation of the proteolytic activity of the enzyme at 0° to 4°C allows cleavage of surface-expressed membrane proteins at temperatures at which trafficking of the mammalian plasmalemmal proteins is blocked. This provides an important advantage over established trypsin-cleavage protocols since it can be applied to membrane proteins with a fast turnover rate of the memb… Show more

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