Quantitative analysis of the expression of caspase 3 and caspase 9 in different types of atherosclerotic lesions in the human aorta

Mitochondrial DNA Damage in Atherosclerosis

“…The expression of the CASP9 gene did not practically change in all types of atherosclerotic lesions when compared to unaffected areas, except for fatty streaks, where the expression level was almost doubled (p = 0.084, p = 0.030, p = 0.002, and p = 0.046 when compared to the unaffected intima, initial lesions, lipofibrous plaque, and fibrous plaque, respectively). In the samples of fatty streaks and lipofibrous plaques, a significant correlation of the expression of the CASP9 gene was noted (r = 0.933, p = 0.031) [16]. It was found that the level of expression of the ENO1 gene increased in areas with severe atherosclerotic lesions (lipofibrous and fibrous plaques) at p = 0.021.…”

“…Their proportion increased in atherosclerotic lesions and accounted for about 20% of all cells. The percentage of cells expressing CD68, the antigen of macrophages, increased with the progression of atherosclerotic lesion: 3.9% (SD = 1.7), 6.1% (SD = 6.3), 13.2% (SD = 3.2), 13.1% (SD = 6.3), and 18.2% (SD = 8.8), respectively [16].…”

“…With double immunocytochemical staining, the CD68 antigen of macrophages was expressed not only in cells of hematogenic origin but also in some resident subendothelial cells. This suggests that the presence of CD68 antigen is not only a sign of monocytic origin of cells but also a marker of their phagocytic activity, which increases when atherosclerotic lesions are formed [16].…”

Molecular characterization of three caspases from Bostrychus sinensis and their transcriptional responses to bacteria and viruses

“…In mammals and teleosts, caspases are distributed in a wide range of tissues (Kumaresan et al, ; Sobenin et al, ; Sun, Ge, Zhu, Zhang, & Zhang, ). In Channa striatus , caspase‐1, caspase‐2, caspase‐3, caspase‐8 and caspase‐9 are highly expressed in immune organs of kidney and spleen (Kumaresan et al, ).…”

“…The greatest proximity of these sequences among the other fish species was observed with B. pectinirostris and P. flavescens that correspond to the Perciformes family. In mammals and teleosts, caspases are distributed in a wide range of tissues (Kumaresan et al, 2016;Sobenin et al, 2015;Sun, Ge, Zhu, Zhang, & Zhang, 2015). In Channa striatus, caspase-1, caspase-2, caspase-3, caspase-8 and caspase-9 are highly expressed in immune organs of kidney and spleen (Kumaresan et al, 2016).…”