Quantitative analysis of the seasonal and tissue-specific expression of Cry1Ab in transgenic maize Mon810

Nguyen, Hang Thu; Jehle, Johannes A.

doi:10.1007/bf03356208

Cited by 124 publications

(142 citation statements)

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“…In the case of maize, near-isogenic lines are widely considered the appropriate comparators, as they share the same genetic constitution except for the target gene/sequence, in this case, the MON810 cry1Ab gene expression cassette, and possibly other genes with genetic linkage to the target sequence (Zeven and Waninge, 1986). MON810 Bt-maize leaves are known to contain 5-to 20-fold higher Bt-toxin levels than maize kernels (Nguyen and Jehle, 2007), and to our knowledge, Bt-transgenic plant leaves have not previously been tested with the D. magna model. The hypothesis examined in this study was compared to its non-GM nearisogenic line (PAN 6Q-121), GM-maize hybrid PAN 6Q-321B (event MON810) does not exert any adverse effects on fitness parameters of D. magna during a full-life-cycle feeding experiment under chronic, high-dose dietary exposure to maize-leaf feed.…”

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confidence: 99%

Chronic Responses ofDaphnia magnaUnder Dietary Exposure to Leaves of a Transgenic (Event MON810) Bt–Maize Hybrid and its Conventional Near-Isoline

Holderbaum

Cuhra

Wickson

et al. 2015

Journal of Toxicology and Environmental Health, Part A

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Insect resistance is the second most common trait globally in cultivated genetically modified (GM) plants. Resistance is usually obtained by introducing into the plant's genome genes from the bacterium Bacillus thuringiensis (Bt) coding for insecticidal proteins (Cry proteins or toxins) that target insect pests. The aim of this study was to examine the hypothesis that a chronic, high-dose dietary exposure to leaves of a Bt-maize hybrid (GM event MON810, expressing a transgenic or recombinant Cry1Ab toxin), exerted no adverse effects on fitness parameters of the aquatic nontarget organism Daphnia magna (water flea) when compared to an identical control diet based on leaves of the non-GM near-isoline. Cry1Ab was immunologically detected and quantified in GM maize leaf material used for Daphnia feed. A 69-kD protein near Bt's active core-toxin size and a 34-kD protein were identified. The D. magna bioassay showed a resource allocation to production of resting eggs and early fecundity in D. magna fed GM maize, with adverse effects for body size and fecundity later in life. This is the first study to examine GM-plant leaf material in the D. magna model, and provides of negative fitness effects of a MON810 maize hybrid in a nontarget model organism under chronic, high dietary exposure. Based upon these results, it is postulated that the observed transgenic proteins exert a nontarget effect in D. magna and/or unintended changes were produced in the maize genome/metabolome by the transformation process, producing a nutritional difference between GM-maize and non-GM near-isoline.

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confidence: 99%

Chronic Responses ofDaphnia magnaUnder Dietary Exposure to Leaves of a Transgenic (Event MON810) Bt–Maize Hybrid and its Conventional Near-Isoline

Holderbaum

Cuhra

Wickson

et al. 2015

Journal of Toxicology and Environmental Health, Part A

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“…Leaf samples were chosen as reference samples because Cry1Ab toxin content is reportedly the highest in this plant tissue in MON 810 event Bt maize (Monsanto, 2002;Nguyen & Jehle, 2007;Székács et al, 2010a). The Cry1Ab-1Ac ELISA kit from Agdia is distributed as a qualitative test, allowing quantitative determination using user-supplied analytical standards.…”

Section: Resultsmentioning

confidence: 99%

“…ELISA methods are the method of choice for the analytical determination of Cry type lectins (Grothaus et al, 2006;Huber-Lukac, Luthy, & Braun, 1983;Walschus, Witt, & Wittmann, 2002;Wang et al, 2007), but other immunoanalytical formats, for example, dot blot (Tapp & Stotzky, 1995), lateral flow immunostrips (Ermolli et al, 2006a), microsphere-based immunoassay (Ermolli et al, 2006b;Fantozzi et al, 2007) or immunomagnetic electrochemical sensor (Volpe, Ammid, Moscone, Occhigrossi, & Palleschi, 2006) have also been reported. ELISAs are widely used for Cry toxin detection in microbial preparations (Crespo et al, 2008;Takahashi et al, 1998) and in GM plants or food (Adamczyk, Adam, & Hardee, 2001;Baumgarte & Tebbe, 2005;Bruns & Abel, 2003;Chen et al, 2009;Chilcutt & Tabashnik, 2004;Douville et al, 2005;Ezequiel, Reggiardo, Vallejos, & Permingeat, 2006;Harwood, Wallin, & Obrycki, 2005;Margarit, Reggiardo, Vallejos, & Permingeat, 2006;Mendelsohn, Kough, Zigfridais, & Matthewsm, 2003;Nguyen & Jehle, 2007;Sims & Berberich, 1996;Stave, 1999Stave, , 2002Xie & Shu, 2001;Zwahlen, Hilbeck, Gugerli, & Nentwig, 2003). Commercial ELISA kits are available and used frequently for detection of Cry toxins from B. thuringiensis or GM plants.…”

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confidence: 99%

“…A common problem, however, in reported Cry toxin concentrations in GM plants is the high variability among results from different laboratories, different cultivation sites, and sometimes even within the same GM plant variety at a single location (Then & Lorch, 2008). Moreover, Cry toxins are known to be produced in GM plants in a tissue-and time-specific manner (Nguyen & Jehle, 2007;Székács, Lauber, Juracsek, & Darvas, 2010a;Székács, Lauber, Takács, & Darvas, 2010b). Cry1Ab toxin content often scatter over wide ranges within a single survey, concerns regarding non-uniform expression levels of the transgene and plant-to-plant variation in Cry1Ab contents were raised (Nguyen & Jehle, 2007;Then & Lorch, 2008).…”

mentioning

confidence: 99%

“…Moreover, Cry toxins are known to be produced in GM plants in a tissue-and time-specific manner (Nguyen & Jehle, 2007;Székács, Lauber, Juracsek, & Darvas, 2010a;Székács, Lauber, Takács, & Darvas, 2010b). Cry1Ab toxin content often scatter over wide ranges within a single survey, concerns regarding non-uniform expression levels of the transgene and plant-to-plant variation in Cry1Ab contents were raised (Nguyen & Jehle, 2007;Then & Lorch, 2008). Therefore, it is of essential importance to develop validated quantification methods and protocols that can be used in different laboratories and yield reproducible, comparable results, at least to the degree possible.…”

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confidence: 99%

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Inter-laboratory comparison of Cry1Ab toxin quantification inMON 810maize by enzyme-immunoassay

Székacs

Weiss²,

Quist

et al. 2012

Food and Agricultural Immunology

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A laboratory ring trial was performed in four laboratories for determination of Cry1Ab toxin in leaf material of MON 810 maize using a standardised enzymelinked immunoassay protocol. Statistical analysis was carried out by the ISO 5725-2 guidelines, sample standard deviation and standard error, withinlaboratory and inter-laboratory SD and SE were calculated. Measured interlaboratory average values were 12.594.0, 15.394.6 and 72.6917.8 mg/g for three lyophilised samples, and 27.894.3 mg/g for a frozen sample, yet, Cry1Ab concentrations ranged 66.5Á160.1% of the corresponding IA. Determined concentrations by in-house protocols were statistically not different in one laboratory and different in two laboratories from the corresponding values by the joint protocol. Results emphasise the importance of a standardised protocol among laboratories for comparable quantitative Cry1Ab toxin determination. However, even when using a standardised protocol, significant differences still occur among toxin concentrations detected in different laboratories, although with a smaller range of variation.

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Evaluation of Chilo suppressalis resistance and analysis of CRY1C expression in transgenic rice

et al. 2022

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The insect resistance of Bacillus thuringiensis (Bt) transgenic rice (Oryza sativa L.) is mainly determined by the transcription of the CRY1C gene and the translation process of the Cry1C protein. With this in mind, we analyzed CRY1C expression and Cry1C protein content in a transgenic Bt insect‐resistant restorer line and its F1 hybrids, evaluated the resistance to Chilo suppressalis of various lines of rice, and determined the 50% lethal concentration (LC50) of Cry1C for C. suppressalis. In four transgenic rice lines, the relative expression of CRY1C was highest at the heading stage in most tissues. Among different tissues from the same developmental stage, CRY1C expression was highest in leaves, followed by stems and panicles. The relative expression of CRY1C was higher in the parent restorer line than in the F1 hybrids. The LC50 of the Cry1C protein for C. suppressalis was 4.016 μg g−1, and the Cry1C protein expression level of transgenic insect‐resistant rice exceeded this threshold at the heading stages and in stems. We next analyzed the lethality of each strain toward C. suppressalis. After 48 h, the mortality rate of second‐instar C. suppressalis larvae feeding on transgenic stem tissue was higher at the heading stage than at the tiller stage and varied from 66.7 to 91.7%. By documenting the temporal and spatial expression of CRY1C and evaluating C. suppressalis resistance to Bt transgenic rice, the risk of pests and diseases during the rice production process can be greatly reduced.

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Quantitative analysis of the seasonal and tissue-specific expression of Cry1Ab in transgenic maize Mon810

Cited by 124 publications

References 18 publications

Chronic Responses ofDaphnia magnaUnder Dietary Exposure to Leaves of a Transgenic (Event MON810) Bt–Maize Hybrid and its Conventional Near-Isoline

Chronic Responses ofDaphnia magnaUnder Dietary Exposure to Leaves of a Transgenic (Event MON810) Bt–Maize Hybrid and its Conventional Near-Isoline

Inter-laboratory comparison of Cry1Ab toxin quantification inMON 810maize by enzyme-immunoassay

Evaluation of Chilo suppressalis resistance and analysis of CRY1C expression in transgenic rice

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