Quantitative analysis of the sHLA‐G protein in seminal plasma

Schallmoser, Andreas; Raab, Monika; Karn, Thomas; Königsberger, Sebastian; Schmidt, E. T.; Breitenbach-Koller, Hannelore; Sänger, Nicole

doi:10.1111/aji.13152

Cited by 8 publications

(5 citation statements)

References 29 publications

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“…Schallmoser et al. reported no association of sHLA-G expression with female reproductive outcomes following IVF-ET ( 134 ). More evidence is needed to determine whether maternal circulating sHLA-G expression is a predictor for RIF and whether a combination of maternal-, paternal- and embryo-derived sHLA-G levels have more clinical significance than single detection.…”

Section: Discussionmentioning

confidence: 99%

Association of parental HLA-G polymorphisms with soluble HLA-G expressions and their roles on recurrent implantation failure: A systematic review and meta-analysis

Zeng

2022

Front. Immunol.

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IntroductionHLA-G plays a central role in immune tolerance at the maternal-fetal interface. The HLA-G gene is characterized by low allelic polymorphism and restricted tissue expression compared with classical HLA genes. HLA-G polymorphism is associated with HLA-G expression and linked to pregnancy complications. However, the association of parental HLA-G polymorphisms with soluble HLA-G (sHLA-G) expression and their roles in recurrent implantation failure (RIF) is unclear. The study aims to systematically review the association of HLA-G polymorphisms with RIF, the association of sHLA-G expression with RIF, and the association of HLA-G polymorphisms with sHLA-G expressions in patients attending in-vitro fertilization (IVF) treatment.MethodsStudies that evaluated the association of HLA-G polymorphisms with RIF, the association between sHLA-G expression with RIF, and the association between HLA-G polymorphisms with sHLA-G expressions in patients attending IVF treatment were included. Meta-analysis was performed by random-effect models. Sensitivity analysis was performed by excluding one study each time. Subgroup analysis was performed based on ethnicity.ResultsHLA-G 14bp ins variant is associated with a lower expression of sHLA-G in seminal or blood plasma of couples attending IVF treatment. The maternal HLA-G*010101 and paternal HLA-G*010102 alleles are associated with RIF risk compared to other alleles. However, single maternal HLA-G 14bp ins/del polymorphism, HLA-G -725 C>G/T polymorphism, or circulating sHLA-G concentration was not significantly associated with RIF in the general population. HLA-G 14bp ins/ins homozygous genotype or ins variant was associated with a higher risk of RIF in the Caucasian population.DiscussionSpecific HLA-G alleles or HLA-G polymorphisms are associated with sHLA-G expression in couples attending IVF treatment. Several HLA-G polymorphisms may be related to RIF, considering different ethnic backgrounds. A combined genetic effect should be considered in future studies to confirm the association of HLA-G polymorphisms and sHLA-G expressions in relation to RIF.

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Section: Discussionmentioning

confidence: 99%

Association of parental HLA-G polymorphisms with soluble HLA-G expressions and their roles on recurrent implantation failure: A systematic review and meta-analysis

Zeng

2022

Front. Immunol.

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“…After semen analysis, all seminal plasma samples were centrifuged at 300 g for 15 min in a conical 15 ml centrifuge tube. Then the supernatant was obtained and stored at −80°C (Schallmoser et al, ).…”

Section: Methodsmentioning

confidence: 99%

Association of seminal angiotensinogen with sperm motility and morphology in male infertility

et al. 2019

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Many researchers have shown that renin–angiotensin system (RAS) is involved in various important aspects of male reproduction. In this study, we assessed whether abnormal levels of seminal angiotensinogen (AGT) may be associated with semen parameters in infertile males. A total of 115 male patients were recruited, and semen parameters, seminal AGT and the electrolytes including K+, Na+, Cl−, P and Ca were evaluated. According to the World Health Organization (WHO) 2010 criteria, the patients were divided into two groups: G1 group with normal semen parameters (n = 42) and G2 group with subnormal semen parameters (n = 73). The level of seminal AGT was significantly higher in G2 group compared with G1 group. Moreover, the level of AGT was negatively correlated with the percentage of total motility (r = −.322, p = .000), progressive motility (PR) (r = −.339, p = .000) and morphologically normal forms (r = −.263, p = .004). This study suggests that elevated seminal AGT level is associated with increased risk of asthenospermia and teratozoospermia.

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“…Under normal conditions, the expression of HLA-G is restricted, mainly in placental trophoblast cells [ 7 ]. Interestingly, the HLA-G protein has also been found in the male reproductive system and seminal plasma [ 8 , 9 ]. Furthermore, HLA-G expression has been observed in other immune-privileged sites, such as the thymus [ 10 ], cornea [ 11 ], and pancreatic islets [ 12 ].…”

Section: The General Expression and Immune-suppressive Function Of Hl...mentioning

confidence: 99%

The Molecular Mechanisms of HLA-G Regulatory Function on Immune Cells during Early Pregnancy

Mao,

Feng,

Zhu

et al. 2023

Biomolecules

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Human leukocyte antigen-G (HLA-G) is a non-classical human major histocompatibility complex (MHC-I) molecule with the membrane-bound and soluble types. HLA-G is primarily expressed by extravillous cytotrophoblast cells located at the maternal–fetal interface during pregnancy and is essential in establishing immune tolerance. This review provides a comprehensive understanding of the multiple molecular mechanisms by which HLA-G regulates the immune function of NK cells. It highlights that HLA-G binds to microRNA to suppress NK cell cytotoxicity and stimulate the secretion of growth factors to support fetal growth. The interactions between HLA-G and NK cells also activate senescence signaling, promoting spiral artery remodeling and maintaining the balance of maternal–fetal immune responses. In addition, HLA-G can inhibit the function of decidual T cells, dendritic cells, and macrophages. Overall, the interaction between trophoblast cells and immune cells mediated by HLA-G plays a crucial role in understanding immune regulation at the maternal–fetal interface and offers insights into potential treatments for pregnancy-related diseases.

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Quantitative analysis of the sHLA‐G protein in seminal plasma

Cited by 8 publications

References 29 publications

Association of parental HLA-G polymorphisms with soluble HLA-G expressions and their roles on recurrent implantation failure: A systematic review and meta-analysis

Association of parental HLA-G polymorphisms with soluble HLA-G expressions and their roles on recurrent implantation failure: A systematic review and meta-analysis

Association of seminal angiotensinogen with sperm motility and morphology in male infertility

The Molecular Mechanisms of HLA-G Regulatory Function on Immune Cells during Early Pregnancy

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