Quantitative analysis of the tomato nuclear proteome during <i>Phytophthora capsici</i> infection unveils regulators of immunity

Howden, Andrew J. M.; Stam, Remco; Heredia, Victor Martinez; Motion, Graham B.; Have, Sara ten; Hodge, Kelly; Amaro, Tiago M. M. M.; Huitema, Edgar

doi:10.1111/nph.14540

Cited by 39 publications

(34 citation statements)

References 53 publications

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“…AtWRKY33 is also rapidly induced by the flg22 epitope as part of microbe-associated molecular pattern immunity, with downstream targets involved in ethylene and camalexin synthesis as well as other transcription factors and pathogen receptors [46]. Furthermore, in a proteomic study of P. capsici infection, the tomato WRKY33 homolog protein was found to be induced by 8 hpi and localized to the nucleus [47].…”

Section: A Reprogramming Of Gene Co-expression Network Of Infection mentioning

confidence: 99%

Developmentally regulated activation of defense allows for rapid inhibition of infection in age-related resistance to Phytophthora capsici in cucumber fruit

et al. 2020

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Background Age-related resistance (ARR) is a developmentally regulated phenomenon conferring resistance to pathogens or pests. Although ARR has been observed in several host-pathogen systems, the underlying mechanisms are largely uncharacterized. In cucumber, rapidly growing fruit are highly susceptible to Phytophthora capsici but become resistant as they complete exponential growth. We previously demonstrated that ARR is associated with the fruit peel and identified gene expression and metabolomic changes potentially functioning as preformed defenses. Results Here, we compare the response to infection in fruit at resistant and susceptible ages using microscopy, quantitative bioassays, and weighted gene co-expression analyses. We observed strong transcriptional changes unique to resistant aged fruit 2–4 h post inoculation (hpi). Microscopy and bioassays confirmed this early response, with evidence of pathogen death and infection failure as early as 4 hpi and cessation of pathogen growth by 8–10 hpi. Expression analyses identified candidate genes involved in conferring the rapid response including those encoding transcription factors, hormone signaling pathways, and defenses such as reactive oxygen species metabolism and phenylpropanoid biosynthesis. Conclusion The early pathogen death and rapid defense response in resistant-aged fruit provide insight into potential mechanisms for ARR, implicating both pre-formed biochemical defenses and developmentally regulated capacity for pathogen recognition as key factors shaping age-related resistance.

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Section: A Reprogramming Of Gene Co-expression Network Of Infection mentioning

confidence: 99%

Developmentally regulated activation of defense allows for rapid inhibition of infection in age-related resistance to Phytophthora capsici in cucumber fruit

et al. 2020

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“…capsici is a soil-borne pathogenic oomycete that causes severe blight and fruit rot of more than 50 plant species, including pepper, tomato, cucumber, and other commercially important crops (Lamour et al, 2012). Blight and fruit rot results in tremendous yield losses approximating $1 billion worldwide each year (Lamour et al, 2012;Howden et al, 2017). Recent research indicates that the P. capsica-Arabidopsis system is a model pathosystem for analyzing a wide range of oomycete-plant interactions (Wang et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

High-Throughput Sequencing-Based Identification of Arabidopsis miRNAs Induced by Phytophthora capsici Infection

Zhu

Fang

et al. 2020

Front. Microbiol.

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MicroRNAs (miRNAs) are a group of small non-coding endogenous RNAs. In plants, miRNAs play vital functions in regulating growth, development, and stress response. However, the role of miRNAs in Arabidopsis-Phytophthora capsici (P. capsici) model pathosystem is poorly understood. Here, we used a high-throughput sequencing approach to identify pathogen-responsive miRNAs using 15 small RNA (sRNA) libraries prepared from Arabidopsis thaliana leaves collected at 0, 3, 6, 12, and 24 h postinoculation with P. capsici. A total of 293 known miRNAs and 6 potential novel sRNAs (miRNAs or siRNAs) were identified, of which 33 miRNAs were differentially expressed at four different infection stages. To verify the reliability of the sRNA-seq results, we investigated the expression of five sRNAs upregulated throughout the four infection stages and their potential target genes using northern blot analysis and/or stem-loop quantitative real-time polymerase chain reaction (qRT-PCR). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses revealed that the potential target genes of the differentially expressed miRNAs, both conserved and novel, were enriched in pathways such as starch and sugar metabolism, spliceosome, and plant-pathogen interaction, indicating that the splicing machinery and pathogenesis-related (PR) proteins play important roles in the response to P. capsici infection. Taken together, these results provide novel insights into the molecular mechanisms of pathogenesis by P. capsici. Additionally, these results will serve as a strong foundation for further in-depth analysis of miRNAs involved in the resistance to Phytophthora species in other crops.

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“…AHL genes may also play a role in regulating homeostasis of gibberellins, jasmonic acid and cytokinins, and some AHL genes may be involved in regulating plant defence response (reviewed by Zhao et al, 160 ). The apparent orthologue of OG41488/FRAEX38873_v2_000363060.1 in S. lycopersicum is Solyc04g076220 and is also known as AHL17a 161 (Howden et al, 2017); the potential role of Solyc04g076220/AHL17a in immunity to a fungal pathogen in tomato has been investigated, but over-expression this gene did not visibly impact immunity 161 .…”

Section: The Serine Variant Inmentioning

confidence: 99%

Genes for ash tree resistance to an insect pest identified via comparative genomics

Plumb

Dw³

et al. 2019

Preprint

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Genome-wide discovery of candidate genes for functional traits within a species typically involves the sequencing of large samples of phenotyped individuals 1 , or linkage analysis through multiple generations 2 . When a trait occurs repeatedly among phylogenetically independent lineages within a genus, a more efficient approach may be to identify genes via detection of amino acid residues shared by species possessing that trait 3,4 . Here, by taking this approach, we identify candidate loci in the genus Fraxinus (ash trees) for resistance to the emerald ash borer beetle (EAB; Agrilus planipennis), a pest species that appears innocuous to otherwise healthy ash in its native East Asian range 5 but is highly destructive in North America 6 and poses a threat to ash trees in Europe 7 . Assembling whole genome sequences for 24 diploid species and subspecies of ash, and estimating resistance to EAB for 26 taxa from egg bioassays, we find 53 genes containing amino acid variants shared between two or more independent Fraxinus lineages with EABresistant species, that are unlikely to be due to chance or undetected paralogy. Of these, seven genes have putative roles relating to the phenylpropanoid biosynthesis pathway and 17 are potentially connected to herbivore recognition, defence signalling or programmed cell death. We also find that possible loss-of-function mutations among our 53 candidate genes are more frequent in susceptible species, 2 than in resistant ones. Patterns of polymorphism for the EAB-associated amino acid variants in ash trees representing different European populations suggest that selection may be able to enhance their resistance to EAB.

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Quantitative analysis of the tomato nuclear proteome during Phytophthora capsici infection unveils regulators of immunity

Cited by 39 publications

References 53 publications

Developmentally regulated activation of defense allows for rapid inhibition of infection in age-related resistance to Phytophthora capsici in cucumber fruit

Developmentally regulated activation of defense allows for rapid inhibition of infection in age-related resistance to Phytophthora capsici in cucumber fruit

High-Throughput Sequencing-Based Identification of Arabidopsis miRNAs Induced by Phytophthora capsici Infection

Genes for ash tree resistance to an insect pest identified via comparative genomics

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