Quantitative analysis of trimethylsilyl derivative of hydroxyurea in plasma by gas chromatography–mass spectrometry

James, Hutchinson; Nahavandi, Masoud; Wyche, Melville Q.; Taylor, Robert E.

doi:10.1016/j.jchromb.2005.11.033

Cited by 13 publications

(4 citation statements)

References 21 publications

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“…As its extraction from an aqueous or biological matrix is not so workable, the only way to prepare the sample for chromatography is to eliminate most the potentially interfering compounds, mainly proteins and lipids. The approach of James et al [20] who extracted HU in the organic solvent mixture contained in Toxi-Lab ® A tubes, did not produce feasible results in our hands, the extraction yield being less than 1%. Several deproteinizing reagents (perchloric acid 70 g/dL, trichloracetic acid 10 g/dL) and several solvents (ethanol, chloroform, hexane) in different proportions were tested.…”

Section: Sample Preparationcontrasting

confidence: 58%

See 1 more Smart Citation

Plasma hydroxyurea determined by gas chromatography–mass spectrometry

Kettani¹,

Cotton²,

Gulbis³

et al. 2009

Journal of Chromatography B

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Section: Sample Preparationcontrasting

confidence: 58%

“…Until now, gas chromatography-mass spectrometry (GC-MS) was only applied by James et al [20]. Claimed analytical performances are promising but, in spite of extensive trials, this method does not provide, according to our tests, useful results by reason of a very low extraction yield.…”

Section: Introductionmentioning

confidence: 81%

Plasma hydroxyurea determined by gas chromatography–mass spectrometry

Kettani¹,

Cotton²,

Gulbis³

et al. 2009

Journal of Chromatography B

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“…A number of analytical methods have been developed to quantify hydroxyurea in biological fluids, including spectrophotometric measurements by colorimetric techniques (Milks and Janes, 1956 ; Davidson and Winter, 1963 ; Bolton et al, 1965 ; Sivakumar et al, 2013 ; Legranda et al, 2017 ), electroanalytical determination (Naik et al, 2015 ), Nuclear Magnetic Resonance (NMR) (Main et al, 1987 ; Sorg et al, 2005 ; De Marco et al, 2011 ), High Performance Liquid Chromatography (HPLC) (Pujari et al, 1997 ; Iyamu et al, 1998 ; Manouilov et al, 1998 ), Gas Chromatography coupled to Mass Spectrometry (GC-MS) (James et al, 2006 ; Kettani et al, 2009 ; Garg et al, 2015 ), and Liquid chromatography—tandem mass spectrometry (LC-MS/MS) (Dalton et al, 2005 ; Usawanuwat et al, 2014 ; Marahatta et al, 2016 ; Hai et al, 2017 ). Despite the copious literature for HU detection, the assay of HU may be cumbersome due to its molecular dimension, reactivity and ability to chemical and enzymatic degradation (Iyamu et al, 1998 ; Marahatta and Ware, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

MicroNIR/Chemometrics Assessement of Occupational Exposure to Hydroxyurea

Risoluti

Materazzi

2018

Front. Chem.

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Portable Near Infrared spectroscopy (NIRs) coupled to chemometrics was investigated for the first time as a novel entirely on-site approach for occupational exposure monitoring in pharmaceutical field. Due to a significant increase in the number of patients receiving chemotherapy, the development of reliable, fast, and on-site analytical methods to assess the occupational exposure of workers in the manufacture of pharmaceutical products, has become more and more required. In this work, a fast, accurate, and sensitive detection of hydroxyurea, a cytotoxic antineoplastic agent commonly used in chemotherapy, was developed. Occupational exposure to antineoplastic agents was evaluated by collecting hydroxyurea on a membrane filter during routine drug manufacturing process. Spectra were acquired in the NIR region in reflectance mode by the means of a miniaturized NIR spectrometer coupled with chemometrics. This MicroNIR instrument is a very ultra-compact portable device with a particular geometry and optical resolution designed in such a manner that the reduction in size does not compromise the performances of the spectrometer. The developed method could detect up to 50 ng of hydroxyurea directly measured on the sampling filter membrane, irrespective of complexity and variability of the matrix; thus extending the applicability of miniaturized NIR instruments in pharmaceutical and biomedical analysis.

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“…Several methods have been developed to quantify hydroxyurea in biological samples: colorimetric techniques [10], liquid chromatography coupled with UV spectrophotometry [11,12], GC-MS [13,14], reversed phase liquid chromatography coupled with mass spectrometry [15]. Colorimetric methods require sample volumes of 250-500 µL and are insensitive.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Liquid Chromatography Method with Electrochemical Detection for Hydroxyurea Quantification in Human Plasma and Aqueous Solutions

Rodríguez¹,

Beukens²,

Debouge

et al. 2014

J Chromatogr Sep Tech

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Hydroxyurea is the unique drug having demonstrated a significant efficacy in sickle cell disease treatment. We developed a liquid chromatography method with electrochemical detection for hydroxyurea analysis in plasma and aqueous solutions. Analytical goals included an analytical range from 2 to 50 mg/L, a total imprecision lower than 15% and a total error lower than 30%. After protein precipitation with acetonitrile, the separation was performed on a C18 Atlantis T3 column and eluted with sodium acetate 25 mM, acetonitrile 2.5%, pH 6.5. Thioacetamide was used as internal standard. The method was linear for drug concentrations ranging from 0.5 to 50 mg/L and recovery was comprised between 100 and 120%. The intra-day precision was lower than 6.0% and between-day precision was lower than 11%. The detection limit was 0.18 and 0.63 mg/L for aqueous solution and plasma, respectively and the quantification limit was 1.0 and 1.2 mg/L for aqueous solution and plasma, respectively. No interference from urea was observed. The liquid chromatography method developed can be used for pharmacokinetic studies in plasma and other biological samples such as saliva or urine. It requires low sample volumes and a simple pre-treatment and it allows a direct measure of non-derivatized hydroxyurea.

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Quantitative analysis of trimethylsilyl derivative of hydroxyurea in plasma by gas chromatography–mass spectrometry

Cited by 13 publications

References 21 publications

Plasma hydroxyurea determined by gas chromatography–mass spectrometry

Plasma hydroxyurea determined by gas chromatography–mass spectrometry

MicroNIR/Chemometrics Assessement of Occupational Exposure to Hydroxyurea

Development and Validation of a Liquid Chromatography Method with Electrochemical Detection for Hydroxyurea Quantification in Human Plasma and Aqueous Solutions

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