Quantitative analysis of tropane alkaloids in biological materials by gas chromatography–mass spectrometry

Namera, Akira; Yashiki, Mikio; Hirose, Yasuo; Yamaji, Seiichi; Tani, Tohru; Kojima, Tohru

doi:10.1016/s0379-0738(02)00302-x

Cited by 68 publications

(39 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Some analytical methods have been developed for the determination of alkaloids in biological samples, such as gas chromatography (GC) [10], high-performance liquid chromatography (HPLC) [11], capillary electrophoresis (CE) [12,13], gas chromatography coupled with mass spectrometry (GC-MS) [14][15][16], and high-performance liquid chromatography coupled with (tandem) mass spectrometry (LC-MS, LC-MS/MS) [17][18][19][20][21][22][23]. In spite of the good separation capacity of GC or GC-MS, these two instruments were limited in analysis of most alkaloids of low volatility.…”

mentioning

confidence: 99%

Simultaneous Determination of Toxic Alkaloids in Blood and Urine by HPLC–ESI–MS/MS

Zhang

Huang

et al. 2012

Chromatographia

View full text Add to dashboard Cite

A sensitive and selective method for simultaneous determination of 29 toxic alkaloids in human blood and 31 in urine using high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry was developed and validated. The samples were diluted with 0.1 mol L -1 HCl, and the target alkaloids were purified by solid phase extraction. The separation of 31 alkaloids was carried out on a C18 column using a gradient mobile phase with 10 mmol L -1 ammonium formate in water with 0.1% formic acid and methanol at the rate of 0.25 mL min -1 . The triple-quadrupole mass spectrometer equipped with an electrospray source in the positive mode was set up in the dynamic multiple reactions monitoring mode (dynamic MRM) to detect the ion transitions of 31 alkaloids. The calibration curves were linear over a range of 0.5-400, 1-400, or 4-400 lg L -1 for target alkaloids in human blood and urine, and the correlation coefficients (r 2 ) was higher than 0.9943. The limit of determination and limit of quantification were 0.2-1 and 0.5-4 lg L -1 for blood and urine, respectively. The only exceptions were sanguinarine and chelerythrine in human blood. All the target alkaloids were stable under the test condition. In addition, the solvent effect and reconstituted solution were investigated. The method was validated and proved to be accurate and precise over the studied concentrations and suitable for poisoning diagnosis and forensic toxicology.

show abstract

mentioning

confidence: 99%

Simultaneous Determination of Toxic Alkaloids in Blood and Urine by HPLC–ESI–MS/MS

Zhang

Huang

et al. 2012

Chromatographia

View full text Add to dashboard Cite

show abstract

“…At present, high performance liquid chromatography (HPLC) with reversed phase columns are considered as method of choice (Friedman and Levin, 1989;Papadoyannis, 1995) and various individual methods have been described in detail (Paphassarang, 1985;He et al, 1989;, Papadoyannis et al 1993Kursinszki et al 2005, andMroczek et al 2006). HPLC methods have also been developed specifically for the analysis of tropane alkaloid in animal feeds , and for tropane alkaloids in biological samples such as serum and urine (Namera et al, 2002). In addition, radioimmunoassays are available for example for atropine in serum (Wurzburger et al, 1977).…”

Section: Methods Of Analysismentioning

confidence: 99%

Tropane alkaloids (from Datura sp.) as undesirable substances in animal feed - Scientific Opinion of the Panel on Contaminants in the Food Chain

2008

EFSA Journal

View full text Add to dashboard Cite

SUMMARYThe term tropane alkaloids refers to a group of more than 200 compounds best known for their occurrence in the family Solanaceae comprising over 100 genera and 3000 plant species. They have in common a two-ringed structure characterized by a pyrrolidine and a piperidine ring sharing a single nitrogen atom and two carbons atoms. The nitrogen atom at the end of the molecule, which characterizes the compounds as alkaloids, is in this group characteristically methylated. The most important natural tropane alkaloids are (-)-hyoscyamine and (-)-scopolamine (also known as hyoscine). High concentrations of these alkaloids have been found particularly in Datura stramonium and Datura ferox, as well as in Datura innoxia. The pattern of tropane alkaloids differs significantly and in Datura stramonium (also known as thorn apple or Jimson weed) hyoscyamine prevails in most parts of the plant, whereas in Datura ferox scopolamine is the major alkaloid Tropane alkaloids as undesirable substances in animal feedThe EFSA Journal (2008) 691, 2-55 produced. Datura plants are toxic for animals if ingested in larger amounts. Their seeds, which contain significant amounts of hyoscyamine and scopolamine, can be found as botanical impurities in feed materials, particularly in soybean and linseed products.Very little information on the actual contamination of feed materials is available, and previous reports on adverse health effects in animals refer in most cases to accidental intoxications following the consumption of Datura plants rather than to the contamination of linseed and sobean contaning feed materials. Hence no conclusive exposure assessment could be presented for farm animals.Overall, pigs have been shown to be among the most sensitive species to Datura poisoning. A worst case exposure estimate indicated that adverse pharmacological effects in pigs following exposure to Datura ferox seeds, mainly containing scopolamine, can not be entirely excluded at the current statutory limits of 3000 mg/kg feed. However, the limited data also suggested that it is not likely that the presence of Datura stramonium impurities in animals feed up to the current statutory level of 1000 mg/kg would present a risk to animal health.The mechanism of action of tropane alkaloids relates to their competitive antagonism at muscarinic acetylcholine receptors, preventing the binding of acetylcholine. According to the specificity and selectivity of muscarinergic acetylcholine receptors in different organs, the functions of smooth muscles and exocrine gland cells, as well as the heart rate, respiration and functions in the central nervous system are modulated. Certain tropanes such as atropine (the racemic mixture of (-)-and (+)hyoscyamine) as well as scopolamine (mainly as butylscopolamine bromide) are used in human and veterinary therapy for a variety of related indications. Reports of poisoning of livestock and experimental feeding studies describe as most common symptoms associated with tropane alkaloids exposure dryness of the mucosa in the uppe...

show abstract

“…The method used for the analysis of biological fluids and the relative evidential materials involved in this intoxication was based on routine protocols of the laboratory and the relative literature (7,8).…”

Section: Laboratory Analysismentioning

confidence: 99%

Accidental Poisoning after Ingestion of “Aphrodisiac” Berries: Diagnosis by Analytical Toxicology

Nikolaou

Papoutsis

Stefanidou

et al. 2012

The Journal of Emergency Medicine

View full text Add to dashboard Cite

Quantitative analysis of tropane alkaloids in biological materials by gas chromatography–mass spectrometry

Cited by 68 publications

References 12 publications

Simultaneous Determination of Toxic Alkaloids in Blood and Urine by HPLC–ESI–MS/MS

Simultaneous Determination of Toxic Alkaloids in Blood and Urine by HPLC–ESI–MS/MS

Tropane alkaloids (from Datura sp.) as undesirable substances in animal feed - Scientific Opinion of the Panel on Contaminants in the Food Chain

Accidental Poisoning after Ingestion of “Aphrodisiac” Berries: Diagnosis by Analytical Toxicology

Contact Info

Product

Resources

About