Quantitative analytical aspects of reversed-phase liquid chromatography with slurry-packed capillary columns

Borra, Claudio; Han, Soon M.; Novotný, Miloš V.

doi:10.1016/s0021-9673(01)94623-0

Cited by 60 publications

(42 citation statements)

References 21 publications

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“…If the calculation of the A, B, and C terms is camed out by using the Knox equation (4) values of 1.1, 2.1, and 0.13, respectively, are obtained. although the scarcity of data in the region of very low reduced velocities should be taken into account, the data agree with those reported for slurry packed columns [6] and with the values expected for well-packed columns [20]. If both the dry-packed columns are compared with analogous slurry-packed columns [6,11], it can be seen that similar values are obtained for the column performance (E,d and total porosity (.+.…”

Section: Resultssupporting

confidence: 84%

See 1 more Smart Citation

Liquid chromatography capillary columns dry‐packed with polar and nonpolar stationary phases

Crescentini

Mastrogiacomo

1991

J Microcolumn Separations

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Abstract. Fused silica capillary columns of 0.250-mm i.d., packed with 5-pm particles of nonpolar (octadecyl, octyl, and butyl) and polar (silica, diol, cyano, and amino) materials, have been prepared using a dry-packing technique. The performance of the columns has been evaluated using the reduced plate heightlreduced velocity equation and the separation impedance values. A comparison with similar slurry-packed columns shows that the dry-packing method may yield, in most cases, columns of analogous or better efficiency and requires a simpler apparatus and a shorter time for column preparation.

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Section: Resultssupporting

confidence: 84%

“…The original cell was replaced with a homemade 20-nL cell obtained by using a piece of 50-pm i.d. fused silica tubing where the polyimide coating was removed for the proper length [6]. The cell was connected to the column by means of a thick-wall Teflon tube.…”

Section: Methodsmentioning

confidence: 99%

Liquid chromatography capillary columns dry‐packed with polar and nonpolar stationary phases

Crescentini

Mastrogiacomo

1991

J Microcolumn Separations

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“…The question whether small-I.D. LC columns show better kinetic properties compared to their conventional counterparts has been the subject of much discussion in the literature [60][61][62][63][64][65]. The results of these investigations covering different column I.D.s, and performed under test conditions which were not always similar, can be summarized as follows: h values are typically in the same range as conventional LC columns.…”

Section: Column Characterizationmentioning

confidence: 99%

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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This review discusses many different aspects of microcolumn liquid chromatography (LC) and reflects the areas of microcolumn LC research interest over the past decades. A brief theoretical discussion on a number of major issues, such as column characterisation, chromatographic dilution effects and extracolumn band broadening in microcolumn LC is given. Recent progress in column technology and the demands and developments of instrumentation and accessories for microcolumn LC are also reviewed. Besides that, the developments in a large number of established and also more recent detection techniques for microcolumn LC are also discussed. The potential of hyphenation of microcolumn LC with other techniques, more particularly of multidimensional chromatography and microcolumn LC coupled to mass spectrometry is reviewed. Finally, the perspective of microcolumn LC separation methods is stressed by a number of relevant applications.

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“…Hoffmann and Blomberg prepared capillary columns with small reversed phase spherical particles (3 and 2 lm) and suggested that filtration, influenced by slurry density, is particularly important in the packing of 3 lm particles [37]. Borra and co-workers demonstrated a high degree of reproducibility with 250 lm ID capillary columns slurry packed with 5 lm Spherisorb particles [38]. Verzele and co-workers studied the stability of capillary columns (320 lm ID) and concluded that it is comparable to that of conventional stainless steel [41].…”

Section: Slurry Packing Of Capillary Columnsmentioning

confidence: 99%

Preparation and use of packed capillary columns in chromatographic and related techniques

Lanças¹,

Rodrígues

Freitas

2004

J of Separation Science

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In this paper general considerations related to the various approaches and parameters influencing the preparation of highly efficient and stable capillary columns for use in liquid chromatography and related techniques are presented and the column production process is discussed in some detail. The different packing methods available for delivering a packing material into a capillary column are discussed from a practical viewpoint. Packing with a gas (dry packing), packing with a liquid solvent or a mixture of solvents (slurry packing), packing with supercritical carbon dioxide, electrokinetic packing, and sol-gel packing technologies are introduced and discussed throughout the paper. Practical recommendations for obtaining highly efficient (high plate numbers) and stable capillary packed columns are also addressed and discussed.

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Quantitative analytical aspects of reversed-phase liquid chromatography with slurry-packed capillary columns

Cited by 60 publications

References 21 publications

Liquid chromatography capillary columns dry‐packed with polar and nonpolar stationary phases

Liquid chromatography capillary columns dry‐packed with polar and nonpolar stationary phases

Microcolumn liquid chromatography: instrumentation, detection and applications

Preparation and use of packed capillary columns in chromatographic and related techniques

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