Quantitative and Pattern Recognition Analyses for the Quality Evaluation of Magnoliae Flos by HPLC

Fang, Zhe; Shen, Chang Min; Moon, Dong Cheul; Son, Kun Ho; Son, Jong Keun; Woo, Mi Hee

doi:10.5012/bkcs.2010.31.11.3371

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…High‐performance liquid chromatography (HPLC) analysis/pattern recognition with multivariate statistical analysis can provide information about the chemical composition, including the marker compounds traditionally used for quality control. For this reason, several researchers have recently developed HPLC and pattern recognition analytical methods for the purpose of quality control and to determine the origin of certain herbal medicines . In our continued efforts to contribute to the chemical investigation of pharmaceutical plants, Raphani Semen (RS) was selected for further study.…”

Section: Introductionmentioning

confidence: 99%

Quantitative and Pattern Recognition Analyses of Five Marker Compounds in Raphani Semen using High‐Performance Liquid Chromatography

Jung

Lee

Zhao

et al. 2015

Bulletin Korean Chem Soc

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A rapid and simple high‐performance liquid chromatography (HPLC)‐photodiode array (PDA) analytical method was developed for the quantitative analysis of Raphani Semen (RS). This method was successfully used to determine the five main phenolic compounds found in RS specimens from different production regions. The compounds included sinapine thiocyanate (1), β‐d‐fructofuranosyl‐α‐d‐(6‐O‐sinapoyl)‐glucopyranoside (2), isorhamnetin 3,4′‐di‐O‐β‐d‐glucoside (3), β‐d‐(3‐O‐sinapoyl)‐fructofuranosyl‐α‐d‐(6‐O‐sinapoyl)‐glucopyranoside (4), and β‐d‐(3,4‐O‐disinapoyl)‐fructofuranosyl‐α‐d‐(6‐O‐sinapoyl)‐glucopyranoside (5). The marker compounds were separated using an Agilent Eclipse XDB‐C18 column (5.0 µm, 150 × 4.6 mm i.d.) by gradient elution with acetonitrile/water/0.1% trifluoroacetic acid (TFA) as the mobile phase (flow rate, 1.0 mL/min). This method was fully validated with respect to linearity, precision, accuracy, stability, and robustness. The HPLC analytical method was validated to conduct a pattern recognition analysis by repeatedly analyzing 56 seed samples including 55 RS (C01–C49 and K50–K55) and 1 Brassicae Semen samples. In addition, a content standard for RS was proposed. Compounds 1 and 4 were revealed as major components in the HPLC chromatogram, and their contents ranged from 0.06 to 0.20 and 0.02 to 0.35 mg/g, respectively. These results demonstrate the successful development of an analytical method suitable for evaluating the quality and distinguishing the origin of RS. In addition, we briefly describe the crucial liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) analytical conditions for the precise simultaneous quantification of the marker compounds.

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Section: Introductionmentioning

confidence: 99%

Quantitative and Pattern Recognition Analyses of Five Marker Compounds in Raphani Semen using High‐Performance Liquid Chromatography

Jung

Lee

Zhao

et al. 2015

Bulletin Korean Chem Soc

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“…Herbal magnoliae flos contains citral, eugenol, chavicol methyl ether, pinene and capric acid. The flowers are spicy and soft in nature, and are used as a stimulant in herbal medicine (Fang et al , 2010). In this paper, we isolated a novel strain from magnolia flowers.…”

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Paenibacillus kyungheensis sp. nov., isolated from flowers of magnolia

Ṣiddiqi

Kim

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

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A Gram-staining-positive, catalase-positive, oxidase-negative, facultatively anaerobic, rodshaped bacterium designated strain DCY88 T , was isolated from flowers of magnolia. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Paenibacillus that was closely related to Paenibacillus hordei RH-N24 T (97.8 %). The other most closely related species were Paenibacillus illinoisensis NRRL NRS-1356 T (94.3 %), Paenibacillus hunanensis DSM 22170 T (94.2 %), Paenibacillus peoriae DSM 8320 T (93.9 %), Paenibacillus kribbensis Am49 T (93.8 %) and the type species of the genus, Paenibacillus polymyxa ATCC 842 T (93.3 %). Cells of the strain were endospore-forming and motile by peritrichous flagella. Strain DCY88 T formed pinkpigmented colonies on trypticase soy agar and R2A agar medium. Growth of strain DCY88 T occurs at temperatures 5-37 8C, at pH 4-9 and 0.5-5.5 % NaCl (w/v). The menaquinone was MK-7.The cell wall peptidoglycan of strain DCY88 T contained meso-diaminopimelic acid. The major fatty acids were anteiso-C 15 : 0 (61.0 %) and C 16 : 0 (11.0 %). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified polar lipid. The strain DCY88 T contained spermidine as the major polyamine. The DNA G+C content was 51.6 mol%. The DNA-DNA hybridization relatedness between strain DCY88 T and P. hordei RH-N24 T was 48¡2 %. The phenotypic, phylogenetic and chemotaxonomic results indicate that the strain DCY88 T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus kyungheensis sp. nov. is proposed. The type strain is DCY88 T (5JCM 19886 T 5KCTC 33429 T ).The genus Paenibacillus, which belongs to the family Paenibacillaceae, is a group of bacilli that was described by Ash et al. (1993), and its description was emended by Shida et al. (1997a). Species of the genus Paenibacillus are either Gram-stain-positive or variable, facultatively anaerobic or strictly aerobic, produce ellipsoidal endospores, and are non-pigmented, rod-shaped and motile, with a DNA G+C content of 40 to 54 mol% (Ash et al., 1993; Shida et al., 1997a,b;Slepecky & Hemphill, 1992;Claus & Berkeley, 1986). Members of the genus are widespread microorganisms commonly isolated from various sources, including the rhizosphere of trees (Jiang et al., 2015;Jin et al., 2011b), c-irradiated Antarctic soil (Dsouza et al., 2014), duckweed (Kittiwongwattana & Thawai, 2015), nodules of Lupinus albus (Carro et al., 2014), tidal flats (Wang et al., 2012), water springs (Tang et al., 2011), rice seeds (Liu et al., 2010), iron mineral soil (Cao et al., 2015) and necrotic wounds (Glaeser et al., 2013). The cell wall peptidoglycan of members of the genus Paenibacillus is meso-diaminopimelic acid, and menaquinone-7 (MK-7) is the predominant menaquinone. Diphosphatidylglycerol is the major polar lipid in all the members of the genus Paenibacillus for which polar lipid data are available (Yao et al., 2014). The ...

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Analysis of lignans in Magnoliae Flos by turbulent flow chromatography with online solid‐phase extraction and high‐performance liquid chromatography with tandem mass spectrometry

Zhou

Chen

et al. 2016

J of Separation Science

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In this study, a method coupling turbulent flow chromatography with online solid-phase extraction and high-performance liquid chromatography with tandem mass spectrometry was developed for analyzing the lignans in Magnoliae Flos. By the online pretreatment of turbulent flow chromatography solid-phase extraction, the impurities removal and analytes concentration were automatically processed, and the lignans were separated rapidly and well. Seven lignans of Magnoliae Flos including epieudesmin, magnolin, 1-irioresinol-B-dimethyl ether, epi-magnolin, fargesin aschantin, and demethoxyaschantin were identified by comparing their retention behavior, UV spectra, and mass spectra with those of reference substances or literature data. The developed method was validated, and the good results showed that the method was not only automatic and rapid, but also accurate and reliable. The turbulent flow chromatography with online solid-phase extraction and high-performance liquid chromatography with tandem mass spectrometry method holds a high potential to become an effective method for the quality control of lignans in Magnoliae Flos and a useful tool for the analysis of other complex mixtures.

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Quantitative and Pattern Recognition Analyses for the Quality Evaluation of Magnoliae Flos by HPLC

Cited by 5 publications

References 16 publications

Quantitative and Pattern Recognition Analyses of Five Marker Compounds in Raphani Semen using High‐Performance Liquid Chromatography

Quantitative and Pattern Recognition Analyses of Five Marker Compounds in Raphani Semen using High‐Performance Liquid Chromatography

Paenibacillus kyungheensis sp. nov., isolated from flowers of magnolia

Analysis of lignans in Magnoliae Flos by turbulent flow chromatography with online solid‐phase extraction and high‐performance liquid chromatography with tandem mass spectrometry

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