Quantitative and qualitative analysis of Artemisiae Verlotori Folium and Artemisiae Argyi Folium by high‐performance liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

Abstract

Artemisiae Argyi Folium (Aiye in Chinese) has been widely used since ancient times. In the Lingnan region (Southern China), the leaf of Artemisia verlotorum Lamotte, which is named Hongjiaoai (HJA) because the roots are red (Hongjiao means red foot in Chinese), has been used as a local substitute for Artemisiae Argyi Folium. The plant has a long medicinal and edible history that can be traced to the Jin Dynasty. However, there is no systematic and reliable method to control the quality of Artemisiae Verlotori Folium. In this study, a comprehensive method involving high-performance liquid chromatography coupled with diode array detection and quadrupole-time-of-flight high-definition mass spectrometry was established to identify and quantify eight constituents (including organic acids and flavonoids) in Artemisiae Verlotori Folium and Artemisiae Argyi Folium as well as high-performance liquid chromatography fingerprints of the two varieties. Moreover, dissimilarities of chemical compositions among the two varieties were further investigated by orthogonal partial least squares discrimination analysis and cluster analysis. This research not only explored the similarities and differences between Artemisiae Verlotori Folium and Artemisiae Argyi Folium in eight components but also provided a qualitative and quantitative analytical method that quickly, accurately, and comprehensively assesses the quality of Artemisiae Verlotori Folium.Article Related Abbreviations: DAD, diode array detector; HCA, hierarchical clustering; HPLC-DAD-Q-TOF-MS, HPLC coupled with diode array detector and quadrupole-time-of-flight mass spectrometry; OPLS-DA, orthogonal partial least squares discriminant analysis; TCM, traditional Chinese medicine; VIP, variable importance in the projection.

Comparative Analysis of Active Ingredients and Potential Bioactivities of Essential Oils from Artemisia argyi and A. verlotorum

“…and Vaniot and A. verlotorum Lamotte were derived from different species of the same genus and have extremely similar morphological characteristics. These samples were (n = 3) collected from Nanyang, Henan Province, and Luofo mountain, Guangdong Province (specific medicinal plant cultivation sites) [62,63], People's Republic of China, respectively, and were collected strictly according to the medicinal age (1 year) and time of harvest. Both plants were identified by Prof. Chong-Ren Yang.…”

Analysis of cerebrospinal fluid metabolites affected by WenDanTang based on ultra‐high‐performance liquid chromatography coupled with high‐resolution mass spectrometry

“…Although we know many functions of the compounds in WDT, few can be identified in the cerebrospinal fluid (CSF). Ultra-high-performance liquid chromatography (UHPLC)-high-resolution mass spectrometry (HRMS) is an important tool for efficiently separating and identifying different components in fluid samples [13][14]. We obtained CSF metabolites affected by WDT using UHPLC-HRMS.…”

Elucidation of Anti-Obesity Mechanisms of Phenolics in Artemisiae argyi Folium (Aiye) by Integrating LC-MS, Network Pharmacology, and Molecular Docking

“…Artemisiae argyi Folium (Aiye) has been used for thousands of years as an herbal medicine to treat gynecological, skin, digestive, and respiratory diseases. Pharmacological research has shown that it has anti-virus, anti-bacteria, anti-oxidative, anti-inflammatory, anti-dermatitis, gastro-protective, and anti-cancer effects, mainly due to the presence of essential oils, flavonoids, and organic acids [ 12 , 13 , 14 , 15 , 16 , 17 , 18 ]. Pharmacological research has identified various anti-cancer components of Aiye, including jaceosidin, irigenin, luteolin, eriodictyol, apigenin, eupatilin, casticin, and artemetin [ 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ].…”