Quantitative and qualitative analysis of alkaloids composition in the seeds of a white lupin (Lupinus albus L.) collection

Kroc, Magdalena; Rybiński, W.; Wilczura, Paulina; Kamel, Katarzyna; Kaczmarek, Z.; Barzyk, Paweł; Świecicki, W.

doi:10.1007/s10722-016-0473-1

Cited by 40 publications

(46 citation statements)

References 8 publications

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“…Consequently, a limit of 0.02% of the seed dry weight has been assessed as a safe alkaloid content when used for food and feeding purposes [19]. Owing to the intensive breeding efforts, the seed alkaloid contents of modern lupin cultivars are often clearly lower than the accepted industry threshold [20,21].…”

Section: Introductionmentioning

confidence: 99%

Chromatographic Fingerprinting of the Old World Lupins Seed Alkaloids: A Supplemental Tool in Species Discrimination

Świecicki

Czepiel

Wilczura

et al. 2019

Plants

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The total contents and qualitative compositions of alkaloids in seeds of 10 Old World lupin species (73 accessions) were surveyed using gas chromatography. The obtained results, combined with those for three lupin crops, Lupinus angustifolius, Lupinus albus, and Lupinus luteus, provide the most complete and up-to-date overview of alkaloid profiles of 13 lupin species originating from the Mediterranean Basin. The qualitative alkaloid compositions served as useful supplementary tools of species discrimination. On the basis of the most abundant major alkaloids, lupanine, lupinine, and multiflorine, the Old World lupin species were divided into four groups. Those containing lupanine (L. angustifolius, L. albus, and Lupinus mariae-josephi), containing lupinine (Lupinus luteus, Lupinus hispanicus, and Lupinus × hispanicoluteus), containing lupinine and multiflorine (Lupinus atlanticus, Lupinus palaestinus, Lupinus anatolicus, Lupinus digitatus, Lupinus pilosus, and Lupinus cosentinii), and containing multiflorine (Lupinus micranthus). Within a given group, certain species can be, in most cases, further distinguished by the presence of other major alkaloids. The discrimination of species based on the total alkaloid content was found to be less reliable because of the significant intra-species variations, as well as the influences of environmental factors on the seed alkaloid content.

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Section: Introductionmentioning

confidence: 99%

Chromatographic Fingerprinting of the Old World Lupins Seed Alkaloids: A Supplemental Tool in Species Discrimination

Świecicki

Czepiel

Wilczura

et al. 2019

Plants

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“…The field experiments were performed in the experimental farm of the Poznań Plant Breeders Ltd. at Wiatrowo (Poland) using a procedure described by KROC et al (2017). In each season, replicated plots with three 1m-long rows spaced at 25 cm and 15 cm distance between plants were adopted.…”

Section: Materials and Methods Plant Materials And Experimental Designsmentioning

confidence: 99%

“…Useful features of the species are appreciated especially by farmers who are searching for alternative and/or further sources of protein, energy (oil, carbohydrates), lysine and vitamins in end products and/or by those who are advocating the rotational incomes of the crops in more sustainable production technologies (n-fixation by root nodule) (ABRAHAM et al, 2019;PETTERSON, 1998;BRUMMUND and ŚWIĘCICKI, 2011;ŚWIĘCICKI et al, 2007ŚWIĘCICKI et al, , 2015; GAWŁOWSKA and ŚWIĘCICKI, 2007). Although the Lupinus species are becoming progressively attractive for the European growers, enhanced concentrations and less acceptable properties of quinolizidine and piperidine alkaloids (ALKs) in the final products (seed meals and forage) appear to be the major antinutritional issues that limit their wider use (WINK, 2011;WINK et al, 1995;ANISZEWSKI, 2007;LEE et al, 2007;RESTA et al, 2008;BOSCHIN and RESTA, 2013;ANNICCHIARICO et al, 2014;KROC et al, 2017;FRICK et al, 2017). Hence, the strategy to breed lupin cultivars of a reduced concentration and/or rearranged composition of ALKs in seeds has become exceptionally important.…”

Section: Introductionmentioning

confidence: 99%

“…A broad genotypic variation in the seed accumulation of ALKs was already reported. According to KROC et al (2017) total alkaloid content for 367 accesions in L. albus varies from 0.02 to 12.73% of the seed dry weight. For wild lines and primitive populations (CO) the alkaloid content was between 0.06 and 12.73%, for present and old cultivars (CV) between 0.02 and 4.91%.…”

Section: Introductionmentioning

confidence: 99%

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Genetic analysis of alkaloid accumulation in seeds of white lupin (Lupinus albus L.)

Świecicki¹,

Gorny²,

Barzyk³

et al. 2019

Genetika

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2019): Genetic analysis of alkaloid accumulation in seeds of white lupin (Lupinus albus L.). Vol 51, No.3,[961][962][963][964][965][966][967][968][969][970][971][972][973] In the white lupin (Lupinus albus L.), the approach to breed 'sweet' cultivars with a reduced content of alkaloids in seeds and forage is challenging. The study was conducted to advance the limited knowledge on genetic causes controlling the accumulation/composition of alkaloids in seeds of the white lupin. Using a diallel cross design among the chosen 'sweet' and 'bitter' parents, all the range of genetic variation, inheritance mode, type of gene action, and combining abilities were examined among parents and their F1 and F2 progenies. Total alkaloid content and individual composition of alkaloids were evaluated by gas chromatography. In the study, a relatively complex mode of alkaloid inheritance was observed. For the proportion of some alkaloids (e.g. sparteine and ammodendrine), the simple additive-dominance model of analysis was less adequate suggesting an incidence of non-allelic interaction and dependent distribution of genes in parents. Both additive and non-additive gene effects were significant for most alkaloids, but the preponderance of gene dominance prevailed. Overdominance (mainly in F1) and partial or complete dominance (in F2) of genes was noticed for properties of most alkaloids. Directional dominance was evident and variable, depending on the examined alkaloids. Contribution of non-additive gene effects and the degree of dominance tended 962 GENETIKA, Vol. 51, No3, 961-973, 2019 to decrease among F2-progenies, suggesting a relatively rapid fixation of additivity in successive hybrid generations. However, selection for the content and composition of alkaloids should be more effective if the still lasting, masking non-additive gene causes would be eliminated in subsequent hybrid generations.

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“…Lupanine constitutes the major quinolizidine alkaloid present in the wastewater emitted from the manufacturing process of the L. albus L. snack [7,8]. The aforementioned alkaloid has attracted considerable attention in the biotechnological sector and it could be employed as feedstock for semi-synthesis of various novel high addedvalue compounds based on a series of useful functionalities of its asymmetric structure [9].…”

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confidence: 99%

Resolution of alkaloid racemate: a novel microbial approach for the production of enantiopure lupanine via industrial wastewater valorization

et al. 2020

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Background: Lupanine is a plant toxin contained in the wastewater of lupine bean processing industries, which could be used for semi-synthesis of various novel high added-value compounds. This paper introduces an environmental friendly process for microbial production of enantiopure lupanine.Results: Previously isolated P. putida LPK411, R. rhodochrous LPK211 and Rhodococcus sp. LPK311, holding the capacity to utilize lupanine as single carbon source, were employed as biocatalysts for resolution of racemic lupanine. All strains achieved high enantiomeric excess (ee) of L-(−)-lupanine (> 95%), while with the use of LPK411 53% of the initial racemate content was not removed. LPK411 fed with lupanine enantiomers as single substrates achieved 92% of D-(+)-lupanine biodegradation, whereas L-(−)-lupanine was not metabolized. Monitoring the transcriptional kinetics of the luh gene in cultures supplemented with the racemate as well as each of the enantiomers supported the enantioselectivity of LPK411 for D-(+)-lupanine biotransformation, while (trans)-6-oxooctahydro-1H-quinolizine-3-carboxylic acid was detected as final biodegradation product from D-(+)-lupanine use. Ecotoxicological assessment demonstrated that lupanine enantiomers were less toxic to A. fischeri compared to the racemate exhibiting synergistic interaction. Conclusions:The biological chiral separation process of lupanine presented here constitutes an eco-friendly and low-cost alternative to widely used chemical methods for chiral separation.

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Quantitative and qualitative analysis of alkaloids composition in the seeds of a white lupin (Lupinus albus L.) collection

Cited by 40 publications

References 8 publications

Chromatographic Fingerprinting of the Old World Lupins Seed Alkaloids: A Supplemental Tool in Species Discrimination

Chromatographic Fingerprinting of the Old World Lupins Seed Alkaloids: A Supplemental Tool in Species Discrimination

Genetic analysis of alkaloid accumulation in seeds of white lupin (Lupinus albus L.)

Resolution of alkaloid racemate: a novel microbial approach for the production of enantiopure lupanine via industrial wastewater valorization

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