Quantitative and qualitative analysis of pulmonary arterial hypertension fibrosis using wide-field second harmonic generation microscopy

Padrez, Yaraslau; Golubewa, Lena; Kulahava, Tatsiana; Vladimirskaja, T.E.; Семенкова, Г. Н.; Адзерихо, И. Э.; Yatsevich, Olga; Karpicz, Renata; Svirko, Yu. P.; Kuzhir, P.; Rutkauskas, Danielis

doi:10.1038/s41598-022-11473-5

Cited by 7 publications

(3 citation statements)

References 50 publications

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“…ML and DL algorithms trained on large datasets of annotated TPEM and H&E-stained images could identify patterns and features indicative of colorectal cancer, potentially even discovering new markers not previously recognized by human experts. Moreover, the integration of ML and DL approaches with high-throughput TPEM systems capable of generating images of entire histological slides [e.g., widefield SHG microscopy ( 41 , 42 ), and compact higher harmonic generation microscopy for unprocessed tissue ( 43 )] will enable direct comparisons between H&E-stained slides and their TPEM counterparts ( 44 ). This integration could simplify TPEM images interpretation, rendering it a promising avenue for further exploration.…”

Section: Discussionmentioning

confidence: 99%

Imaging of colorectal adenomas with pseudoinvasion and malignant polyps using two-photon excitation microscopy

Florea,

Eftimie,

Glogojeanu

et al. 2024

Front. Oncol.

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IntroductionAlthough the incidence and mortality rates of colorectal cancer exhibit significant variability, it remains one of the most prevalent cancers worldwide. Endeavors to prevent colorectal cancer development focus on detecting precursor lesions during colonoscopy. The diagnosis of endoscopically resected polyps relies on hematoxylin and eosin staining examination. For challenging cases like adenomatous polyps with epithelial misplacement, additional diagnostic methods could prove beneficial.MethodsThis paper aims to underscore stromal changes observed in malignant polyps and polyps with pseudoinvasion, leveraging two-photon excitation microscopy (TPEM), a technique extensively employed in the medical field in recent years. Results and discussionsBoth the subjective and quantitative analysis of TPEM images revealed distinct distributions and densities of collagen at the invasion front in malignant polyps compared to areas of pseudoinvasion. TPEM holds potential in discerning true invasion in malignant polyps from pseudoinvasion, offering enhanced visualization of local stromal changes.

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Section: Discussionmentioning

confidence: 99%

Imaging of colorectal adenomas with pseudoinvasion and malignant polyps using two-photon excitation microscopy

Florea,

Eftimie,

Glogojeanu

et al. 2024

Front. Oncol.

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“…Моделирование ЛАГ у 54 животных осуществляли с помощью МКТ [8,9]. МКТ растворяли в подогретом до 30 °С 0,9% растворе NaCl; 1 мл полученного раствора вводили крысам утром натощак в дозе 60 мг/кг массы тела подкожно в область холки.…”

Section: материал и методыunclassified

Analysis of the neutrophils role in the development of inflammation in monocrotaline-induced pulmonary arterial hypertension

Semenkova,

Adzerikho,

Amaegberi

et al. 2023

Lab Anim Sci Rus

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Pulmonary arterial hypertension (PAH) is a clinical syndrome with dismal outcome. This disease is associated with endothelial dysfunction or damage, pulmonary vascular remodeling, and increased vascular resistance and pulmonary artery blood pressure. All this provokes right ventricular failure and pulmonary edema, which leads to premature death of patients. PAH is accompanied by the inflammatory process development, one of the main causes of which is the increased production of reactive oxygen and chlorine species by stimulated neutrophils. The purpose of our study was to study the role of these cells in the oxidative stress formation in experimental model of PAH. The studies were conducted on outbred white rats. Modeling of PAH was carried out by subcutaneous administration of a monocrotaline solution at a dose of 60 mg/kg. The control group consisted of 10 healthy animals. After monocrotaline injection, PAH animals were divided into 4 groups: 10 rats in the 2-week group, 10 rats in the 4-week group, 14 rats in the 6-week group, and 20 rats in the 8-week group. After collecting peripheral blood and isolating neutrophils, reactive oxygen and chlorine species production, secretory degranulation and the unbound calcium ions concentration in the cytosol were determined using spectral analysis methods. The results were compared with corresponding data obtained for 10 healthy animals. It has been established that the development of PAH in rats over 8 weeks is accompanied by neutrophil priming and oxidative stress formation as a result of “respiratory burst” mechanisms modification. Changes in reactive oxygen and chlorine species production, disruption of Ca2+-dependent intracellular signaling involving cyclooxygenase 1/2 and phosphatidylinositol 3-kinase, and increased secretion of myeloperoxidase from neutrophils were revealed.

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“…To summarize, hemodynamic, histological and biochemical parameters determined in our study 3 weeks after MCT administration are characteristic for mild PH only. The most severe changes (including evident fibrosis) are observed mainly 4 weeks after MCT administration (Xu et al, 2018;Hołda et al, 2020a;Padrez et al, 2022).…”

Section: Induction Of Pulmonary Hypertensionmentioning

confidence: 99%

Effects of the peripheral CB1 receptor antagonist JD5037 in mono— and polytherapy with the AMPK activator metformin in a monocrotaline-induced rat model of pulmonary hypertension

et al. 2022

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Pulmonary hypertension (PH) is a disease leading to increased pressure in the pulmonary artery and right heart failure. The adenosine monophosphate-activated protein kinase (AMPK) activator, metformin, has a protective effect against PH. CB1 receptor blockade reduces the number of pathological alterations in experimental lung fibrosis. The current study evaluates the effect of the peripheral cannabinoid CB1 receptor antagonist JD5037 in mono- and polytherapy with metformin in rat monocrotaline-induced mild PH. Animals received metformin (100 mg/kg), JD5037 (3 mg/kg), or a combination of both once daily for 21 days. Monocrotaline (60 mg/kg) increased right ventricular (RV) systolic pressure (RVSP), led to RV and lung hypertrophy and remodeling, and decreased oxygen saturation. Metformin partially restored the monocrotaline-induced effects, i.e., decreased RVSP, increased oxygen saturation, and counteracted cardiac fibrotic, hypertrophic, and inflammatory changes. JD5037 modified parameters related to inflammation and/or fibrosis. Only polytherapy with metformin and JD5037 improved Fulton’s index and coronary artery hypertrophy and tended to be more effective than monotherapy against alterations in RVSP, oxygen saturation and coronary artery tunica media vacuolization. In conclusion, monotherapy with JD5037 does not markedly influence the PH-related changes. However, polytherapy with metformin tends to be more efficient than any of these compounds alone.

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Quantitative and qualitative analysis of pulmonary arterial hypertension fibrosis using wide-field second harmonic generation microscopy

Cited by 7 publications

References 50 publications

Imaging of colorectal adenomas with pseudoinvasion and malignant polyps using two-photon excitation microscopy

Imaging of colorectal adenomas with pseudoinvasion and malignant polyps using two-photon excitation microscopy

Analysis of the neutrophils role in the development of inflammation in monocrotaline-induced pulmonary arterial hypertension

Effects of the peripheral CB1 receptor antagonist JD5037 in mono— and polytherapy with the AMPK activator metformin in a monocrotaline-induced rat model of pulmonary hypertension

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