Quantitative and qualitative analysis of common peaks in chemical fingerprint of Yuanhu Zhitong tablet by HPLC-DAD–MS/MS

Abstract

A quality control (QC) strategy for quantitative and qualitative analysis of “common peaks” in chemical fingerprint was proposed to analyze Yuanhu Zhitong tablet (YZT), using high performance liquid chromatography with diode array detector and tandem mass spectrometry (HPLC-DAD–MS/MS). The chromatographic separation was achieved on an Agilent Eclipse plus C18 column with a gradient elution using a mixture of 0.4‰ ammonium acetate aqueous (pH 6.0 adjusted with glacial acetic acid) and acetonitrile. In chemical fingerprint, 40 peaks were assigned as the “common peaks”. For quantification of “common peaks”, the detection wavelength was set at 254 nm, 270 nm, 280 nm and 345 nm, respectively. The method was validated and good results were obtained to simultaneously determine 10 analytes (protopine, jatrorrhizine, coptisine, palmatine, berberine, xanthotoxin, bergapten, tetrahydropalmatine, imperatorin and isoimperatorin). For qualification of “common peaks”, 33 compounds including 10 quantitative analytes were identified or tentatively characterized using LC–MS/MS. These results demonstrated that the present approach may be a powerful and useful tool to tackle the complex quality issue of YZT.

Applications of High Performance Liquid Chromatography in the Analysis of Herbal Products

“…While probably because of low cost and simplicity, the use of HPLC-PDA seems to be the most common of all, many other sophisticated hyphenated techniques, e.g., LC-PDA-MS [21,67e69,74,76,77], are also used. For example, HPLC and LC-DAD-MS/MS were applied for the qualitative and quantitative analyses of Compound Kushen Injection, a well-known Chinese herbal product [74,78]. In that study, a simple fingerprinting approach was used; eight peaks were selected as the characteristic peaks in the HPLC chromatograms of 27 different batches of this herbal product.…”

“…Twelve batches of Yuanhu Zhitong tablets were obtained from various pharmaceutical companies in China [78]. Twelve batches of Yuanhu Zhitong tablets were obtained from various pharmaceutical companies in China [78].…”

“…A fully validated HPLC quantification and fingerprinting analysis [78] of each sample (5 mL) was performed on an Agilent 1260 series HPLC, utilizing an Agilent Eclipse plus C 18 column (250 Â 4.6 mm, 5 mm) with a binary gradient elution as follows: 0.4% ammonium acetate aqueous solution (pH adjusted to 6.0 with glacial acetic acid) as solvent A and acetonitrile as solvent B, initial 17% B, 0e25 min linear gradient 17e19% B, 25e55 min at 19% B, 55e70 min from 19 to 25% B, 70e80 min 25e28% B, 80e95 min 28e34% B, 95e120 min 34e35% B, 120e140 min 35e42% B and 140e160 min 42e50% B; flow rate 1 mL/min; column temperature at 30 C; quantitative detection wavelength at 254 nm (xanthotoxin, bergapten, imperatorin and isoimperatorin), 270 nm (berberine), 280 nm (protopine and tetrahydropalmatine) or 345 nm (jatrorrhizine, coptisine, and palmatine), while the wavelength of fingerprinting analysis was set at 280 nm. 7.…”

“…For quantification of "common peaks," the detection wavelength was set at 254, 270, 280, or 345 nm [78]. For quantification of "common peaks," the detection wavelength was set at 254, 270, 280, or 345 nm [78].…”

Intelligent Identification of Traditional Chinese Medicine Materials Based on Multi-feature Extraction and Pattern Recognition

“…Hence, objective methods seemed to be necessary, including microscope [1] , physical-chemical analysis [2,3] , mass-spectrum analysis [4] , and fingerprint [5,6] , machine olfaction (e-nose) [7][8][9][10] . However, methods mentioned in reference [1][2][3][4][5][6] were time-consuming and the operation of the instrument was complex; samples had to be destroyed and continuous observation to the same sample could not be carried on. For microscope and machine olfaction methods, expensive equipment were required, which was hard to be wild spread applied.…”