Quantitative and qualitative analysis of rat pup ultrasonic vocalization sounds induced by a hypothermic stimulus

Kromkhun, Pudcharaporn; Katou, Masahiro; Hashimoto, Haruo; Terada, Misao; Moon, Changjong; Saitô, Tôru

doi:10.5625/lar.2013.29.2.77

Cited by 24 publications

(25 citation statements)

References 14 publications

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“…The pitch of USVs from control pups decreased with age, as previously reported (Kromkhun et al, 2013), but increased with age in PS pups, further suggesting atypical development following PS. Isolation-induced USVs are inherently social (Hofer et al, 2002;Harmon et al, 2008), and reductions of USVs have been found in rodent models of ASDs (Crawley, 2007;Higashida et al, 2011).…”

Section: Discussionsupporting

confidence: 82%

Prenatal Stress Alters the Development of Socioemotional Behavior and Amygdala Neuron Excitability in Rats

Ehrlich

Rainnie

2015

Neuropsychopharmacol

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Prenatal stress (PS) is a risk factor for neurodevelopmental disorders with diverse ages of onset and socioemotional symptoms. Some PS-linked disorders involve characteristic social deficits, such as autism spectrum disorders and schizophrenia, but PS also promotes anxiety disorders. We propose the diversity of symptoms following PS arises from perturbations to early brain development. To this end, we characterized the effects of PS on the developmental trajectory of physiology of the amygdala, a late-developing center for socioemotional control. We found that PS dampened socioemotional behavior and reduced amygdala neuron excitability in offspring during infancy (at postnatal days (P)10, 14, 17 and 21), preadolescence (day 28), and adulthood (day 60). PS offspring in infancy produced fewer isolation-induced vocalizations and in adulthood exhibited less anxiety-like behavior and deficits in social interaction. PS neurons had a more hyperpolarized resting membrane potential from infancy to adulthood and produced fewer action potentials. Moreover, adult amygdala neurons from PS animals expressed larger action potential afterhyperpolarizations and H-current relative to controls, further limiting excitability. Our results suggest that PS can suppress socioemotional behavior throughout development and produce age-specific alterations to amygdala physiology.

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Section: Discussionsupporting

confidence: 82%

Prenatal Stress Alters the Development of Socioemotional Behavior and Amygdala Neuron Excitability in Rats

Ehrlich

Rainnie

2015

Neuropsychopharmacol

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“…As previously observed, we confirmed that the USVs produced by WT pups in response to a cool challenge changed as the pups matured (Kromkhun et al, 2013). Responses of WT pups to cool exposure significantly decrease as their fur growth and their ear canals open at 1 week of age.…”

Section: Resultssupporting

confidence: 90%

Intranasal oxytocin administration rescues neonatal thermo-sensory deficit in mouse model of Autism

Prato

Abdallah

Point

et al. 2019

Preprint

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Atypical responses to sensory stimuli are considered as a core aspect and early life marker of autism spectrum disorders (ASD). Although recent findings performed in mouse ASD genetic models report sensory deficits, these were explored exclusively during juvenile or adult period. Whether sensory dysfunctions might be present at the early life stage is unknown. Here we investigated cool thermosensibility during the first week of mouse life. In response to cool temperature exposure control neonates undertake innate behaviors by eliciting low-latency ultrasonic vocalization. However, we found that neonatal mice lacking the autism-associated gene Magel2 fail to react to cool stimuli while long-term thermoregulatory function, namely nonshivering thermogenesis, is active. Investigation of the sensory pathway revealed abnormal cool-induced response in the medial preoptic area.Importantly, intranasal administration of oxytocin can rescue this thermosensory reactivity. In addition, chemogenetic inactivation in control neonates of hypothalamic oxytocin neurons reproduces the coolness response failure observed in Magel2 mutants. Collectively, these findings establish for the first-time impairments of thermal lower-reactivity in a mouse model of ASD with deletion of Magel2 gene during early life period and reveal that the oxytocinergic system regulates this neonatal cool thermosensibility.

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“…Previous studies have elucidated the developmental changes in the acoustic characteristics of USV emitted by rat pups upon maternal isolation (Kehoe & Harris, 1989;Elsner et al, 1990;Brudzynski et al, 1999;Schwarting & Wohr, 2012;Kromkhun et al, 2013;Wada, 2017) and reported that the number of USVs increases on PNDs 3-5 to reach a…”

Section: Discussionmentioning

confidence: 99%

“…High-ethanol male Low-ethanol male Control male High-ethanol female Low-ethanol female Control female peak on PNDs 5-11 (Brudzynski et al, 1999;Schwarting & Wohr, 2012). USV duration exhibits an age-dependent increase, whereas USV frequency exhibits an age-dependent decrease (Elsner et al, 1990;Kromkhun et al, 2013). Subsequently, as they age, the pups become less dependent on their dam because they are covered with body hair and can regulate their body temperature.…”

Section: Rms Of Usv Amplitude (V)mentioning

confidence: 99%

Effects of prenatal ethanol exposure on acoustic characteristics of ultrasonic vocalizations in rat pups

Shahrier

Wada

2018

NeuroToxicology

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Rat pups produce ultrasonic vocalizations (USVs) on isolation from their dam. Ultrasonic vocalization is a sensitive tool for evaluating social behavior between pups and their dam. Prenatal ethanol-exposure leads to a reduction in USVs and have the potential of inducing difficulties in social behavior between pups and their dam. However, effects of prenatal ethanol-exposure on the acoustic characteristics of USVs remain unclear. In this study, we recorded USVs produced by rat pups that were prenatally exposed to ethanol and examined their acoustic characteristics. Ethanol was administered to 13 pregnant rats in three stages by gradually increasing concentrations between gestational days (GDs) 8-20. From GDs 14-20, ethanol-containing tap water at concentrations of 30% and 15% (v/v) was administered to the high- and low-ethanol groups, respectively. Tap water without ethanol was given to the control group. On postnatal days (PNDs) 4, 8, 12, and 16, individual newly-born pups were isolated from their dam and littermates and USVs produced by them were recorded for 5 min. The number of USVs in the high-ethanol group was greater than that in both low-ethanol and control groups on PND 12. The mean, minimum, and maximum fundamental frequencies of USVs were elevated in the high-ethanol group compared with that in both low-ethanol and control groups. Higher amplitudes of USVs were produced by male pups in the high-ethanol group than in those in both low-ethanol and control groups on PND 12. These results suggest that prenatal ethanol exposure changed emotionality and accordingly, the high-ethanol group produced more USVs as distress calls.

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Quantitative and qualitative analysis of rat pup ultrasonic vocalization sounds induced by a hypothermic stimulus

Cited by 24 publications

References 14 publications

Prenatal Stress Alters the Development of Socioemotional Behavior and Amygdala Neuron Excitability in Rats

Prenatal Stress Alters the Development of Socioemotional Behavior and Amygdala Neuron Excitability in Rats

Intranasal oxytocin administration rescues neonatal thermo-sensory deficit in mouse model of Autism

Effects of prenatal ethanol exposure on acoustic characteristics of ultrasonic vocalizations in rat pups

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