Quantitative and qualitative changes in AMPA receptor expression during spinal cord development

Jakowec, Michael W.; Fox, Albert J.; Martin, Lee J.; Kalb, Robert G.

doi:10.1016/0306-4522(95)00026-f

Cited by 130 publications

(72 citation statements)

References 44 publications

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“…The details of immunoblot procedures and quantitative analysis are described in the supplemental material (available at www.jneurosci.org). Immunohistological techniques have been described previously (Jakowec et al, 1995;Stegenga et al, 2003).…”

Section: Methodsmentioning

confidence: 99%

Expression of Serum- and Glucocorticoid-Inducible Kinase Is Regulated in an Experience-Dependent Manner and Can Cause Dendrite Growth

David¹,

Stegenga²,

Hu³

et al. 2005

J. Neurosci.

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The interaction of an animal with its environment during a critical period in early postnatal life has lifelong effects on the structure and function of sensory and motor systems. To gain insight into the molecular mechanisms of experience-dependent development, we challenged young rats to adapt to a new environment that engenders novel motor behavior. Rats born in the gravitational field (1G) of the earth subsequently were reared for 2 weeks either in the absence of gravity (microgravity) or at 1G. A comparison of gene expression using microarrays led to the identification of a panel of differentially regulated transcripts. We report here that the abundance of serum-and glucocorticoid-inducible kinase (SGK) is increased in spinal cord tissue from animals reared in microgravity in comparison with 1G-reared controls. The induction of SGK expression also can be achieved by administration of glucocorticoids to animals at 1G or neurons in vitro. Expression of constitutively active SGK in neurons leads to the elaboration of neuronal dendrites and their branching. Glucocorticoids also lead to dendrite elaboration, and this effect can be abrogated by inhibiting SGK activity. Changes in the level of expression of SGK could be part of the mechanism for experience-dependent acquisition of mature neuronal properties.

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Section: Methodsmentioning

confidence: 99%

Expression of Serum- and Glucocorticoid-Inducible Kinase Is Regulated in an Experience-Dependent Manner and Can Cause Dendrite Growth

David¹,

Stegenga²,

Hu³

et al. 2005

J. Neurosci.

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“…The remaining differences in the KA/Glu ratios may have been because of a Q/R site-dependent modulation of the kainate efficacy or could have been caused by a reportedly incomplete (24) CTZ-mediated abolishment of desensitization in flop isoforms. GluR1-flop was used in this study because it is the splice variant presumably expressed in the adult brain (25). Evidence that kainate efficacy is also affected by the interaction of stargazin with the receptor has been reported previously (17).…”

Section: Stargazin Modulates the Extent Of Desensitization And The Kamentioning

confidence: 99%

Stargazin Interaction with α-Amino-3-hydroxy-5-methyl-4-isoxazole Propionate (AMPA) Receptors Is Critically Dependent on the Amino Acid at the Narrow Constriction of the Ion Channel

Körber

Werner

Hoffmann

et al. 2007

Journal of Biological Chemistry

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The subunit GluR2 of the ␣-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) subfamily of ionotropic glutamate receptors (GluRs) features a single amino acid at the narrow constriction of the pore loop that is altered from glutamine to arginine by RNA editing. This so-called Q/R site has been shown to play an important role in the determination of the electrophysiological properties of AMPA receptor complexes as well as of trafficking to the plasma membrane. The protein stargazin has also been shown to modulate electrophysiological properties and trafficking to the plasma membrane of AMPA receptors. In this study we examined via a series of mutants of the Q/R site of the AMPA receptor GluR1 whether the amino acid at this position has any influence on the modulatory effects mediated by stargazin. To this end, we analyzed current responses of Q/R site mutants upon application of glutamate and kainate and determined the amount of mutant receptor protein in the plasma membrane in Xenopus oocytes. Desensitization kinetics of several mutants were analyzed in HEK293 cells. We found that the stargazin-mediated decrease in receptor desensitization, the slowing of desensitization kinetics, and the kainate efficacy were all dependent on the amino acid at the Q/R site, whereas the stargazin-mediated increase in trafficking toward the plasma membrane remained independent of this amino acid. We propose that the Q/R site modulates the interaction of stargazin with the transmembrane domains of AMPA receptors via an allosteric mechanism and that this modulation leads to the observed differences in the electrophysiological properties of the receptor.

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“…Activity-dependent restructuring of neuronal circuits has primarily been examined for thalamic and cortical sensory maps, but evidence for its role in motoneuron development is growing (361, 487,599,750,899,900). Developmental alterations in motoneuronal glutamate receptor subunit expression (558,559), binding profiles (432,595,601), changing contributions of receptor subtypes to synaptic transmission (719,1433), and reductions in NMDA-induced currents (516, 876) underscore not only the potential for developmental change in motoneuron excitability but the possible contribution of glutamate receptors to activity-dependent development of motoneurons.…”

Section: Role Of Glutamate In Activity-dependent Development Of Motonmentioning

confidence: 99%

Synaptic Control of Motoneuronal Excitability

Rekling

Funk

Bayliss

et al. 2000

Physiological Reviews

537

482

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Movement, the fundamental component of behavior and the principal extrinsic action of the brain, is produced when skeletal muscles contract and relax in response to patterns of action potentials generated by motoneurons. The processes that determine the firing behavior of motoneurons are therefore important in understanding the transformation of neural activity to motor behavior. Here, we review recent studies on the control of motoneuronal excitability, focusing on synaptic and cellular properties. We first present a background description of motoneurons: their development, anatomical organization, and membrane properties, both passive and active. We then describe the general anatomical organization of synaptic input to motoneurons, followed by a description of the major transmitter systems that affect motoneuronal excitability, including ligands, receptor distribution, pre-and postsynaptic actions, signal transduction, and functional role. Glutamate is the main excitatory, and GABA and glycine are the main inhibitory transmitters acting through ionotropic receptors. These amino acids signal the principal motor commands from peripheral, spinal, and supraspinal structures. Amines, such as serotonin and norepinephrine, and neuropeptides, as well as the glutamate and GABA acting at metabotropic receptors, modulate motoneuronal excitability through pre-and postsynaptic actions. Acting principally via second messenger systems, their actions converge on common effectors, e.g., leak K + current, cationic inward current, hyperpolarization-activated inward current, Ca 2+ channels, or presynaptic release processes. Together, these numerous inputs mediate and modify incoming motor commands, ultimately generating the coordinated firing patterns that underlie muscle contractions during motor behavior.

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Quantitative and qualitative changes in AMPA receptor expression during spinal cord development

Cited by 130 publications

References 44 publications

Expression of Serum- and Glucocorticoid-Inducible Kinase Is Regulated in an Experience-Dependent Manner and Can Cause Dendrite Growth

Expression of Serum- and Glucocorticoid-Inducible Kinase Is Regulated in an Experience-Dependent Manner and Can Cause Dendrite Growth

Stargazin Interaction with α-Amino-3-hydroxy-5-methyl-4-isoxazole Propionate (AMPA) Receptors Is Critically Dependent on the Amino Acid at the Narrow Constriction of the Ion Channel

Synaptic Control of Motoneuronal Excitability

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