Quantitative and Qualitative Changes in the Genetic Diversity of Bacterial Communities in Anaerobic Bioreactors with the Diatomaceous Earth/Peat Cell Carrier

Pilarska, Agnieszka A.; Wolna-Maruwka, Agnieszka; Niewiadomska, Alicja; Grządziel, Jarosław; Gałązka, Anna; Paluch, Emil; Borowiak, Klaudia; Pilarski, Krzysztof

doi:10.3390/cells11162571

Cited by 4 publications

(19 citation statements)

References 71 publications

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“…A slightly lower level of 0.95064 was noted for WFC + S/L, while the lowest level was achieved by the control systems with the addition of PLAP, particularly without co-substrate (0.7467 for WF + PLAP). High biomass conversion rates depend on many factors, including, above all, the quantitative and qualitative composition of the digestion mixture, the presence of inhibitors, parameters of the process, and the condition of the bacterial microbiome [7,60]. It is interesting to note that the addition of granulated PLA had a slightly more favourable impact on improving the efficiency of the AD process in the systems discussed than the carrier materials researched by Pilarska et al earlier [7,39,53].…”

Section: Anaerobic Digestion Stability and Performancementioning

confidence: 92%

“…High biomass conversion rates depend on many factors, including, above all, the quantitative and qualitative composition of the digestion mixture, the presence of inhibitors, parameters of the process, and the condition of the bacterial microbiome [7,60]. It is interesting to note that the addition of granulated PLA had a slightly more favourable impact on improving the efficiency of the AD process in the systems discussed than the carrier materials researched by Pilarska et al earlier [7,39,53]. In principle, the effect of PLAG is comparable to the silica/lignin system (S/L; 4:1), [53] while the introduction of powdered PLA resulted in the lowest efficiency among the tested carriers.…”

Section: Anaerobic Digestion Stability and Performancementioning

confidence: 99%

Section: Anaerobic Digestion Stability and Performancementioning

confidence: 99%

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Polylactide (PLA) as a Cell Carrier in Mesophilic Anaerobic Digestion—A New Strategy in the Management of PLA

et al. 2022

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The management of waste polylactide (PLA) in various solutions of thermophilic anaerobic digestion (AD) is problematic and often uneconomical. This paper proposes a different approach to the use of PLA in mesophilic AD, used more commonly on the industrial scale, which consists of assigning the function of a microbial carrier to the biopolymer. The study involved the testing of waste wafers and waste wafers and cheese in a co-substrate system, combined with digested sewage sludge. The experiment was conducted on a laboratory scale, in a batch bioreactor mode. They were used as test samples and as samples with the addition of a carrier: WF—control and WFC—control; WF + PLA and WFC + PLA. The main objective of the study was to verify the impact of PLA in the granular (PLAG) and powder (PLAP) forms on the stability and efficiency of the process. The results of the analysis of physicochemical properties of the carriers, including the critical thermal analysis by differential scanning calorimetry (DSC), as well as the amount of cellular biomass of Bacillus amyloliquefaciens obtained in a culture with the addition of the tested PLAG and PLAP, confirmed that PLA can be an effective cell carrier in mesophilic AD. The addition of PLAG produced better results for bacterial proliferation than the addition of powdered PLA. The highest level of dehydrogenase activity was maintained in the WFC + PLAG system. An increase in the volume of the methane produced for the samples digested with the PLA granules carrier was registered in the study. It went up by c.a. 26% for WF, from 356.11 m3 Mg−1 VS (WF—control) to 448.84 m3 Mg−1 VS (WF + PLAG), and for WFC, from 413.46 m3 Mg−1 VS, (WFC—control) to 519.98 m3 Mg−1 VS (WFC + PLAG).

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Section: Anaerobic Digestion Stability and Performancementioning

confidence: 92%

Section: Anaerobic Digestion Stability and Performancementioning

confidence: 99%

Section: Anaerobic Digestion Stability and Performancementioning

confidence: 99%

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Polylactide (PLA) as a Cell Carrier in Mesophilic Anaerobic Digestion—A New Strategy in the Management of PLA

et al. 2022

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“…The addition of PLA appears to have no effect on the growth of Syntrophomonas . This is different to the changes occurring in systems with DEP and with silica/lignin materials, in which bacteria of the genus Syntrophomonas predominated in the systems of confectionery waste digested with the aforementioned carriers [ 34 , 39 ]. There are many reports that have investigated the role of Syntrophomonas in anaerobic digestion and the importance of bacteria belonging to the Syntrophomonadaceae family during the methane production from LCFA (long chain fatty acid) and called this a syntrophic partnership with methanogens [ 39 , 41 ] The methane production from LCFA occurs through a syntrophic partnership between LCFA β-oxidising acetogenic bacteria and methanogenic archaea that are involved in maintaining low concentrations of acetate and hydrogen.…”

Section: Resultsmentioning

confidence: 97%

“…The addition of PLA enhances cell proliferation and activity up to the last period of the AD process, resulting in improved biodegradability of the matter and its higher methane production efficiency, as reported in the earlier study carried out by the authors [ 17 ]. However, it must be presumed that the addition of this polymer does not act as selectively as DEP [ 39 ]—the amount of unique taxa in the CW + PLA sample remained comparable to the CW–control ( Figure 9 ). Furthermore, the results of the statistical analysis revealed no statistically significant differences ( Table 2 and Table 3 ) between the three systems tested.…”

Section: Resultsmentioning

confidence: 99%

Biofilm Formation and Genetic Diversity of Microbial Communities in Anaerobic Batch Reactor with Polylactide (PLA) Addition

Pilarska,

Marzec-Grządziel,

Paluch

et al. 2023

IJMS

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In this paper, an anaerobic digestion (AD) study was conducted on confectionery waste with granular polylactide (PLA) as a cell carrier. Digested sewage sludge (SS) served as the inoculum and buffering agent of systems. This article shows the results of the analyses of the key experimental properties of PLA, i.e., morphological characteristics of the microstructure, chemical composition and thermal stability of the biopolymer. The evaluation of quantitative and qualitative changes in the genetic diversity of bacterial communities, performed using the state-of-the-art next generation sequencing (NGS) technique, revealed that the material significantly enhanced bacterial proliferation; however, it does not change microbiome biodiversity, as also confirmed via statistical analysis. More intense microbial proliferation (compared to the control sample, without PLA and not digested, CW–control, CW–confectionery waste) may be indicative of the dual role of the biopolymer—support and medium. Actinobacteria (34.87%) were the most abundant cluster in the CW–control, while the most dominant cluster in digested samples was firmicutes: in the sample without the addition of the carrier (CW–dig.) it was 68.27%, and in the sample with the addition of the carrier (CW + PLA) it was only 26.45%, comparable to the control sample (CW–control)—19.45%. Interestingly, the number of proteobacteria decreased in the CW–dig. sample (17.47%), but increased in the CW + PLA sample (39.82%) compared to the CW–control sample (32.70%). The analysis of biofilm formation dynamics using the BioFlux microfluidic system shows a significantly faster growth of the biofilm surface area for the CW + PLA sample. This information was complemented by observations of the morphological characteristics of the microorganisms using fluorescence microscopy. The images of the CW + PLA sample showed carrier sections covered with microbial consortia.

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Chitosan/Perlite System as a Microbial Carrier in Anaerobic Digestion of Food Waste: Characteristics and Impact of the Additive Materials

Pilarska,

Marzec-Grządziel,

Makowska

et al. 2024

Preprint

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The article aims to present the results of research on anaerobic digestion (AD) of waste wafers (WF - control) and co-substrate system - waste wafers and cheese (WFC - control), combined with digested sewage sludge. The aim of the study was to evaluate the physicochemical parameters of the chitosan/perlite (Ch/P; 3:1) carrier material and to verify its effect on the directions of change of the bacterial microbiome, removal kinetics of organic matter and AD process efficiency. The experiment was conducted in a laboratory, in a periodical mode of operation of bioreactors, under mesophilic conditions. The results of analyses of morphological-dispersive, spectroscopic, adsorption, thermal and microbiological properties confirmed that the tested carrier material can be an excellent option to implement in biotechnological processes, especially in anaerobic digestion. The microstructural properties of the carrier were influenced by both components: perlite determined the development of the specific surface area, while chitosan shaped the porosity of the system. The thermal properties were determined by the less heat-resistant component, present in a threefold higher weight proportion, i.e. chitosan. The evaluation of quantitative and qualitative changes in the genetic diversity of bacterial communities, carried out using Next Generation Sequencing (NGS), showed that the material has a modifying effect on the bacterial microbiome. Amount of bacteria from phyla Actinobacteria, Bacteroidetes, Campilobacterota, Chloroflexi, Euryarchaeota, Planctomycetes, and Proteobacteria decreased while Firmicutes, Synergistetes, and Thermotogae increased during the course of the experiment. The shapes of the FT-IR spectra indicated a dependence of the degradation rate on both the presence of the carrier and the cosubstrate system. Monitoring of the course of AD was carried out by measuring key parameters for the stability of the process: pH, VFA and VFA/TA ratio (volatile fatty acids/total alkalinity). As a result, an increase in the volume of biogas/methane produced, under the influence of the carrier, was recorded for WF-control by 12.05% and for WFC-control by 19.16%. The volume of methane for the WF-control increased from 351.72 m3 Mg-1 VS to 411.14 m3 Mg-1 VS, while for the cosubstrate sample it increased from 476.84 m3 Mg-1 VS to 518.08 m3 Mg-1 VS, confirming the validity of combining the respective cosubstrate with microbial carrier in anaerobic bioreactor.

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Quantitative and Qualitative Changes in the Genetic Diversity of Bacterial Communities in Anaerobic Bioreactors with the Diatomaceous Earth/Peat Cell Carrier

Cited by 4 publications

References 71 publications

Polylactide (PLA) as a Cell Carrier in Mesophilic Anaerobic Digestion—A New Strategy in the Management of PLA

Polylactide (PLA) as a Cell Carrier in Mesophilic Anaerobic Digestion—A New Strategy in the Management of PLA

Biofilm Formation and Genetic Diversity of Microbial Communities in Anaerobic Batch Reactor with Polylactide (PLA) Addition

Chitosan/Perlite System as a Microbial Carrier in Anaerobic Digestion of Food Waste: Characteristics and Impact of the Additive Materials

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