Quantitative and Qualitative Deficiency of T-Lymphocyte Colony Formation in T-Cell Lymphoma-Leukaemia

Abstract

T-lymphocyte colony formation in a patient with T-cell lymphoma-leukaemia was found to be defective in three ways. Colony numbers in patient cultures were reduced below those of 20 normal donors. Colony size in patient cultures was uniformly small. Colony formation by patient cells was inhibited in co-cultures of patient and normal cells. These observations indicate that the leukaemic T cells in this rare entity have a defective T-cell function.

Depletion of T‐lymphocyte subsets using monoclonal antibody and complement: Effect on T‐colony formation

“…Using the linear relationship between T-colony formation and cell concentration for up to 1 X lo5 cells/plate [16], we extrapolated the results for T-colony formation from OKT4+-depleted and OKT8+-depleted mononuclear cells to estimate T-colony formation from 1 X 105 viable Tlymphocytes for each set of cell preparations (Fig. 2).…”