1997
DOI: 10.1007/s004210050176
|View full text |Cite
|
Sign up to set email alerts
|

Quantitative assessment of degenerative changes in soleus muscle after hindlimb suspension and recovery

Abstract: The aim of this study was to quantify the degenerative and regenerative changes in rat soleus muscle resulting from 3-week hindlimb suspension at 45 degrees tilt (HS group, n = 8) and 4-week normal cage recovery (HS-R group, n = 7). Degenerative changes were quantified by microscope examination of muscle cross sections, and the myosin heavy chain (MHC) composition of soleus muscles was studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis. At the end of 3-week hindlimb suspension, histological … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

2
18
0

Year Published

1997
1997
2020
2020

Publication Types

Select...
7

Relationship

1
6

Authors

Journals

citations
Cited by 17 publications
(20 citation statements)
references
References 26 publications
2
18
0
Order By: Relevance
“…In our study, endomysial accumulation of tenascin-C in unloaded and reloaded soleus muscles was associated with centrally nucleated fibers (see Table 1, Fig. 4, arrowheads), which may reflect internalized myonuclei of regenerating fibers or may indicate infiltrating immune cells (7,50,61). Moreover, tenascin-C accumulation was observed in occasionally infiltrated and split muscle fibers of 5-to 14-day reloaded soleus muscles (Fig.…”
Section: Discussionmentioning
confidence: 53%
See 1 more Smart Citation
“…In our study, endomysial accumulation of tenascin-C in unloaded and reloaded soleus muscles was associated with centrally nucleated fibers (see Table 1, Fig. 4, arrowheads), which may reflect internalized myonuclei of regenerating fibers or may indicate infiltrating immune cells (7,50,61). Moreover, tenascin-C accumulation was observed in occasionally infiltrated and split muscle fibers of 5-to 14-day reloaded soleus muscles (Fig.…”
Section: Discussionmentioning
confidence: 53%
“…Reloading of 10-to 12.5-day hindlimb-suspended rats leads, presumably as a consequence of acute fiber damage, to transient infiltration of damaged soleus muscle fibers with macrophages (and monocytes) within the first 2 days (43,65). With longer duration (Ͼ4 days) of reloading the atrophied rat soleus muscle, an increase in regenerating fibers, arising subsequent to fiber damage (6,7,20), is observed when the number of invaded muscle fibers has returned to the control level (43,61). Morphologically, the acute and chronic muscle fiber damage (and eventual regeneration) is indicated by the presence of fibers with central nuclei (50,61).…”
mentioning
confidence: 99%
“…Myofibers with central nuclei have generally been believed to be regenerating and impairedregeneration fibers [28] with small caliber, but we [62] and recently other authors [4] have questioned this fixed idea. Interestingly, many authors have presented pathological micrographs of diseased muscles and morphometric data, without concentrating on the existence of large centronucleated fibers.…”
Section: Centronucleation Is Not a Marker For Regenerated Fibersmentioning
confidence: 87%
“…Quantitative assessment of HS-induced damage was performed according to the method described by Lexell et al (1992) and as in previous experiments (Bigard et al 1997). Each SOL muscle was divided into ®ve equal parts by transverse sections.…”
Section: Histologymentioning
confidence: 99%
“…However, short-term hindlimb reloading elicited a signi®cant increase in both the number and size of activated macrophages (Krippendorf and Riley 1993), and ®bre disruption (Kasper 1995). After 4 weeks of normal cage recovery from HS, %16% of SOL muscle ®bres showed histological changes normally observed in regenerating ®bres (Bigard et al 1997). It has been suggested previously that a training program is required for recovery both of the SOL muscle mass (Kasper et al 1990) and the oxidative enzyme capacity (Desplanches et al 1987), whereas superimposing exercise training on normal cage recovery increased the incidence of ®bre damage (Kasper et al 1990).…”
Section: Introductionmentioning
confidence: 99%