Quantitative Assessment of Emphysema Severity in Histological Lung Analysis

Marcos, J. Víctor; Muñoz-Barrutia, Arrate; Ortiz‐de‐Solórzano, Carlos; Cristóbal, Gabriel

doi:10.1007/s10439-015-1251-5

Cited by 5 publications

(2 citation statements)

References 30 publications

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“…At least three fields were considered per microscope slide. The Lm was calculated by dividing the total horizontal length of all the lines by the total number of alveolar septa that intercepted the grid lines [60,61]. Masson's trichrome staining was used to measure the peribronchial fibrosis in lung tissue under 10× magnification.…”

Section: Lung Histological Assessmentmentioning

confidence: 99%

Deflamin Attenuated Lung Tissue Damage in an Ozone-Induced COPD Murine Model by Regulating MMP-9 Catalytic Activity

Baltazar-García,

Vargas-Guerrero,

Lima

et al. 2024

IJMS

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Chronic obstructive pulmonary disease (COPD) is comprised of histopathological alterations such as pulmonary emphysema and peribronchial fibrosis. Matrix metalloproteinase 9 (MMP-9) is one of the key enzymes involved in both types of tissue remodeling during the development of lung damage. In recent studies, it was demonstrated that deflamin, a protein component extracted from Lupinus albus, markedly inhibits the catalytic activity of MMP-9 in experimental models of colon adenocarcinoma and ulcerative colitis. Therefore, in the present study, we investigated for the first time the biological effect of deflamin in a murine COPD model induced by chronic exposure to ozone. Ozone exposure was carried out in C57BL/6 mice twice a week for six weeks for 3 h each time, and the treated group was orally administered deflamin (20 mg/kg body weight) after each ozone exposure. The histological results showed that deflamin attenuated pulmonary emphysema and peribronchial fibrosis, as evidenced by H&E and Masson’s trichrome staining. Furthermore, deflamin administration significantly decreased MMP-9 activity, as assessed by fluorogenic substrate assay and gelatin zymography. Interestingly, bioinformatic analysis reveals a plausible interaction between deflamin and MMP-9. Collectively, our findings demonstrate the therapeutic potential of deflamin in a COPD murine model, and suggest that the attenuation of the development of lung tissue damage occurs by deflamin-regulated MMP-9 catalytic activity.

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Section: Lung Histological Assessmentmentioning

confidence: 99%

Deflamin Attenuated Lung Tissue Damage in an Ozone-Induced COPD Murine Model by Regulating MMP-9 Catalytic Activity

Baltazar-García,

Vargas-Guerrero,

Lima

et al. 2024

IJMS

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“…9 Emphysema is the dilation of terminal bronchioles and alveoli due to oxidative damage, tissue remodelling and fibrosis of alveolar walls by environmental irritants and pollutants. [10][11][12][13] An increase in inflammatory cells, erythrocytes within the alveolar capillaries and re-arranged collagen fibres are histopathological features. 11 Idiopathic pulmonary fibrosis is a chronic interstitial pulmonary disease that occurs due to alveolar inflammation that results in epithelial damage, repair, and remodelling of the pulmonary tissue with progression to fibrosis.…”

Section: Introductionmentioning

confidence: 99%

Adverse pulmonary effects after oral exposure to copper, manganese and mercury, alone and in mixtures, in a Spraque-Dawley rat model

Draper

Rooy

et al. 2023

Ultrastructural Pathology

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The rise in respiratory disease has been attributed to an increase in environmental pollution.Heavy metals contribute to environmental contamination via air, water, soil and food. The effects of atmospheric exposure to heavy metals on pulmonary structure and function have been researched, but the effects through drinking water have been neglected. The aim of this study was to investigate the potential in vivo alterations in the pulmonary tissue of male Sprague-Dawley rats after a 28-day oral exposure to copper (Cu), manganese (Mn) and mercury (Hg), alone and in mixtures, at 100 times the World Health Organisation's (WHO) safety limit for each heavy metal in drinking water. Forty-eight male Sprague-Dawley rats were randomly divided into eight groups (n=6): control, Cu, Mn, Hg, Cu + Mn, Cu + Hg, Mn + Hg and Cu, Mn + Hg. The morphology of lung tissue and the bronchioles were evaluated using light-and transmission electron microscopy. For all exposed groups, morphological changes included thickened inter-and intra-alveolar spaces, stratified epithelium, disrupted smooth muscle and early fibrosis and desquamation of the epithelia of the bronchioles to varying degrees. In all exposed groups, ultrastructurally, an increase in disarranged collagen and elastin fibres, nuclear membrane detachment, chromatin condensation, indistinct nucleoli and an increase in collagen fibre disarrangement was observed. This study has identified that oral exposure to Cu, Mn and Hg and as part of mixtures caused pathogenesis due to inflammation, cellular damage and fibrosis with Mn + Hg being the most potent heavy metal group.

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Practical Methods for Assessing Emphysema Severity Based on Estimation of Linear Mean Intercept (Lm) in the Context of Animal Models of Alpha-1 Antitrypsin Deficiency

Davis

Thane

Hoffman

2017

Methods in Molecular Biology

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Alpha-1 antitrypsin deficiency is typified by panacinar emphysema in humans. Whilst animal models of (α1A-TD) that more accurately reflect the histology and molecular pathology of α1A-TD are in development, it is timely to discuss methods to assess emphysema severity. Several methods exist to quantify emphysema from histologic sections, including linear mean intercept (Lm), equivalent diameters (D) or their statistical derivatives (D2), and more recently probability models of D2 ("severity index"). Given proper attention to lung inflation, reference volume, and random sampling, Lm determined by intersect point counting provides a robust analytical tool to quantify emphysema severity. Details of lung preparation, processing for random sampling, and batch processing of prescreened images are provided herein.

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Quantitative Assessment of Emphysema Severity in Histological Lung Analysis

Cited by 5 publications

References 30 publications

Deflamin Attenuated Lung Tissue Damage in an Ozone-Induced COPD Murine Model by Regulating MMP-9 Catalytic Activity

Deflamin Attenuated Lung Tissue Damage in an Ozone-Induced COPD Murine Model by Regulating MMP-9 Catalytic Activity

Adverse pulmonary effects after oral exposure to copper, manganese and mercury, alone and in mixtures, in a Spraque-Dawley rat model

Practical Methods for Assessing Emphysema Severity Based on Estimation of Linear Mean Intercept (Lm) in the Context of Animal Models of Alpha-1 Antitrypsin Deficiency

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