Quantitative assessment of infarct size in vivo by myocardial contrast echocardiography in a murine acute myocardial infarction model

Park, Seung Woo; Lee, Sung Yun; Park, Sun Jin; Lee, Sang‐Chol; Gwon, Hyeon‐Cheol; Kim, Duk Kyung

doi:10.1016/j.ijcard.2003.10.034

Cited by 18 publications

(14 citation statements)

References 26 publications

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“…In many published studies exploring these potential therapies, success has commonly been evaluated by determination of infarct size, as well as by functional and other histological parameters. In experiments involving an acute MI setting, infarct size is typically based on histological measurement of the area of the infarcted region in tissue sections of the left ventricle (LV) (13,16,20,26). In contrast, histological measurement of the arc length of the infarct scar has been commonly used in a chronic MI setting (8,14,18,19).…”

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confidence: 99%

Myocardial infarct size measurement in the mouse chronic infarction model: comparison of area- and length-based approaches

Takagawa¹,

Zhang²,

Wong³

et al. 2007

Journal of Applied Physiology

278

244

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Myocardial infarct size measurement in the mouse chronic infarction model: comparison of area-and length-based approaches. J Appl Physiol 102: 2104 -2111, 2007. First published March 8, 2007; doi:10.1152/japplphysiol.00033.2007.-Efficacy of potential treatments for myocardial infarction (MI) is commonly assessed by histological measurement of infarct size in rodent models. In experiments involving an acute MI setting, measurement of the infarcted area in tissue sections of the left ventricle is a standard approach to determine infarct size. This approach has also been used in the chronic infarct setting to measure infarct area several weeks post-MI. We tested the hypothesis that, because wall thinning is known to occur in the chronic setting, the area measurement approach would be less appropriate. We compared infarct measurements in tissue sections based on 1) infarct area, 2) epicardial and endocardial infarct arc lengths, and 3) midline infarct arc length. Infarct sizes from all three measurement approaches correlated significantly with left ventricular ejection fraction and wall motion abnormality. However, the infarct size values derived from the area measurement approach were significantly smaller than those from the other two measurement approaches, and the range of values obtained was compressed 0.4-fold. The midline method allowed detection of the expected size differences between infarcts of variable severity resulting from proximal vs. distal ligation of the coronary artery. Segmental infarct size was correlated with segmental wall motion abnormality. We conclude that both area-and length-based measurements can be used to determine relative infarct size over a wide range of severity, although the area-based measurements are substantially more compressed due to wall thinning, and that the estimation of infarct midlines is a simple, reliable approach to infarct size assessment. cardiac remodeling; area-based measurement; length-based measurement; histology RODENT MODELS OF MYOCARDIAL infarction (MI) have been frequently used to elucidate the pathophysiological and molecular mechanisms of cardiac remodeling after the onset of MI (5,7,18,19,23,24). In recent years, the delivery of potentially therapeutic genes and the implantation of stem cells have attracted much interest and have been evaluated with the use of rat or mouse MI models (2,4,9,12,14,21,22). In many published studies exploring these potential therapies, success has commonly been evaluated by determination of infarct size, as well as by functional and other histological parameters. In experiments involving an acute MI setting, infarct size is typically based on histological measurement of the area of the infarcted region in tissue sections of the left ventricle (LV) (13,16,20,26). In contrast, histological measurement of the arc length of the infarct scar has been commonly used in a chronic MI setting (8,14,18,19). Although it is well known that there is progressive thinning of the infarcted wall with reduction in the volume of the infarcted...

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mentioning

confidence: 99%

Myocardial infarct size measurement in the mouse chronic infarction model: comparison of area- and length-based approaches

Takagawa¹,

Zhang²,

Wong³

et al. 2007

Journal of Applied Physiology

278

244

View full text Add to dashboard Cite

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“…Among the various techniques that are applied to assess the impaired cardiac function after experimental MI, transthoracic echocardiography is a reliable and noninvasive method for serially monitoring the changes of LV geometry and function [20,21]. Furthermore, MCE, a technique that has been proven as a rational tool for assessing myocardial perfusion and quantifying the infarct area in MI rats [22][23][24], is also utilized. And such a combination created an efficient, accurate and rapid method of in vivo cardiovascular measurement in small animals.…”

Section: Discussionmentioning

confidence: 99%

A new hydrodynamic approach by infusion of drag-reducing polymers to improve left ventricular function in rats with myocardial infarction

Chen

Zha

Xiu

et al. 2011

International Journal of Cardiology

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“…(1) measuring the area of the infarcted region [23][24][25] or (2) measuring the arc length of the infarcted region [7,26]. The area-based method is often used with early stage infarcts.…”

Section: Quantification Of Infarct Size and Thresholdingmentioning

confidence: 99%

Characterisation of the mechanical properties of infarcted myocardium in the rat under biaxial tension and uniaxial compression

Sirry

Butler

Patnaik

et al. 2016

Journal of the Mechanical Behavior of Biomedical Materials

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Characterisation of the mechanical properties of infarcted myocardium in the rat under biaxial tension and uniaxial compression, Journal of the Mechanical Behavior of Biomedical Materials, http://dx.doi.org/10.1016Materials, http://dx.doi.org/10. /j.jmbbm.2016 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractUnderstanding the passive mechanical properties of infarcted tissue at different healing stages is essential to explore the emerging biomaterial injection-based therapy for myocardial infarction (MI). Although rats have been widely used as animal models in such investigations, the data in literature that quantify the passive mechanical properties of rat heart infarcts is very limited. MI was induced in rats and hearts were harvested immediately (0 day), 7, 14 and 28 days after infarction onset. Left ventricle anterioapical samples were cut and underwent equibiaxial and non equibiaxial tension followed by uniaxial compression mechanical tests. Histological analysis was conducted to confirm MI and to quantify the size of the induced infarcts. Infarcts maintained anisotropy and the nonlinear biaxial and compressive mechanical behaviour throughout the healing phases with the circumferential direction being stiffer than the longitudinal direction.Mechanical coupling was observed between the two axes in all infarct groups. The 0, 7, 14 and 28 days infarcts showed 438, 693, 1048 and 1218 kPa circumferential tensile moduli. The 28 day infarct group showed a significantly higher compressive modulus compared to the other infarct groups (p= 0.0060, 0.0293, and 0.0268 for 0, 7 and 14 days groups). Collagen fibres were found to align in a preferred direction for all infarct groups supporting the observed mechanical anisotropy. The presented data are useful for developing material models for healing infarcts and for setting a baseline for future assessment of emerging mechanical-based MI therapies.

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Quantitative assessment of infarct size in vivo by myocardial contrast echocardiography in a murine acute myocardial infarction model

Cited by 18 publications

References 26 publications

Myocardial infarct size measurement in the mouse chronic infarction model: comparison of area- and length-based approaches

Myocardial infarct size measurement in the mouse chronic infarction model: comparison of area- and length-based approaches

A new hydrodynamic approach by infusion of drag-reducing polymers to improve left ventricular function in rats with myocardial infarction

Characterisation of the mechanical properties of infarcted myocardium in the rat under biaxial tension and uniaxial compression

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