Quantitative assessment of phagocytic activity of hemocytes in the prawn,Penaeus merguiensis, by flow cytometric analysis

White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis

“…Microscopic examination of hemolymph of penaeid shrimps has shown that the GCs and HCs are represented equally, whereas SGCs make up about 10% of the total hemocyte population (Lee et al 2001). In the present study, flow cytometry analysis showed a ratio of hemocytes similar to that reported by Lee et al on Day 1 of the experiment.…”

“…The HCs were unstable hemocytes, nonphagocytic, and were primarily involved in coagulation of hemolymph; both GCs and SGCs are actively phagocytic (Omori et al 1989, Gargioni & Barracco 1998, Hose et al 1990). In contrast, Lee et al (2001) demonstrated that both HCs and SGCs are potentially phagocytic. Although the role of HCs in the shrimp's defence mechanim is not clear, GCs and SGCs inevitably played a major role.…”

“…The flow cytometry approach employed in this study offers several advantages compared to microscopy, as it is possible to analyze individual cell types, and to sort and harvest a particular kind of cell within a short period of time. Recently, this approach has been used in several studies of shrimp hemocytes (Sequeira et al 1996, Lee et al 2001. Using a cell sorter, we have sorted and harvested each type of the 3 hemocytes for further study.…”

“…3a. These cells were defined as granular cells (GCs), semigranular cells (SGCs) and hyaline cells (HCs) (Lee et al 2001). Flow cytometric studies revealed that the density of different types of hemocytes altered during the course of viral infection.…”

Chemopreventive Effect of Lactobacttlus rhamnosus on Growth of a Subcutaneously Implanted Bladder Cancer Cell Line in the Mouse

“…The assay was carried out using Escherichia coli strain (E. coli) K12, as described by Lee et al, 13) except that incubation was carried out at 37°C, and quenching of the red fluorescence of propidium iodide-stained membrane-bound and non-engulfed E. coli was performed using 100 µl of trypan blue (obtained from PhagoTest, OPREGEN, Gesellschaft für Biotechnologische Forschung, Entwicklung und Produktion m.b.H., Heidelberg, Germany). Evaluation of spleen cell suspension by flow cytometry Mice were sacrificed 24 h after the last treatment.…”