Quantitative Assessment of Protein Adsorption by Combination of the Enzyme-Linked Immunosorbent Assay with Radioisotope-Based Studies

In vitro haematic proteins adsorption and cytocompatibility study on acrylic copolymer to realise coatings for drug-eluting stents

“…Adsorption kinetics of albumin and fibrinogen were in line with other papers in literature. In particular, for albumin a behaviour similar to the one reported in this work was well explained with models proposing a monolayer of irreversible adsorbed proteins onto surfaces [22] and the following increase in the amount of protein adsorption after the formation of a complete monolayer could be obtained by a rearrangement of the protein molecules on the surface [23,24]. High asymmetric proteins can significantly change the contact area by changing the molecule orientation and it certainly enhances the possibility to increase the amount of protein adsorbed on the surface after the formation of the first monolayer.…”

Quantitative analysis of binary adsorbed protein films by time of flight secondary ion mass spectrometry

“…1,2 Typically, analysis of multicomponent adsorbed protein films involves a labeling step for each of the proteins detected, such as radiolabel-ing 3,4 or fluorescence labeling combined with total internal reflection fluorescence 5,6 or fluorescence microscopy. 7 Detection methods utilizing a reaction step such as ELISA, 8 which can be coupled with surface plasmon resonance 9 or ellipsometry, 10,11 are also common. Elution of the adsorbed proteins and subsequent electrophoretic and immunoblotting analysis has also been used to analyze complex adsorbed protein films.…”

“…12 ELISA experiments pose difficulties for quantification of the amounts of adsorbed protein because the results are related to a reference material. 8 Recent developments in surface matrix-assisted laser desorption ionization (MALDI) mass spectrometry has enhanced the ability to detect adsorbed proteins in complex mixtures, 13 but this technique requires the application of a photoactive matrix and is not able to detect very large proteins. 14 Static time of flight secondary ion mass spectrometry (ToF-SIMS) is a highly specific and surfacesensitive technique ideally suited for the analysis of adsorbed protein films.…”

Protein–material interactions: From micro-to-nano scale

“…There are various, more or less elaborate techniques to do this, like ellipsometry or quartz crystal microbalance -to which we will refer explicitly latter -but the simplest way is by labeling the protein with radioisotopes [33,34] or fluorescent probes [35]. Both methods are based on the quantitation of a signal, either radioactivity or fluorescence, emitted by labeled proteins adsorbed on the sample.…”