Purpose Although kinematic changes in the sagittal plane of the osteoarthritic knee (OA) have been elucidated, very few studies have analysed changes in the frontal and horizontal planes. Therefore, the aim of this study was to investigate in vivo 3D knee kinematics during walking in patients wth knee OA. Methods Thirty patients with medial knee OA and a control group of similarly aged individuals were prospectively collected for this study. All participants were assessed with KneeKG TM system while walking on a treadmill at a selfselected speed. In each trial, we calculated the angular displacment of flexion/extension, abduction/adduction and external/internal tibial rotation. Statistical analysis was performed to determine differences between the knee OA group and the control group.