Quantitative Assessment of Transition Proteins 1, 2 Spermatid-Specific Linker Histone H1-Like Protein Transcripts in Spermatozoa from Normozoospermic and Asthenozoospermic Men

Jȩdrzejczak, Piotr; Kempisty, Bartosz; Bryja, Artur; Mostowska, M.; Depa-Martynów, Magdalena; Pawełczyk, Leszek; Jagodzińśki, Paweł P.

doi:10.1080/01485010701426430

Cited by 36 publications

(21 citation statements)

References 33 publications

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“…mRNA of the TSSK6 gene deleted in azoospermia like (DAZL) and Pumilio-2 (PUM2), which are essential for the germ cell development (Fox et al, 2005). Expression of TNP1 and 2 were significantly lower in spermatozoa from asthenozoospermic men compared to normal (Jedrzejczak et al, 2007).…”

Section: Role Of Sperm Rnamentioning

confidence: 99%

Sperm RNA: a New Class of Fertility Biomarkers for Birds

Shafeeque

2014

Adv. Anim. Vet. Sci.

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Leeuwenhoek discovered and named spermatozoa but could not convince the scientific world the potentiality of the sperm. Similarly, transcriptionally quiescent genetic material was marked by a state of tranquil among researchers until the breakthrough of RNA transcript residing inside the sperm. Even though, queries and enigmas highlight throughout sperm research, most reports favor us in thinking about role of these transcripts in fertilization and early embryonic development. Many sperm transcripts have been confirmed as bio-fertility marker in human and other mammalian specie. With this review, we are attempting to summarize spermatozoal work conducted so far in other vertebrate classes and thus representing a potential to serve as bio-fertility marker through such a non-invasive approach in birds. All copyrights reserved to Nexus® academic publishers

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Section: Role Of Sperm Rnamentioning

confidence: 99%

Sperm RNA: a New Class of Fertility Biomarkers for Birds

Shafeeque

2014

Adv. Anim. Vet. Sci.

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“…HILS1 protein localisation within elongating spermatids overlaps only minimally with H1T, but substantially with the TPs and PRMs (Yan et al 2003, Iguchi et al 2004. At least one study indicates that decreased HILS1 expression in sperm is associated with asthenozoospermia (Jedrzejczak et al 2007). …”

Section: Epigenetic Regulation In Male Germ Cellsmentioning

confidence: 99%

Epigenetic regulation in male germ cells

Zamudio¹,

Chong²,

O'Bryan³

2008

Reproduction

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In recent years, it has become increasingly clear that epigenetic regulation of gene expression is critical during spermatogenesis. In this review, the epigenetic regulation and the consequences of its aberrant regulation during mitosis, meiosis and spermiogenesis are described. The current knowledge on epigenetic modifications that occur during male meiosis is discussed, with special attention on events that define meiotic sex chromosome inactivation. Finally, the recent studies focused on transgenerational and paternal effects in mice and humans are discussed. In many cases, these epigenetic effects resulted in impaired fertility and potentially long-ranging affects underlining the importance of research in this area.

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“…Sperm mRNAs encoding for protamines have been largely studied and could be considered as candidate markers for the discrimination between fertile and infertile men [38] or between normozoospermic and asthenozoospermic men [21]. The quantitative assessment of Hils1, transition proteins 1 and 2 mRNAs by real-time quantitative PCR has shown a reduction of these transcripts in asthenozoospermic compared to normozoospermic men [19]. Only protamines (but not HILS1) and fertilin beta mRNA levels are well correlated to the embryo quality [7].…”

Section: Human Sperm Transcripts As Candidate Markers Of Infertilitymentioning

confidence: 99%

“…A wide range of transcripts synthetized either throughout the early spermatogenesis or during spermiogenesis has been found to be stored in translationally repressed ribonucleoproteins in round spermatids before their delayed activation for translation during spermiogenesis. During the last two decades numerous works have revealed an extended pattern of mRNA molecules using RT-PCR and/or in situ hybridization techniques [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30], but also the presence of small RNAs (microRNAs, siRNAs) in the human spermatozoa [for review 5].…”

Section: Human Sperm Transcripts As Candidate Markers Of Infertilitymentioning

confidence: 99%

New insights about the evaluation of human sperm quality: the aromatase example.

Galeraud-Denis

Travert²,

Vienne³

et al. 2010

Folia Histochem Cytobiol.

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Male contribution to the couple's infertility is at first evaluated by the routine examination of semen parameters upon optical microscopy providing valuable information for a rational initial diagnosis and for a clinical management of infertility. But the different forms of infertility defined according to the WHO criteria especially teratozoospermia are not always related to the chromatin structure or to the fertilization capacity. New investigations at the molecular level (transcript and protein) could be developed in order to understand the nature of sperm malformation responsible of human infertility and thus to evaluate the sperm quality. The profile analysis of spermatozoal transcripts could be considered as a fingerprint of the past spermatogenic events. The selection of representative transcripts of normal spermatozoa remains complex because a differential expression (increased, decreased or not modified levels) of specific transcripts has been revealed between immotile and motile sperm fractions issued from normozoospermic donors. Microarrays tests or real-time quantitative PCR could be helpful for the identification of factors involved in the male infertility. Differences in the expression of specific transcripts have been reported between normal and abnormal semen samples. With the aromatase example, we have noted a negative strong correlation between the amount of transcript and the percentage of abnormal forms especially in presence of head defects. Immunocytochemical procedures using fluorescent probes associated with either confocal microscopy or flow cytometry can be also helpful to proceed with further investigations about the localization of proteins in the compartmentalized spermatozoa or the acrosome reaction. The dual location of aromatase both in the equatorial segment, the mid-piece and the tail could explain the double role of this enzyme in acrosome reaction and motility.

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Quantitative Assessment of Transition Proteins 1, 2 Spermatid-Specific Linker Histone H1-Like Protein Transcripts in Spermatozoa from Normozoospermic and Asthenozoospermic Men

Cited by 36 publications

References 33 publications

Sperm RNA: a New Class of Fertility Biomarkers for Birds

Sperm RNA: a New Class of Fertility Biomarkers for Birds

Epigenetic regulation in male germ cells

New insights about the evaluation of human sperm quality: the aromatase example.

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