Quantitative Assessment of Ultrastructure and Light Scatter in Mouse Corneal Debridement Wounds

Boote, Craig; Du, Yiqin; Morgan, Siân R.; Harris, Jonathan L.; Kamma-Lorger, Christina S.; Hayes, Sally; Lathrop, Kira L.; Roh, Danny S.; Burrow, Michael; Terrill, Nicholas J.; Funderburgh, James L.; Meek, Keith Michael Andrew

doi:10.1167/iovs.11-9305

Cited by 56 publications

(64 citation statements)

References 42 publications

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“…30 At baseline, the adult BALB/c cornea is approximately 10% thicker than the C57BL/6 cornea. 31 Our data show strain-dependent differences in baseline corneal thickness that support this study.…”

Section: Discussionmentioning

confidence: 97%

Monitoring of Strain-Dependent Responsiveness to TLR Activation in the Mouse Anterior Segment Using SD-OCT

Downie¹,

Stainer²,

Chinnery³

2014

Investigative Ophthalmology & Visual Science

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Section: Discussionmentioning

confidence: 97%

Monitoring of Strain-Dependent Responsiveness to TLR Activation in the Mouse Anterior Segment Using SD-OCT

Downie¹,

Stainer²,

Chinnery³

2014

Investigative Ophthalmology & Visual Science

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“…Wounds were generated by debridement of epithelium and anterior stromal tissue with a rotating burr, resulting in deposition of opaque scar tissue, accompanied by loss of stromal lamellar organization, large and disorganized collagen fibrils, and deposition of collagen type III, hyaluronan, and fibronectin. 53 When CSSCs were layered on the surface of the cornea in fibrin gel at the time of wounding, the debrided stromal tissue was replaced with ECM containing normal collagen organization and fibril diameter. Fibrotic ECM components were absent and corneal transparency was normal.…”

Section: Stem Cells In the Stromamentioning

confidence: 99%

Stem Cells in the Limbal Stroma

Funderburgh

2016

The Ocular Surface

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110

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The corneal stroma contains a population of mesenchymal cells subjacent to the limbal basement membrane with characteristics of adult stem cells. These ‘niche cells’ support limbal epithelial stem cell viability. In culture by themselves, the niche cells display a phenotype typical of mesenchymal stem cells. These stromal stem cells exhibit a potential to differentiate to multiple cell types, including keratocytes, thus providing an abundant source of these rare cells for experimental and bioengineering applications. Stromal stem cells have also shown the ability to remodel pathological stromal tissue, suppressing inflammation and restoring transparency. Because stromal stem cells can be obtained by biopsy, they offer a potential for autologous stem cell treatment for stromal opacities. This review provides an overview of the status of work on this interesting cell population.

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“…An example is shown in Figure 4, where stromal haze is assessed in the mouse model following mechanical debridement ( MD ) injury with removal of basement membrane, as well as transcorneal freeze injury ( FI ). 49–52 MD in the mouse induces fibrosis both within and on top of the injured stroma, similar to PRK (Figure 4B). 52 FI in the mouse results in cell loss throughout the full thickness of the cornea, and also induces stromal keratocyte activation (Figure 4C).…”

Section: Full-thickness 3-d Corneal Imaging and Analysismentioning

confidence: 66%

“…49–52 MD in the mouse induces fibrosis both within and on top of the injured stroma, similar to PRK (Figure 4B). 52 FI in the mouse results in cell loss throughout the full thickness of the cornea, and also induces stromal keratocyte activation (Figure 4C). 49,50 In both injury models, an increase in cell and ECM backscattering in the cornea is identified (shaded areas under curves, “area” on top right of each image) as compared to a preoperative scan (Figure 4A) taken with the same “gain” setting (same position of horizontal slider under “image quality” readout on HRT-RCM software screen).…”

Section: Full-thickness 3-d Corneal Imaging and Analysismentioning

confidence: 66%

“…The epithelium and basement membrane were then removed using an Algerbrush as previously described. 52 A relative estimate of stromal cell and ECM backscattering was obtained by measuring the area under the CMTF curve as previously described (shaded areas under curves, “area” on top right of each image). Note the increase in backscattering following injury.…”

Section: Figurementioning

confidence: 99%

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In Vivo Confocal Microscopy of the Cornea: New Developments in Image Acquisition, Reconstruction, and Analysis Using the HRT-Rostock Corneal Module

Petroll

Robertson

2015

The Ocular Surface

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The optical sectioning ability of confocal microscopy allows high magnification images to be obtained from different depths within a thick tissue specimen, and is thus ideally suited to the study of intact tissue in living subjects. In vivo confocal microscopy has been used in a variety of corneal research and clinical applications since its development over 25 years ago. In this article we review the latest developments in quantitative corneal imaging with the Heidelberg Retinal Tomograph with Rostock Corneal Module (HRT-RCM). We provide an overview of the unique strengths and weaknesses of the HRT-RCM. We discuss techniques for performing 3-D imaging with the HRT-RCM, including hardware and software modifications that allow full thickness confocal microscopy through focusing (CMTF) of the cornea, which can provide quantitative measurements of corneal sublayer thicknesses, stromal cell and extracellular matrix backscatter, and depth dependent changes in corneal keratocyte density. We also review current approaches for quantitative imaging of the subbasal nerve plexus, which require a combination of advanced image acquisition and analysis procedures, including wide field mapping and 3-D reconstruction of nerve structures. The development of new hardware, software, and acquisition techniques continues to expand the number of applications of the HRT-RCM for quantitative in vivo corneal imaging at the cellular level. Knowledge of these rapidly evolving strategies should benefit corneal clinicians and basic scientists alike.

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Quantitative Assessment of Ultrastructure and Light Scatter in Mouse Corneal Debridement Wounds

Cited by 56 publications

References 42 publications

Monitoring of Strain-Dependent Responsiveness to TLR Activation in the Mouse Anterior Segment Using SD-OCT

Monitoring of Strain-Dependent Responsiveness to TLR Activation in the Mouse Anterior Segment Using SD-OCT

Stem Cells in the Limbal Stroma

In Vivo Confocal Microscopy of the Cornea: New Developments in Image Acquisition, Reconstruction, and Analysis Using the HRT-Rostock Corneal Module

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