Quantitative Bead-Based Flow Cytometry for Assaying Rab7 GTPase Interaction with the Rab-Interacting Lysosomal Protein (RILP) Effector Protein

Agola, Jacob O.; Sivalingam, Daniel; Cimino, Daniel F.; Simons, Peter C.; Buranda, Tione; Sklar, Larry A.; Wandinger‐Ness, Angela

doi:10.1007/978-1-4939-2569-8_28

Cited by 8 publications

(5 citation statements)

References 57 publications

(68 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rab7 facilitates vesicular transport and delivery from early to late endosomes and late endosomes to lysosomes. The role of Rab7 in vesicular transport is dependent on its interactions with effector proteins, among them RILP, which aids in the recruitment of active Rab7 (GTP-bound) onto dynein-dynactin motor complexes to facilitate late endosomal transport on the cytoskeleton (Agola et al, 2015).…”

Section: Lysosomal Proteinsmentioning

confidence: 99%

The CLN3 gene and protein: What we know

Mirza¹,

Vainshtein

DiRonza

et al. 2019

Molec Gen & Gen Med

View full text Add to dashboard Cite

Background One of the most important steps taken by Beyond Batten Disease Foundation in our quest to cure juvenile Batten (CLN3) disease is to understand the State of the Science. We believe that a strong understanding of where we are in our experimental understanding of the CLN3 gene, its regulation, gene product, protein structure, tissue distribution, biomarker use, and pathological responses to its deficiency, lays the groundwork for determining therapeutic action plans. Objectives To present an unbiased comprehensive reference tool of the experimental understanding of the CLN3 gene and gene product of the same name. Methods BBDF compiled all of the available CLN3 gene and protein data from biological databases, repositories of federally and privately funded projects, patent and trademark offices, science and technology journals, industrial drug and pipeline reports as well as clinical trial reports and with painstaking precision, validated the information together with experts in Batten disease, lysosomal storage disease, lysosome/endosome biology. Results The finished product is an indexed review of the CLN3 gene and protein which is not limited in page size or number of references, references all available primary experiments, and does not draw conclusions for the reader. Conclusions Revisiting the experimental history of a target gene and its product ensures that inaccuracies and contradictions come to light, long‐held beliefs and assumptions continue to be challenged, and information that was previously deemed inconsequential gets a second look. Compiling the information into one manuscript with all appropriate primary references provides quick clues to which studies have been completed under which conditions and what information has been reported. This compendium does not seek to replace original articles or subtopic reviews but provides an historical roadmap to completed works.

show abstract

Section: Lysosomal Proteinsmentioning

confidence: 99%

The CLN3 gene and protein: What we know

Mirza¹,

Vainshtein

DiRonza

et al. 2019

Molec Gen & Gen Med

View full text Add to dashboard Cite

show abstract

“…Iem‐spFRET combined with E‐FRET not only shown reagents‐induced interaction between A 1 R and A 2A R changes of whole cell but also visualized partial differences within same cell. Research shows that interactions between proteins often mediate biological mechanisms [57] (e.g., the transport of receptors [58, 59], regulating the metabolites [60], lipid diffusion [61], pro‐inflammatory responses [62]), and may become a potential therapeutic target [63–65]. Therefore, our results on the spatio‐temporal study of A 1 R and A 2A R interaction may contribute to reveal many clues on the function and regulation of A 1 R‐A 2A R heterodimer.…”

Section: Resultsmentioning

confidence: 90%

High spatio‐temporal resolution measurement of A₁R and A_2AR interactions combined with Iem‐spFRET and E‐FRET methods

Lin

Wang

et al. 2021

Journal of Biophotonics

View full text Add to dashboard Cite

A1R‐A2AR heterodimers regulate striatal glutamatergic neurotransmission. However, few researches about kinetics have been reported. Here, we combined Iem‐spFRET and E‐FRET to investigate the kinetics of A1R and A2AR interaction. Iem‐spFRET obtains the energy transfer efficiency of the whole cell. E‐FRET gets energy transfer efficiency with high spatial resolution, whereas, it was prone to biases because background was easily selected due to manual operation. To study the interaction with high spatio‐temporal resolution, Iem‐spFRET was used to correct the deviation of E‐FRET. In this paper, A1R and A2AR interaction was monitored, and the changes of FRET efficiency of the whole or/and partial cell membrane were described. The results showed that activation of A1R or A2AR leads to rapid aggregation, inhibition of A1R or A2AR leads to slow segregation, and the interaction is reversible. These results demonstrated that combination of Iem‐spFRET and E‐FRET could measure A1R and A2AR interaction with high spatio‐temporal resolution.

show abstract

“…Rabs have also been implicated in cancer progression, with the consequence that targeted Rab inhibitors are under development (Salehi et al, 2009; Hong et al, 2010; Agola et al, 2015; Raza et al, 2018). The ubiquitous activity of Rabs in all aspect of intracellular membrane dynamics requires that any such pharmaceutical intervention be tightly directed to the pathologic process.…”

Section: Rabs As Targets For Drug Therapy Of Herpes-associated Cognitmentioning

confidence: 99%

Herpes Simplex Virus, Alzheimer’s Disease and a Possible Role for Rab GTPases

Bearer

2019

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Herpes simplex virus (HSV) is a common pathogen, infecting 85% of adults in the United States. After reaching the nucleus of the long-lived neuron, HSV may enter latency to persist throughout the life span. Re-activation of latent herpesviruses is associated with progressive cognitive impairment and Alzheimer’s disease (AD). As an enveloped DNA virus, HSV exploits cellular membrane systems for its life cycle, and thereby comes in contact with the Rab family of GTPases, master regulators of intracellular membrane dynamics. Knock-down and overexpression of specific Rabs reduce HSV production. Disheveled membrane compartments could lead to AD because membrane sorting and trafficking are crucial for synaptic vesicle formation, neuronal survival signaling and Abeta production. Amyloid precursor protein (APP), a transmembrane glycoprotein, is the parent of Abeta, the major component of senile plaques in AD. Up-regulation of APP expression due to HSV is significant since excess APP interferes with Rab5 endocytic trafficking in neurons. Here, we show that purified PC12-cell endosomes transport both anterograde and retrograde when injected into the squid giant axon at rates similar to isolated HSV. Intracellular HSV co-fractionates with these endosomes, contains APP, Rab5 and TrkA, and displays a second membrane. HSV infected PC12 cells up-regulate APP expression. Whether interference with Rabs has a specific effect on HSV or indirectly affects membrane compartment dynamics co-opted by virus needs further study. Ultimately Rabs, their effectors or their membrane-binding partners may serve as handles to reduce the impact of viral re-activation on cognitive function, or even as more general-purpose anti-microbial therapies.

show abstract

Quantitative Bead-Based Flow Cytometry for Assaying Rab7 GTPase Interaction with the Rab-Interacting Lysosomal Protein (RILP) Effector Protein

Cited by 8 publications

References 57 publications

The CLN3 gene and protein: What we know

The CLN3 gene and protein: What we know

High spatio‐temporal resolution measurement of A₁R and A_2AR interactions combined with Iem‐spFRET and E‐FRET methods

Herpes Simplex Virus, Alzheimer’s Disease and a Possible Role for Rab GTPases

Contact Info

Product

Resources

About

Quantitative Bead-Based Flow Cytometry for Assaying Rab7 GTPase Interaction with the Rab-Interacting Lysosomal Protein (RILP) Effector Protein

Cited by 8 publications

References 57 publications

The CLN3 gene and protein: What we know

The CLN3 gene and protein: What we know

High spatio‐temporal resolution measurement of A1R and A2AR interactions combined with Iem‐spFRET and E‐FRET methods

Herpes Simplex Virus, Alzheimer’s Disease and a Possible Role for Rab GTPases

Contact Info

Product

Resources

About

High spatio‐temporal resolution measurement of A₁R and A_2AR interactions combined with Iem‐spFRET and E‐FRET methods