Quantitative Cerebral Blood Flow Measurements Using MRI

Muir, Eric R.; Watts, Lora Talley; Tiwari, Yash; Bresnen, Andrew; Shen, Qiang; Duong, Tarn

doi:10.1007/978-1-4939-0320-7_17

Cited by 22 publications

(16 citation statements)

References 18 publications

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“…DSC-MRI typically makes use of rapidly acquired MR images after an intravenous bolus injection of a paramagnetic contrast agent 66 Besides the earlier mentioned T1-shortening effect using DCE techniques, paramagnetic contrast agents such as Gd-DTPA also induce T2*-shortening via magnetic susceptibility effects. The temporary T2*-shortening, caused by passage of MR contrast agent through the microvascular bed, can be measured with a FLASH gradient-echo T2*-weighted MRI sequence.…”

Section: Methodsmentioning

confidence: 99%

RETRACTED ARTICLE: Pericyte degeneration causes white matter dysfunction in the mouse central nervous system

Montagne

Nikolakopoulou

Zhao

et al. 2018

Nat Med

328

348

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Section: Methodsmentioning

confidence: 99%

RETRACTED ARTICLE: Pericyte degeneration causes white matter dysfunction in the mouse central nervous system

Montagne

Nikolakopoulou

Zhao

et al. 2018

Nat Med

328

348

View full text Add to dashboard Cite

“…Isolation of bovine brain capillaries: capillaries from calves (<12 months of age) were isolated as described previously [ 16 ]. Briefly, the meninges were removed, and the grey matter scraped off the cortices using a razor blade and homogenized in ice-cold Dulbecco’s Modified Eagle Medium (DMEM) using a 40-mL Dounce tissue grinder set (Merck, St. Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

Conjugation of Therapeutic PSD-95 Inhibitors to the Cell-Penetrating Peptide Tat Affects Blood–Brain Barrier Adherence, Uptake, and Permeation

et al. 2020

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Novel stroke therapies are needed. Inhibition of the interaction between the postsynaptic density-95 (PSD-95)/disc large/ZO-1 (PDZ) domains of PSD-95 and the N-methyl-D-aspartate (NMDA) receptor has been suggested as a strategy for relieving neuronal damage. The peptides NR2B9c and N-dimer have been designed to hinder this interaction; they are conjugated to the cell-penetrating peptide Tat to facilitate blood–brain barrier (BBB) permeation and neuronal uptake. Tat-N-dimer exhibits 1000-fold better target affinity than Tat-NR2B9c, but the same magnitude of improvement is not observed in terms of therapeutic effect. Differences in BBB permeation by Tat-NR2B9c and Tat-N-dimer may explain this difference, but studies providing a direct comparison of Tat-NR2B9c and Tat-N-dimer are lacking. The aim of the present study was therefore to compare the BBB uptake and permeation of Tat-NR2B9c and Tat-N-dimer. The peptides were conjugated to the fluorophore TAMRA and their chemical stability assessed. Endothelial membrane association and cell uptake, and transendothelial permeation were estimated using co-cultures of primary bovine brain capillary endothelial cells and rat astrocytes. In vivo BBB permeation was demonstrated in mice using two-photon microscopy imaging. Tissue distribution was evaluated in mice demonstrating brain accumulation of TAMRA-Tat (0.4% ID/g), TAMRA-Tat-NR2B9c (0.3% ID/g), and TAMRA-Tat-N-dimer (0.25% ID/g). In conclusion, we demonstrate that attachment of NR2B9c or N-dimer to Tat affects both the chemical stability and the ability of the resulting construct to interact with and permeate the BBB.

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“…Beyond structural and morphological information commonly derived from imaging modalities, functional imaging further allows two- and three-dimensional visualization of physiological measures, including blood flow [1], tissue motion and deformation [2], neurological activity [3], diffusion and perfusion [4], and metabolism [5]. A well-known example is functional magnetic resonance imaging (fMRI), which indirectly determines brain activity through blood-oxygen-level dependent contrast [3, 6].…”

Section: Introductionmentioning

confidence: 99%

Functional imaging in OA: role of imaging in the evaluation of tissue biomechanics

Neu

2014

Osteoarthritis and Cartilage

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Functional imaging refers broadly to the visualization of organ or tissue physiology using medical image modalities. In load-bearing tissues of the body, including articular cartilage lining the bony ends of joints, changes in strain, stress, and material properties occur in osteoarthritis (OA), providing an opportunity to probe tissue function through the progression of the disease. Here, biomechanical measures in cartilage and related joint tissues are discussed as key imaging biomarkers in the evaluation of OA. Emphasis will be placed on the a) potential of radiography, ultrasound, and magnetic resonance imaging to assess early tissue pathomechanics in OA, b) relative utility of kinematic, structural, morphological, and biomechanical measures as functional imaging biomarkers, and c) improved diagnostic specificity through the combination of multiple imaging biomarkers with unique contrasts, including elastography and quantitative assessments of tissue biochemistry. In comparison to other modalities, magnetic resonance imaging provides an extensive range of functional measures at the tissue level, with conventional and emerging techniques available to potentially to assess the spectrum of preclinical to advance OA.

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Quantitative Cerebral Blood Flow Measurements Using MRI

Cited by 22 publications

References 18 publications

RETRACTED ARTICLE: Pericyte degeneration causes white matter dysfunction in the mouse central nervous system

RETRACTED ARTICLE: Pericyte degeneration causes white matter dysfunction in the mouse central nervous system

Conjugation of Therapeutic PSD-95 Inhibitors to the Cell-Penetrating Peptide Tat Affects Blood–Brain Barrier Adherence, Uptake, and Permeation

Functional imaging in OA: role of imaging in the evaluation of tissue biomechanics

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