Quantitative Changes in the Transcription of Phytohormone-Related Genes: Some Transcription Factors Are Major Causes of the Wheat Mutant dmc Not Tillering

He, Ruishi; Ni, Yongjing; Li, Junchang; Jiao, Zhixin; Zhu, Xin‐Xin; Jiang, Yumei; Li, Qiaoyun; Niu, Jishan

doi:10.3390/ijms19051324

Cited by 27 publications

(38 citation statements)

References 86 publications

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“…Reads distribution on 21 wheat chromosomes of T, M and D plants were analyzed and drawn using Circos tool software (http://mkweb.bcgsc.ca/tableviewer/). Gene functional annotation was carried out as previously described by us (He et al, 2018). Gene expression levels were estimated by fragments per kilobase of transcript per million fragments mapped (FPKM) (Florea, Song & Salzberg, 2013).…”

Section: Mrna Data Analysismentioning

confidence: 99%

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Peer Review #3 of "Cytological and molecular characterizations of a novel 2A nullisomic line derived from a widely-grown wheat cultivar Zhoumai 18 conferring male sterility (v0.1)"

Sharp¹

2020

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A dwarf, multi-pistil and male sterile dms mutant was previously reported by us. However, the genetic changes in this dms are unclear. To examine the genetic changes, single nucleotide polymorphism (SNP) association, chromosome counting, and high-resolution chromosome fluorescence in situ hybridization (FISH) techniques were employed. By comparing tall plants (T) with dwarf plants (D) in the offspring of dms mutant plants, SNP association analysis indicated that most SNPs were on chromosome 2A. There were 3 types in offspring of dms plants, with 42, 41 and 40 respectively. High-resolution chromosome painting analysis demonstrated that T plants had all 42 wheat chromosomes; the medium plants (M) had 41 chromosomes, lacking one chromosome 2A; while D plants had 40 wheat chromosomes, and lacked both 2A chromosomes. These data demonstrated that dms resulted from a loss of chromosome 2A. We identified 23 genes on chromosome 2A which might be involved in the development of stamens or pollen grains. These results lay a solid foundation for further analysis of the molecular mechanisms of wheat male sterility. Because D plants can be used as a female parent to cross with other wheat genotypes, dms is a unique germplasm for any functional study of chromosome 2A and wheat breeding specifically targeting genes on 2A.

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Section: Mrna Data Analysismentioning

confidence: 99%

“…The experimental samples were consistent with the samples of RNA-seq. The qRT-PCR was performed as previously described by us (He et al, 2018). Reverse transcription was performed with 1 μg RNA using Hifair ® Ⅱ 1st Strand cDNA Synthesis SuperMix (11123ES60, Yeasen, Shanghai, China).…”

Section: Qrt-pcr For Mrnasmentioning

confidence: 99%

Peer Review #3 of "Cytological and molecular characterizations of a novel 2A nullisomic line derived from a widely-grown wheat cultivar Zhoumai 18 conferring male sterility (v0.1)"

Sharp¹

2020

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“…Every sample was directly dissected in the expeimental field, and frozen immediately in liquid nitrogen. Total RNAs were extracted majorly in our report [88] using TRIZOL reagent (TransGen Biotech, Beijing, China).…”

Section: Sample Preparation and Rna Extraction For Rna-seqmentioning

confidence: 99%

“…(https://urgi.versailles.inra.fr/download/iwgsc/IWGSC_RefSeq_Assemblies/v1.0/). Gene functional annotation was carried out as described by He et al (2018) [88]. Gene expression levels were estimated by fragments per kilobase of transcript per million fragments mapped (FPKM) [89].…”

Section: Transcriptome Sequencing and Data Analysismentioning

confidence: 99%

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The Major Factors Causing the Microspore Abortion of Genic Male Sterile Mutant NWMS1 in Wheat (Triticum aestivum L.)

Zhang

et al. 2019

IJMS

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Male sterility is a valuable trait for genetic research and production application of wheat (Triticum aestivum L.). NWMS1, a novel typical genic male sterility mutant, was obtained from Shengnong 1, mutagenized with ethyl methane sulfonate (EMS). Microstructure and ultrastructure observations of the anthers and microspores indicated that the pollen abortion of NWMS1 started at the early uninucleate microspore stage. Pollen grain collapse, plasmolysis, and absent starch grains were the three typical characteristics of the abnormal microspores. The anther transcriptomes of NWMS1 and its wild type Shengnong 1 were compared at the early anther development stage, pollen mother cell meiotic stage, and binucleate microspore stage. Several biological pathways clearly involved in abnormal anther development were identified, including protein processing in endoplasmic reticulum, starch and sucrose metabolism, lipid metabolism, and plant hormone signal transduction. There were 20 key genes involved in the abnormal anther development, screened out by weighted gene co-expression network analysis (WGCNA), including SKP1B, BIP5, KCS11, ADH3, BGLU6, and TIFY10B. The results indicated that the defect in starch and sucrose metabolism was the most important factor causing male sterility in NWMS1. Based on the experimental data, a primary molecular regulation model of abnormal anther and pollen developments in mutant NWMS1 was established. These results laid a solid foundation for further research on the molecular mechanism of wheat male sterility.which is one of the key ways to improve wheat yield [6]. Male sterility permits the production of hybrids on a commercial scale, relying on heterosis in crops, and can greatly increase selection efficiency of the yield [7]. In-depth understanding of the molecular mechanism of male sterility can help us to use it more effectively.Plant male sterility was first reported in the 18th century [8]. A large number of plant male sterility mutants have been reported over the past few decades. Male sterility includes cytoplasmic male sterility (CMS) controlled by mitochondrial genes coupled with nuclear genes, and genic male sterility (GMS) controlled by nuclear genes alone [9][10][11]. The pistils of the cytoplasmic male sterile lines and genic male sterile lines are normal, but their stamens are abnormal and cannot pollinate normally, which is due to stamen degeneration, pollen abortion, or functional sterility. GMS occurs in 216 species and 17 species crosses, while CMS occurs in more than 150 species and 271 species crosses [11][12][13]. Plant male sterility lines have been widely studied and applied successfully in many crops, such as maize (Zea mays), rice (Oryza sativa), soybean (Glycine max), and barley (Hordeum vulgare). Several wheat CMS lines have been studied and applied in production; however, commercial application is limited [14][15][16]. Possible applications of GMS in plant breeding have been reviewed and discussed for at least 30 years [17]. Up to now, only five GMS genes have been...

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Comprehensive transcriptomics, proteomics, and metabolomics analyses of the mechanisms regulating tiller production in low-tillering wheat

Wang

Shi

et al. 2019

Theor Appl Genet

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Quantitative Changes in the Transcription of Phytohormone-Related Genes: Some Transcription Factors Are Major Causes of the Wheat Mutant dmc Not Tillering

Cited by 27 publications

References 86 publications

Peer Review #3 of "Cytological and molecular characterizations of a novel 2A nullisomic line derived from a widely-grown wheat cultivar Zhoumai 18 conferring male sterility (v0.1)"

Peer Review #3 of "Cytological and molecular characterizations of a novel 2A nullisomic line derived from a widely-grown wheat cultivar Zhoumai 18 conferring male sterility (v0.1)"

The Major Factors Causing the Microspore Abortion of Genic Male Sterile Mutant NWMS1 in Wheat (Triticum aestivum L.)

Comprehensive transcriptomics, proteomics, and metabolomics analyses of the mechanisms regulating tiller production in low-tillering wheat

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