Quantitative changes of brain isatin-binding proteins of rats with the rotenone-induced experimental parkinsonism

Buneeva, O. A.; Kapitsa, I. G.; Kazieva, L.Sh.; Vavilov, Nikita E.; Zgoda, Victor G.

doi:10.18097/pbmc20236903188

Cited by 5 publications

(2 citation statements)

References 14 publications

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“…Sample preparation for mass spectrometric analysis (protein extraction, alkylation and trypsinolysis) was carried out as described previously [9].…”

Section: Preparation Of Lysates Of Kidney Homogenatesmentioning

confidence: 99%

Comparative proteomic analysis of renal tissue of normotensive and hypertensive rats

Buneeva,

Fedchenko,

Kaloshina

et al. 2024

BIOMED KHIM

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Comparative proteomic analysis of kidney tissue from normotensive (WKY) and spontaneously hypertensive (SHR) rats revealed quantitative and qualitative changes in renal proteins. The number of renal proteins specific for WKY rats (blood pressure 110–120 mm Hg) was 13–16. There were 20–24 renal proteins specific for SHR (blood pressure 180 mm Hg and more). The total number of identified renal proteins common for both rat strains included 972–975 proteins. A pairwise comparison of all possible (SHR-WKY) variants identified 8 proteins specific only for normotensive (WKY) animals, and 7 proteins specific only for hypertensive ones (SHR). Taking into consideration their biological roles, the lack of some enzyme proteins in hypertensive rats (for example, biliverdin reductase A) reduces the production of molecules exhibiting antihypertensive properties, while the appearance of others (e.g. betaine-homocysteine S-methyltransferase 2, septin 2, etc.) can be interpreted as a compensatory reaction. Renal proteins with altered relative content (with more than 2.5-fold change) accounted for no more than 5% of all identified proteins. Among the proteins with an increased relative content in hypertensive animals, the largest group consisted of proteins involved in the processes of energy generation and carbohydrate metabolism, as well as antioxidant and protective proteins. In the context of the development of hypertension, the identified relative changes can apparently be considered compensatory. Among the proteins with the most pronounced decrease in the relative content in hypertensive rats, the dramatic reduction in acyl-CoA medium-chain synthetase-3 (ACSM3) appears to make an important contribution to the development of renal pathology in these animals.

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“…Sample preparation for mass spectrometric analysis (protein extraction, alkylation and trypsinolysis) was carried out as described previously [9].…”

Section: Preparation Of Lysates Of Kidney Homogenatesmentioning

confidence: 99%

Comparative proteomic analysis of renal tissue of normotensive and hypertensive rats

Buneeva,

Fedchenko,

Kaloshina

et al. 2024

BIOMED KHIM

Self Cite

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“…Sample preparation for mass spectrometric analysis (protein extraction, alkylation, and trypsinolysis) was carried out as described previously [20].…”

Section: Preparation Of Brain Homogenate Lysatesmentioning

confidence: 99%

Neuroprotective effects of isatin and afobazole in rats with rotenone-induced Parkinsonism are accompanied by increased brain levels of Triton X-100 soluble alpha-synuclein

Buneeva,

Kapitsa,

Zgoda

et al. 2023

BIOMED KHIM

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Effects of the endogenous neuroprotector isatin and the pharmacological drug afobazole (exhibiting neuroprotective properties) on behavioral reactions and quantitative changes in the brain proteomic profile have been investigated in rats with experimental rotenone Parkinsonism. A single dose of isatin (100 mg/kg subcutaneously on the last day of a 7-day course of rotenone administration) improved the motor activity of rats with rotenone-induced Parkinsonism in the open field test (horizontal movements) and the rotating rod test. Afobazole (10 mg/kg intraperitoneally, daily during the 7-day course of rotenone administration) reduced the manifestations of rigidity and postural instability. Proteomic analysis, performed using brain samples obtained the day after the last administration of rotenone and neuroprotectors, revealed similar quantitative changes in the brain of rats with rotenone Parkinsonism. An increase in the relative content of 65 proteins and a decrease in the relative content of 21 proteins were detected. The most pronounced changes — an almost ninety-fold increase in the alpha-synuclein content — were found in the brains of rats treated with isatin. In animals of the experimental groups treated with “Rotenone + Isatin”, as well as “Rotenone + Afobazole”, the increase in the relative content of this protein in the brain was almost 60 and 50 times higher than the control values. Taking into consideration the known data on the physiological role of alpha-synuclein, an increase in the content of this protein in the brain upon administration of neuroprotectors to animals with rotenone Parkinsonism may represent a compensatory reaction, at least in the early stages of this disease and the beginning of its treatment.

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Alterations in the plasma metabolome associated with maternal smoking during the first trimester

Masvosva,

Voutilainen,

Lehtonen

et al. 2024

Preprint

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Tobacco smoking during pregnancy has been associated with an increased risk of adverse outcomes like low birth weight. This study determined changes in the circulating metabolome linked to maternal smoking in the first trimester and correlated these changes to the growth of the fetus. The circulating metabolome was examined from first trimester plasma samples by non-targeted liquid chromatography mass spectrometry based metabolite profiling of 227 pregnant women (99 smokers and 117 non-smokers) from the Kuopio Birth Cohort Study. Tobacco smoking was self-reported through a questionnaire and verified with cotinine measurements from plasma samples. In summary, 64 significant differences were observed between the groups after correction for multiple testing e.g. in metabolites indicating endocrine disruption (e.g., dehydroepiandrosterone sulfate [DHEA-S], VIP=2.70, d=0.68, p<0.0001), metabolites associated with oxidative stress (e.g., bilirubin, VIP=2.00, d=0.50, p<0.0001) and lipid metabolism (e.g., LysoPC 16:1, VIP=2.07, d=0.51, p<0.0001). Some of these metabolites, e.g., DHEA-S and bilirubin, correlated with low birth weight, and some, e.g. LysoPC 16:1, correlated with small head circumference at birth. In conclusion, maternal smoking during the first trimester of pregnancy was associated with an altered metabolite profile linked to endocrine disruption and increased oxidative stress.

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Quantitative changes of brain isatin-binding proteins of rats with the rotenone-induced experimental parkinsonism

Cited by 5 publications

References 14 publications

Comparative proteomic analysis of renal tissue of normotensive and hypertensive rats

Comparative proteomic analysis of renal tissue of normotensive and hypertensive rats

Neuroprotective effects of isatin and afobazole in rats with rotenone-induced Parkinsonism are accompanied by increased brain levels of Triton X-100 soluble alpha-synuclein

Alterations in the plasma metabolome associated with maternal smoking during the first trimester

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