Quantitative characterization of glutaminolysis in human plasma using liquid chromatography-tandem mass spectrometry

Hua, Yi-Jun; Yang, Xuping; Li, Ruiting; Liu, Peifang; Liu, Peijia; Li, Linrui; Yuan, Xia; Hua, Xiaoyi; Tian, Yuan; Zhang, Zunjian; Huang, Yin

doi:10.1007/s00216-019-01626-3

Cited by 5 publications

(2 citation statements)

References 27 publications

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“…Tricarboxylic acid (TCA) intermediates and relevant metabolites were measured by LC‐MS/MS as described previously 29 . TCA cycle pool size was measured by the sum of seven TCA intermediates concentration, namely, citrate, iso‐citrate, cis‐aconitate, α‐ketoglutarate, succinate, fumarate, and malate.…”

Section: Methodsmentioning

confidence: 99%

Time‐restricted feeding alleviates cardiac dysfunction induced by simulated microgravity via restoring cardiac FGF21 signaling

et al. 2020

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Dietary restriction has been well-described to improve health metrics, but whether it could benefit pathophysiological adaptation to extreme environment, for example, microgravity, remains unknown. Here, we investigated the effects of a daily rhythm of fasting and feeding without reducing caloric intake on cardiac function and metabolism against simulated microgravity. Male rats under ad libitum feeding or time-restricted feeding (TRF; food access limited to 8 hours every day) were subjected to hindlimb unloading (HU) to simulate microgravity. HU for 6 weeks led to left ventricular dyssynchrony and declined cardiac function. HU also lowered pyruvate dehydrogenase (PDH) activity and impaired glucose utilization in the heart. All these were largely preserved by TRF. TRF showed no effects on HU-induced loss of cardiac mass, but significantly improved contractile function of cardiomyocytes. Interestingly, TRF raised liver-derived fibroblast growth factor 21 (FGF21) level and enhanced cardiac FGF21 signaling as manifested by upregulation of FGF receptor-1 (FGFR1) expression and its downstream markers in HU rats. In isolated cardiomyocytes, FGF21 treatment improved PDH activity and glucose utilization, consequently enhancing cell contractile function. Finally, both liver-specific knockdown (KD) of FGF21 and cardiac-specific FGFR1 KD abrogated the cardioprotective effects of TRF in HU rats. These data demonstrate that TRF improves cardiac

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Section: Methodsmentioning

confidence: 99%

Time‐restricted feeding alleviates cardiac dysfunction induced by simulated microgravity via restoring cardiac FGF21 signaling

et al. 2020

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“…The metabolomics analyses, including sample preparation and LC/GC-MS data acquisition, are performed according to our previous study with some modification [19,20].…”

Section: Metabolomics Analysis and Data Processingmentioning

confidence: 99%

Metabolic network-based identification of plasma markers for non-small cell lung cancer

Guo

Zhao

et al. 2021

Anal Bioanal Chem

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Metabolic markers, offering sensitive information on biological dysfunction, play important roles in diagnosing and treating cancers. However, the discovery of effective markers is limited by the lack of well-established metabolite selection approaches. Here, we propose a network-based strategy to uncover the metabolic markers with potential clinical availability for non-small cell lung cancer (NSCLC). First, an integrated mass spectrometry-based untargeted metabolomics was used to profile the plasma samples from 43 NSCLC patients and 43 healthy controls. We found that a series of 39 metabolites were altered significantly. Relying on the human metabolic network assembled from Kyoto Encyclopedia of Genes and Genomes (KEGG) database, we mapped these differential metabolites to the network and constructed an NSCLC-related disease module containing 23 putative metabolic markers. By measuring the PageRank centrality of molecules in this module, we computationally evaluated the network-based importance of the 23 metabolites and demonstrated that the metabolism pathways of aromatic amino acids and long-chain fatty acids provided potential molecular targets of NSCLC (i.e., IL4l1 and ACOT2). Combining network-based ranking and support-vector machine modeling, we further found a panel of eight metabolites (i.e., pyruvate, tryptophan, and palmitic acid) that showed a high capability to differentiate patients from controls (accuracy > 97.7%). In summary, we present a meaningful network method for metabolic marker discovery and have identified eight strong candidate metabolites for NSCLC diagnosis.

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Analytical perspective on biomarkers research: from untargeted to targeted metabolomics

Nemutlu

Eylem

Reçber

et al. 2022

The Detection of Biomarkers

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Quantitative characterization of glutaminolysis in human plasma using liquid chromatography-tandem mass spectrometry

Cited by 5 publications

References 27 publications

Time‐restricted feeding alleviates cardiac dysfunction induced by simulated microgravity via restoring cardiac FGF21 signaling

Time‐restricted feeding alleviates cardiac dysfunction induced by simulated microgravity via restoring cardiac FGF21 signaling

Metabolic network-based identification of plasma markers for non-small cell lung cancer

Analytical perspective on biomarkers research: from untargeted to targeted metabolomics

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