Quantitative Chemical Composition of Peptidoglycan of
            <i>Listeria monocytogenes</i>

Srivastava, K; Siddique, I. H.

doi:10.1128/iai.7.5.700-703.1973

Cited by 14 publications

(6 citation statements)

References 13 publications

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“…The TEICH fractions yielded the highest values, presumably reflecting ribitol or glycerol and attached glucose or galactose residues. These carbohydrate values agree with those previously reported (10,18,19).…”

Section: Discussionsupporting

confidence: 93%

Immunopotentiating activities of cell walls, peptidoglycans, and teichoic acids from two strains of Listeria monocytogenes

Paquet¹,

Raines²,

Brownback³

1986

Infect Immun

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Crude cell wall (CCW), enzyme purified wall (PF), peptidoglycans (PEP), and teichoic acid fractions from two strains of Listeria monocytogenes (EGD and a human isolate) were analyzed by electron microscopy, chemical analyses, and various immunological assays. Electron microscopy and chemical analyses revealed that both strains were quite similar. The CCW, PF, and PEP fractions were mitogenic for mouse lymphocytes, could activate macrophages in vivo but not in vitro, and stimulated nonspecific immunity in vivo to Candida albicans. The PF and PEP fractions but not the CCW induced migration inhibition factor production. The CCW and PEP fractions were tested and found to elevate natural killer activity in vivo. Teichoic acid fractions had no activity in the above assays. Since most of the immunological activities were retained in the PEP fraction, we examined this fraction to determine the kinetics of the mitogenic response and the nature of the responding cell population. The peak mitogenic response occurred on day 2 over a 6-day period. This response was not enriched in T-cell-enriched populations but followed the pattern of LPS in B-cell-enriched populations. * Corresponding author. 170 sterile wire screen into 5 ml of cold tissue culture medium.

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Section: Discussionsupporting

confidence: 93%

Immunopotentiating activities of cell walls, peptidoglycans, and teichoic acids from two strains of Listeria monocytogenes

Paquet¹,

Raines²,

Brownback³

1986

Infect Immun

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“…Ribitol was found in LCWF and decreased considerably in the preparation of PF and BHPF. Ribitol is a common constituent of teichoic acids, although teichoic acids were not detected in an analysis of serotype 4b cells (30). Ribitol has not been previously recognized in Listeria cell walls.…”

Section: Discussionmentioning

confidence: 72%

“…Glucosamine, glucose, and, most importantly, rhamnose are known to be serologically active sugars for L. monocytogenes serotype 1 (32). High quantities of rhamnose are also present in serotype 4b cells (30). No monosaccharides with molecular weights higher than those of hexoses or hexosamines were detected in any of the cell wall hydrolysates.…”

Section: Discussionmentioning

confidence: 99%

Chemical composition and biological functions of Listeria monocytogenes cell wall preparations

Hether¹,

Campbell²,

Baker³

et al. 1983

Infect Immun

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A crude Listeria cell wall fraction, a purified fraction (PF) with demonstrated biological activity, as well as a third fraction of base-hydrolyzed PF (BHPF) were analyzed for chemical composition and activities not previously described. Listeria cell wall fraction and PF contained significant quantities of lipid, whereas BHPF was lipid depleted. Fatty acid compositions were typical of gram-positive bacteria. PF and BHPF were depleted in protein. Alanine, glutamic acid, diaminopimelic acid, glucosamine, and muramic acid were found in all fractions, in enhanced concentration in PF and BHPF, and with molar ratios typical of bacterial peptidoglycans. Major neutral sugars were rhamnose, ribose, ribitol, and glucose. The concentrations of rhamnose, ribose, and glucose were increased in BHPF. Differences in chemical composition of the fractions reflected differences in their biological activities: Listeria cell wall fraction induced resistance to Listeria infection, whereas PF did not. Mitogenic and adjuvant activities were demonstrated for Listeria cell wall fraction and PF but were lost in BHPF. BHPF retained the ability to induce macrophage-mediated tumoricidal activity and decrease resistance to Listeria infection.

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“…33 This model is useful for hydrophilic polysaccharides 69 and proteins 70 which are the major components of bacterial surfaces. 71 The contact angle (θ) is related to γ i LW , the apolar part of surface tension of condensed material (i) caused by dispersion energy between molecules, and to γ i P , the polar part of surface tension of condensed material (i) caused by dipole interaction included dipole moments and hydrogen bonds according to…”

Section: ' Mathematical Modelingmentioning

confidence: 99%

The Role of Growth Temperature in the Adhesion and Mechanics of Pathogenic L. monocytogenes: An AFM Study

Gordesli

Abu-Lail

2011

Langmuir

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The adhesion strengths of pathogenic L. monocytogenes EGDe to a model surface of silicon nitride were quantified using atomic force microscopy (AFM) in water for cells grown under five different temperatures (10, 20, 30, 37, and 40 °C). The temperature range investigated was chosen to bracket the thermal conditions in which L. monocytogenes survive in the environment. Our results indicated that adhesion force and energy quantified were at their maximum when the bacteria were grown at 30 °C. The higher adhesion observed at 30 °C compared to the adhesion quantified for bacterial cells grown at 37, 40, 20, and 10 °C was associated with longer and denser bacterial surface biopolymer brushes as predicted from fitting a model of steric repulsion to the approach distance-force data as well from the results of protein colorimetric assays. Theoretically predicted adhesion energies based on soft-particle DLVO theory agreed well with the adhesion energies computed from AFM force-distance retraction data (r(2) = 0.94); showing a minimum energy barrier to adhesion at 30 °C.

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Quantitative Chemical Composition of Peptidoglycan of Listeria monocytogenes

Cited by 14 publications

References 13 publications

Immunopotentiating activities of cell walls, peptidoglycans, and teichoic acids from two strains of Listeria monocytogenes

Immunopotentiating activities of cell walls, peptidoglycans, and teichoic acids from two strains of Listeria monocytogenes

Chemical composition and biological functions of Listeria monocytogenes cell wall preparations

The Role of Growth Temperature in the Adhesion and Mechanics of Pathogenic L. monocytogenes: An AFM Study

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