Quantitative comparative phosphoproteomic analysis of the effects of colostrum and milk feeding on liver tissue of neonatal calves

Abstract

Posttranslational modifications, mostly phosphorylation, are critical for protein structure and function. However, the association between liver phosphoproteins in neonatal calves and colostrum intake is not well understood. In this study, we examined the liver phosphoproteome profile in neonatal calves after receiving colostrum or milk. Liver tissue samples were collected from control calves (CON, n = 3) 2 h after birth and from calves that received colostrum (CG, n = 3) or milk (MG, n = 3) 24 h after birth. Hepatic phosphoprotein expression profiles were analyzed using quantitative proteomics based on the liquid chromatography-tandem mass spectrometry method. In total, 1,587 phosphorylated sites were identified in 1,011 liver proteins. The most abundant phosphorylation site AA was serine (87.5%), followed by threonine (11.9%) and tyrosine (0.5%). Among the 1,011 phosphoproteins, 219, 453, and 26 displayed differential expression in the CG versus MG, CG versus CON, and MG versus CON comparisons, respectively. Differentially expressed phosphoproteins in the CG-MG comparison included 3-phosphoinositide-dependent protein kinase 1, glucose transporter member 4, protein kinase N2, and vinculin, which were mainly involved in the glycogen metabolic process, transport, growth and development, and cell adhesion process, according to Gene Ontology analysis. Pathway analysis indicated their enrichment in the insulin signaling pathway, spliceosome, and adherens junction. The CG-CON comparison identified differentially expressed phosphoproteins and their target genes that were largely involved in the cellular process, macromolecule metabolic process, developmental process, and transport. Pathway analysis indicated their association with endocytosis, mechanistic target of rapamycin, AMP-activated protein kinase, and insulin signaling pathways. These data demonstrate that changes in the phosphoproteins of liver tissues may play an important role in energy metabolism and immune response in the calves that received colostrum. These results provide novel insights into the crucial roles of protein phosphorylation during the early life of newborn calves.

Effects of alkaline mineral complex supplementation on production performance, serum variables, and liver transcriptome in calves

“…After slaughtering, liver samples were collected from the same areas of calves in the C and T groups. Liver sample collection was done according to the method of Zhao et al ( 31 ). Briefly, liver samples were quickly sectioned, washed with precooled PBS (4°C), placed in 2 mL sterile cryopreservation tubes, quickly frozen in liquid nitrogen, and then stored at −80°C for further analysis.…”

Yeast Culture Supplementation Effects on Systemic and Polymorphonuclear Leukocytes’ mRNA Biomarkers of Inflammation and Liver Function in Peripartal Dairy Cows

“…Regarding VCL, it encodes a protein in the cell as soluble, cytoplasmic, and membrane-bound, which is involved in cell functions such as adhesion, motility, and spreading [48,49]. Moreover, VCL has been associated with immune function, reducing the susceptibility to virus infections [50,51]. Overall, those results might indicate that peripartal YC supplementation affects neutrophil activation and function.…”