Quantitative comparison of cancer and normal cell adhesion using organosilane monolayer templates: an experimental study on the anti-adhesion effect of green-tea catechins

Abstract

The main constituent of green tea, (-)-Epigallocatechin-3-O-gallate (EGCG), is known to have cancer-specific chemopreventive effects. In the present work, we investigated how EGCG suppresses cell adhesion by comparing the adhesion of human pancreatic cancer cells (AsPC-1 and BxPC-3) and their counterpart, normal human embryonic pancreas-derived cells (1C3D3), in catechin-containing media using organosilane monolayer templates (OMTs). The purpose of this work is (1) to evaluate the quantitativeness in the measurement of cell adhesion with the OMT and (2) to show how green-tea catechins suppress cell adhesion in a cancer-specific manner. For the first purpose, the adhesion of cancer and normal cells was compared using the OMT. The cell adhesion in different type of catechins such as EGCG, (-)-Epicatechin-3-O-gallate (ECG) and (-)-Epicatechin (EC) was also evaluated. The measurements revealed that the anti-adhesion effect of green-tea catechins is cancer-specific, and the order is EGCG≫ECG>EC. The results agree well with the data reported to date, showing the quantitativeness of the new method. For the second purpose, the contact area of cells on the OMT was measured by reflection interference contrast microscopy. The cell-OMT contact area of cancer cells decreases with increasing EGCG concentration, whereas that of normal cells remains constant. The results reveal a twofold action of EGCG on cancer cell adhesion-suppressing cell attachment to a candidate adhesion site and decreasing the contact area of the cells-and validates the use of OMT as a tool for screening cancer cell adhesion.

Effect of trypsin concentration on living SMCC‐7721 cells studied by atomic force microscopy

“…Cell adhesion is crucial for cell transfer, shape maintenance and mechanical signal generation. It is also the basis of the physiological and pathological processes such as wound healing, recombination of neurons and cancer metastasis 31,32 . Trypsin can hydrolyse proteins on the cell surface, which in turn affects cell surface adhesion.…”

In situ modification of cell-culture scaffolds by photocatalysis of visible-light-responsive TiO2film

“…The surface micropattern defines the cell-permissive and nonpermissive areas with a subcellular resolution and regulates cellular processes both spatially and temporally, providing a novel platform for cell analyses. The identification of the cell type 6,7) and the quantitative comparison of its functions [8][9][10] have been demonstrated on micropatterned scaffolds designed specifically for the functions. Currently, such micropatterning can be performed not only in air but also in a medium where cells are being cultured.…”