Quantitative comparison of PTH1R in breast cancer MCF7 and osteosarcoma SaOS‐2 cell lines

Alokail, Majed S.; Peddie, M.J.

doi:10.1002/cbf.1475

Cited by 8 publications

(5 citation statements)

References 47 publications

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“…Consequently, a cAMP immunoassay showed activation of the cAMP/PKA pathway through human N‐terminal PTH, N‐terminal PTHrP, but not through the C‐terminal PTHrP fragment. These results are in accordance with recent publications which show that breast cancer (MCF7) and osteosarcoma cells (Saos‐2) frequently express PTH1R 20, 21…”

Section: Resultssupporting

confidence: 93%

Biliary intraepithelial neoplasia in patients without chronic biliary disease

Levy

Correa

et al. 2009

Cancer

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Absolute counting of total leukocytes or specific subsets within small amounts of whole blood is difficult due to a lack of techniques that enable separation of all leukocytes from limited amounts of whole blood. In this study, a microfluidic device equipped with a size‐controlled microcavity array for highly efficient separation of leukocytes from submicroliters of whole blood was developed. The microcavity array can separate leukocytes from whole blood based on differences in the size and deformability between leukocytes and other blood cells. Leukocytes recovered on aligned microcavities were continuously processed for image‐based immunophenotypic analysis. Our device successfully recovered over 90% of leukocytes in 1 µL of whole blood without pretreatment such as density gradient centrifugation or erythrocyte lysis. In addition, the proposed system successfully performed absolute enumeration of human CD4+ and CD8+ leukocytes from 1 µL of whole blood, and the obtained data showed good correlation with conventional flow cytometric analysis. Our microfluidic device has great potential as a tool for a point‐of‐care leukocyte analysis system. Biotechnol. Bioeng. 2012; 109:2017–2024. © 2012 Wiley Periodicals, Inc.

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Section: Resultssupporting

confidence: 93%

Biliary intraepithelial neoplasia in patients without chronic biliary disease

Levy

Correa

et al. 2009

Cancer

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“…These results are in accordance with recent publications which show that breast cancer (MCF7) and osteosarcoma cells (Saos-2) frequently express PTH1R. 20,21 Both PTHrP fragments, N-and C-terminal PTHrP, significantly inhibited apoptosis of Saos-2 cells treated with chemotherapeutic drugs. N-terminal PTHrP inhibited doxorubicin-, mitoxantroneand bleomycin-induced apoptosis, whereas C-terminal PTHrP repressed doxorubicin-and bleomycin-induced apoptosis but not mitoxantrone-induced apoptosis (Fig.…”

Section: Pthrp Inhibits Apoptosis Induced By Chemotherapeutic Agentssupporting

confidence: 92%

Parathyroid hormone‐related protein confers chemoresistance by blocking apoptosis signaling via death receptors and mitochondria

Gagiannis

Müller

Uhlemann

et al. 2009

Intl Journal of Cancer

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Parathyroid hormone (PTH) has a central role in the regulation of serum calcium and phosphate, whereas parathyroid hormonerelated peptide (PTHrP) has important developmental roles. In addition, PTHrP has been discovered as a causative agent of hypercalcemia of malignancy. PTHrP is also expressed in many tumors, and expression often correlates with unfavorable prognosis. We have investigated the effects of PTHrP on apoptosis signaling pathways initiated by DNA damaging chemotherapeutic drugs. Stimulation experiments of the CD95-, the TNF-R-, and the TRAIL-R-death receptor systems in Saos human osteosarcoma cells revealed that PTHrP can block signaling via each of these death receptors. Furthermore, our findings demonstrate a link between PTHrP and the mitochondrial apoptosis pathway. PTHrP down-regulates expression of pro-apoptotic Bcl-2 family members like Bax and PUMA and up-regulates expression of antiapoptotic molecules like Bcl-2 and Bcl-xl. It is of clinical relevance that PTHrP and anticancer drugs show opposing interactions on death receptor-triggered as well as on mitochondrial apoptosis pathways. In addition, PTHrP induces chemoresistance by interference with p53 family-dependent apoptosis signaling pathways and p53-mediated transactivation of apoptosis target genes. Inhibition of CD95-and Bax gene transactivation is a mechanism by which PTHrP reduced the apoptosis response and treatment sensitivity of tumor cells. Our data indicate that PTHrP inhibits major apoptosis signaling pathways by blocking signaling via p53, death receptors and mitochondria and, consequently, confers chemoresistance of cancer cells. Thus, beyond its importance in development and differentiation, we describe an important role for PTHrP in tumorigenesis. ' UICCKey words: apoptosis; chemosensitivity; p53 family; PTHrP; PTH Parathyroid hormone-related peptide (PTHrP) is a 17-kDa peptide hormone that shares 70% sequence homology with parathyroid hormone (PTH) over the first 13 amino acids at the N-terminus. Both, PTH and PTHrP signal through a common PTH/ PTHrP receptor (a class B G-protein-coupled receptor) and share similar biological activities. PTH is a circulating hormone composed of 84 amino acids. It is produced in the parathyroid glands and acts primarily on bone and kidney to maintain extracellular calcium levels within a normal range.

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“…It cooperates with extracellular calcium sensitive receptors (CaSR) to maintain the physiological homeostasis of extracellular calcium ions (Ca2 + ) and lactation. It can promote the proliferation of breast cancer and is closely related to the bone metastasis of breast cancer ( Alokail and Peddie 2008 ; Dittmer et al, 2006 ; Liang et al, 2012 ; Yang and Wang 2018 ). Some studies in GC have shown that PTH1R can be expressed in some gastric cancer cell lines, and reduced PTH1R expression can promote gastric hypergastrinemia and gastric neuroendocrine tumors ( Al Menhali et al, 2017 ; Calvete et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Immune Infiltration in Gastric Cancer Microenvironment and Its Clinical Significance

Zhang

Yuan

Liang

et al. 2022

Front. Cell Dev. Biol.

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Immunotherapy has developed rapidly and has gradually become one of the important methods for treatment of gastric cancer (GC). The research on tumor infiltrating immune cells (TIICs) and immune-related genes in the tumor microenvironment (TME) greatly encourages the development of immunotherapy. The devolution algorithm (CIBERSORT) was applied to infer the proportion of 22 TIICs based on gene expression profiles of GC tissues, which were downloaded from TCGA and GEO. TCGA was utilized to analyze the differential expression of immune-related genes, and explore the potential molecular functions of these genes. We have observed the enrichment of multiple TIICs in microenvironment of GC. Some of these cells were closely related to tumor mutational burden (TMB), microsatellite instability (MSI), Fuhrman grade, and TNM staging. Survival analysis showed that the infiltration level of CD8+ T cells, activated CD4+ memory T cells and M2 macrophages were significantly related to the prognosis of GC patients. The functional enrichment analysis of immune-related genes revealed that these genes were mainly associated with cytokine activation and response. Four significant modules were screened by PPI network and 20 key genes were screened from the modules. The expression levels of CALCR and PTH1R are strikingly related to the expression of immune checkpoint and the prognosis of GC patients. The type and number of TIICs in microenvironment of GC, as well as immune-related genes are closely related to tumor progression, and can be used as important indicators for patient prognosis assessment.

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Quantitative comparison of PTH1R in breast cancer MCF7 and osteosarcoma SaOS‐2 cell lines

Cited by 8 publications

References 47 publications

Biliary intraepithelial neoplasia in patients without chronic biliary disease

Biliary intraepithelial neoplasia in patients without chronic biliary disease

Parathyroid hormone‐related protein confers chemoresistance by blocking apoptosis signaling via death receptors and mitochondria

Immune Infiltration in Gastric Cancer Microenvironment and Its Clinical Significance

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