Quantitative Counterimmunoeiectrophoresis for Urinary Fibrinogen-related Antigens

A laser nephelometric procedure to quantitate fibrinogen related antigens (FRA), that joined the best characteristics of the immunological available techniques, is proposed. It is sensitive, rapid, simple, reproducible, antiserum saving and accurate since it correlates very well with the tanned red cell hemagglutination inhibition immunoassay (TRCHII) at the most commonly found clinical levels (r = 0.98, p less than 0.0005), but with the advantage of a more objective quantitation and thus, reliable for any concentration of FRA. Prepared samples of different known levels of fibrinogen, assayed with the laser method and the TRCHII, showed a higher correlation with the laser procedure (r = 0.97, p less than 0.0005) than the obtained with the TRCHII (r = 0.85, p less than 0.001). The technic is useful in emergency situations, routine work and research.

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Laser Nephelometry Quantitation of Fibrinogen-related Antigens

Ruiz-Arenas

Lepe-Zuniga

Ruı́z-Argüelles

et al. 1981

Am J Clin Pathol

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Counterimmunoelectrophoresis in Biology and Medicine

Tilton

1978

CRC Critical Reviews in Clinical Laboratory Sciences

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The review will initially focus on the historical development of counterimmunoelectrophoresis (CIE). A part of the manuscript will be devoted to methodological considerations of CIE as well as a discussion of the antisera used. The body of the review will detail each test procedure for which CIE is applicable and discuss unique elements of technique and limits of detection, as well as clinical interpretation of results. The final section will summarize potential applications of CIE in microbiology.

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Quantitative Counterimmunoeiectrophoresis for Urinary Fibrinogen-related Antigens

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Laser Nephelometry Quantitation of Fibrinogen-related Antigens

Laser Nephelometry Quantitation of Fibrinogen-related Antigens

Counterimmunoelectrophoresis in Biology and Medicine

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