Quantitative description of classic and variant small cell lung cancer cell lines by nuclear image cytometry

Abstract

Six small cell lung cancer (SCLC) cell lines were examined using nuclear image analysis to find features characteristic of the classic and the variant type of SCLC. On the basis of their biochemical and biological properties three of these cell lines have been shown to represent the classic types, and three represent the variant type of SCLC. Using a combination of the image‐derived run length, density, and geometric features, it was possible to distinguish between the classic and variant SCLC cell lines.The results of this study may be of help in assessing photometric features for the separation of the classic and variant subtypes of SCLC in solid tumors. Because of differences in treatment and prognosis between these two subtypes, such a separation may be of clinical value.

Acetylcholine signaling system in progression of lung cancers

“…H82 is a variant human SCLC cell line, whereas H69 is a classical human SCLC cell line. The variant SCLC cells are associated with accelerated doubling time, greater invasive phenotype and lower sensitivity to growth-inhibitory agents than classical SCLC cell lines like H69 (Broers, et al, 1988;Gazdar, Carney, Nau, & Minna, 1985). This may explain the lower growth-suppressive activity of CTL1-siRNA in H82 relative to H69 human SCLC cells.…”

Hypothesis: a novel route for immortalization of epithelial cells by Epstein-Barr virus

“…This procedure was performed as described (Broers et al, 1986), using mouse monoclonal antibodies 6B10 (keratin 4), LP1K (keratin 7), LE41 (keratin 8), LH2 (keratin 10), IC7 (keratin 13), LL001 (keratin 14), LE61 (keratin 18), LP2K (keratin 19), and LP34 (pancytokeratin) (all gifts from Dr S Chang). Once established, the parotid line (M845PE) stained positive with LP34 and was further typed using the monoclonal antibodies to simple, basal and non-corni®ed, strati®ed or squamous epithelium.…”

Distinction of two different classes of small-cell lung cancer cell lines by enzymatically inactive neuron-specific enolase

“…However the presence of NSE in many non-neuroendocrine tissues has raised questions on the specificity of NSE. We have investigated NSE immunoreactivity (NSA-ag), y-enolase activity and total enolase activity in small cell lung cancer (SCLC) (Bepler et al, 1987;Carney et al, 1985;Gazdar et al, 1985;Bepler et al, 1989a;Broers et al, 1985;Moody et al, 1985;Broers et al, 1988 NSE is widely used as a neuroendocrine marker. NSEimmuno reactivity is not only seen in neurons, but also in neuroendocrine cells present in endocrine glands and in the diffuse neuroendocrine systems of the lung, intestine, thymus and skin.…”

“…variant and classic cell-lines, characterised by the absence or presence respectively of the enzyme L-dopadecarboxylase. In comparison with classic cell lines variant cell lines were shown to have a higher growth rate, a higher cloning efficiency, a larger cell volume, a lower content of Neuron-Specific Enolase (NSE), amplification of c-myc, absence of gastrin releasing peptide (GRP) and neurotensin, and a decreased sensitivity to radiotherapy and chemotherapy (Bepler et al, 1987;Carney et al, 1985;Gazdar et al, 1985;Bepler et al, 1989a;Broers et al, 1985;Moody et al, 1985;Broers et al, 1988). Recently Bepler et al (1989b) added a third class, so-called transitional cells, based on the presence of p64 c-myc in some of the classic cell lines.…”