Quantitative Detection of Allergenic Protein <i>Sin a</i> 1 from Yellow Mustard (Sinapis alba L.) Seeds Using Enzyme-Linked Immunosorbent Assay

Shim, Youn-Young; Wanasundara, Janitha P. D.

doi:10.1021/jf072660u

Cited by 28 publications

(12 citation statements)

References 23 publications

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“…Aluko and McIntosh (2004) demonstrated that 12 and 13 kDa polypeptides were subunits of the napin of mustard seed. Shim and Wanasundara (2008) reported that a single protein band of 14.5 kDa polypeptides were two polypeptide chains of 4.5 and 10 kDa linked by disulfide bonds. From the above information, it was concluded that the bands found in SDS-PAGE were cruciferin and napin, and that they could be extracted with either TS or NaCl solution.…”

Section: Discussionmentioning

confidence: 99%

Biorefinery process for protein extraction from oriental mustard (Brassica juncea (L.) Czern.) using ethanol stillage

et al. 2012

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Large volumes of treated process water are required for protein extraction. Evaporation of this water contributes greatly to the energy consumed in enriching protein products. Thin stillage remaining from ethanol production is available in large volumes and may be suitable for extracting protein rich materials. In this work protein was extracted from ground defatted oriental mustard (Brassica juncea (L.) Czern.) meal using thin stillage. Protein extraction efficiency was studied at pHs between 7.6 and 10.4 and salt concentrations between 3.4 × 10-2 and 1.2 M. The optimum extraction efficiency was pH 10.0 and 1.0 M NaCl. Napin and cruciferin were the most prevalent proteins in the isolate. The isolate exhibited high in vitro digestibility (74.9 ± 0.80%) and lysine content (5.2 ± 0.2 g/100 g of protein). No differences in the efficiency of extraction, SDS-PAGE profile, digestibility, lysine availability, or amino acid composition were observed between protein extracted with thin stillage and that extracted with NaCl solution. The use of thin stillage, in lieu of water, for protein extraction would decrease the energy requirements and waste disposal costs of the protein isolation and biofuel production processes.

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Section: Discussionmentioning

confidence: 99%

Biorefinery process for protein extraction from oriental mustard (Brassica juncea (L.) Czern.) using ethanol stillage

et al. 2012

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“…Quantitative detection of mustard allergens in food can be accomplished by sandwich-type ELISA with LODs as low as 1 μ g of ground whole mustard seeds per mL [133, 134] or qPCR [111]. …”

Section: Food Ingredients Allergenic Fractions and Recognized Almentioning

confidence: 99%

Food Production and Processing Considerations of Allergenic Food Ingredients: A Review

Álvarez

Boye

2012

Journal of Allergy

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Although most consumers show no adverse symptoms to food allergens, health consequences for sensitized individuals can be very serious. As a result, the Codex General Standard for the Labelling of Prepackaged Foods has specified a series of allergenic ingredients/substances requiring mandatory declaration when present in processed prepackaged food products. Countries adhering to international standards are required to observe this minimum of eight substances, but additional priority allergens are included in the list in some countries. Enforcement agencies have traditionally focused their effort on surveillance of prepackaged goods, but there is a growing need to apply a bottom-up approach to allergen risk management in food manufacturing starting from primary food processing operations in order to minimize the possibility of allergen contamination in finished products. The present paper aims to review food production considerations that impact allergen risk management, and it is directed mainly to food manufacturers and policy makers. Furthermore, a series of food ingredients and the allergenic fractions identified from them, as well as the current methodology used for detection of these allergenic foods, is provided.

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“…The ELISA method is by far the most common and is routinely employed in various food analysis labs due to its high precision, simple handling and good potential for standardization. Additionally, quantitative data are possible with the ELISA technique (Shim and Wanasundara, 2008). However results generated with the ELISA method must be sometimes taken with caution as substantial differences in the detectable protein from the standard on which the test is based, resulting for example from variations in the processing of the food matrix, might lead to false results.…”

Section: Pcr-based Allergen Detection and Quantification In Food Matrmentioning

confidence: 99%

The Application of PCR-Based Methods in Food Control Agencies – A Review

Iwobi¹,

Huber²,

Busch³

2012

Polymerase Chain Reaction

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Quantitative Detection of Allergenic Protein Sin a 1 from Yellow Mustard (Sinapis alba L.) Seeds Using Enzyme-Linked Immunosorbent Assay

Cited by 28 publications

References 23 publications

Biorefinery process for protein extraction from oriental mustard (Brassica juncea (L.) Czern.) using ethanol stillage

Biorefinery process for protein extraction from oriental mustard (Brassica juncea (L.) Czern.) using ethanol stillage

Food Production and Processing Considerations of Allergenic Food Ingredients: A Review

The Application of PCR-Based Methods in Food Control Agencies – A Review

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