Quantitative detection of cartilage surfaces and ligament geometry of the wrist using an imaging cryomicrotome system

The effect of lunate position on range of motion after a four-corner arthrodesis: A biomechanical simulation study

“…Anatomical data, acquired from cryomicrotome images of the human wrist, were used as geometric input of the model (Dvinskikh et al, 2010). In the model, the carpal bones or group of carpal bones were modelled as rigid bodies, connected to each other through non-linear links: articular contacts and ligaments.…”

“…K ¼ L 0 k is the stiffness of the spring, where k is the ligament stiffness parameter and 2e L is the linear strain limit (Wismans et al, 1980). Based on specimen specific carpal bone kinematics and anatomical data of the ligament insertions, the zero-load lengths (L 0 ) of the ligaments were estimated and used in the model (Dvinskikh et al, 2010;Carelsen et al, 2009). For each ligament line element, the maximal length Lmax occurring at least once for the ranges of flexion-extension and ulnar-radial deviation was determined and zeroload ligament length was calculated using the method described by Blankevoort et al (1991b):…”

Osseous and Ligamentous Scaphoid Anatomy: Part II. Evaluation of Ligament Morphology Using Three-Dimensional Anatomical Imaging

“…6,7 The stack of consecutive 2-dimensional images builds up a 3-dimensional image of each test specimen. Before imaging the cadaver specimen, the cryomicrotome system was calibrated by using a calibration object so that the pixel size of the digital image was 100 ϫ 100 m 2 .…”

“…The ligaments and tendons were imaged by using the autofluorescence properties of collagen type I that is present in these anatomical structures, as the cryomicrotome imaging system allows for simultaneous acquisition in white light and fluorescence mode. 6,7 The imaging system also detects collagen present in the bone. However, the 3-dimensional imaging system made it possible to distinguish between collagen in bone and outside bone based on location and appearance because the ligaments appear in fiber structures.…”

“…Such an accurate quantitative detection of cartilage surfaces and ligament geometry of the wrist using this methodology has recently been reported and has been reproduced in the current study. 7 The primary aim of this study was to provide both a more accurate and a quantitative description of the 3-dimensional geometrical aspects of the scaphoid ligaments and their attachment areas by using 3-dimensional anatomical images of cadaveric wrists. The secondary aim was to verify the attachment of ligaments whose descriptions in the literature are most consistent.…”