Quantitative Detection of Chromosome Structures by Computerized Microphotometric Scanning

Drets, Máximo E.; Folle, Gustavo A.; Monteverde, Fabien

doi:10.1007/978-3-642-75682-5_1

Cited by 5 publications

(2 citation statements)

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“…The quantitative and visual information given by the interactive graphic method thus contributed to obtain more informative images of the nuclear and chromosome structure (Drets and Monteverde, 1987;Drets et al, 1989Drets et al, , 1994Drets et al, , 1995a.…”

Section: Scanning Microphotometry and Subtelomeric Structuresmentioning

confidence: 99%

Cytological indications of the complex subtelomeric structure

2004

Cytogenet Genome Res

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Research on the subtelomeric region has considerably increased because this chromosome segment (1) keeps the chromosome number constant, (2) intervenes in cancer and cell senescence processes, (3) presents more crossovers than other regions of the genome and, (4) is the site of cryptic chromosome aberrations associated with mental retardation and congenital malformations. Quantitative microphotometrical scanning and computer graphic image analysis enables the detection of differentially distributed Giemsa-stained structures in T-banded subtelomeric segments of human and Chinese hamster ovary (CHO) chromosomes. The presence of high density stain patterns in the subtelomeric region was confirmed using endoreduplicated chromosomes as a model. Besides, prolonging the incubation in the T-buffer, specific holes were induced in subtelomeric segments. Hole specificity was confirmed inducing them in complex CHO chromosome aberrations obtained by AluI. The method was also used to detect minute sister chromatid exchanges in the T-banded subtelomeric area (t-SCEs). The presence of t-SCEs was suspected to reflect, at the microscope level, the high crossover activity prevailing in the region. Due to the fact that the fluorescent signals obtained with subtelomeric probes seem to be colocalized with subtelomeric high density areas, measurements on the position of both structures with respect to the diffraction and chromosome edges were carried out. Data obtained showed comparable values suggesting that the high density segments were located where telomeric probes usually fluoresce. The possible relationship of the high density patterns, the production of specific holes, the localization of fluorescent areas and the detection of minute SCEs in the subtelomeric segment observed in T-banded CHO and human chromosomes is briefly reviewed.

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Section: Scanning Microphotometry and Subtelomeric Structuresmentioning

confidence: 99%

Cytological indications of the complex subtelomeric structure

2004

Cytogenet Genome Res

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“…We carried out an extensive research in our Laboratory along the decades trying to apply this computer graphic technology to specific cytogenetical problems developing instrumentation and dedicated software (Drets and Monteverde, 1987). The interactive system thus developed allowed the microphotometrical scanning of human G-, R-, C-and T-banded chromosomes and the quantitative localization of the highest band densitometric peaks and band-interband junctions (Drets et al, 1989). Besides, this procedure of computerized morphometric measurements enabled to draw graphic quantitative maps and to redefine chromosome banding patterns and karyotype description, all of which has proved to be useful in molecular cytogenetics (Drets et al, 1994).…”

Section: Scanning Microphotometry In Chromosome Researchmentioning

confidence: 99%