Quantitative detection of circulating epithelial cells by Q-RT-PCR

Iakovlev, Vladimir V.; Goswami, Rashmi S.; Vecchiarelli, Jonathan; Arneson, Nona; Done, Susan J.

doi:10.1007/s10549-007-9532-9

Cited by 25 publications

(13 citation statements)

References 19 publications

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“…Therefore, new procedures to isolate and enumerate CTCs are being sought out. For instance, quantitative detection of CTCs by real time quantitative RT-PCR has been being developed [13]. Other methods have also been undergoing testing: immunomagnetic isolation, fl uorescent antibody dye testing and cell enumeration by cytometry with fl uorescent microscopy [14].…”

Section: Discussionmentioning

confidence: 99%

Circulating tumour cells in locally advanced breast cancer

et al. 2009

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Section: Discussionmentioning

confidence: 99%

Circulating tumour cells in locally advanced breast cancer

et al. 2009

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“…The analysis of patient samples can be related to RQ-values of the calibration curves. Conclusions can be drawn to the number of CTCs in a patient sample [42]. …”

Section: Ctc-detection Via Real-time Pcrmentioning

confidence: 99%

Detection of Circulating Tumour Cells from Blood of Breast Cancer Patients via RT-qPCR

Andergassen

Kölbl

Hutter

et al. 2013

Cancers

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Breast cancer is still the most frequent cause of cancer-related death in women worldwide. Often death is not caused only by the primary tumour itself, but also by metastatic lesions. Today it is largely accepted, that these remote metastases arise out of cells, which detach from the primary tumour, enter circulation, settle down at secondary sites in the body and are called Circulating Tumour Cells (CTCs). The occurrence of such minimal residual diseases in the blood of breast cancer patients is mostly linked to a worse prognosis for therapy outcome and overall survival. Due to their very low frequency, the detection of CTCs is, still a technical challenge. RT-qPCR as a highly sensitive method could be an approach for CTC-detection from peripheral blood of breast cancer patients. This assumption is based on the fact that CTCs are of epithelial origin and therefore express a different gene panel than surrounding blood cells. For the technical approach it is necessary to identify appropriate marker genes and to correlate their gene expression levels to the number of tumour cells within a sample in an in vitro approach. After that, samples from adjuvant and metastatic patients can be analysed. This approach may lead to new concepts in diagnosis and treatment.

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“…The disadvantages of these methods of profiling are false expression signatures created from non-specific primers or staining, lack of expression of the marker of interest on CTCs in the sample, pseudogenes, and low expression of the marker of interest on non-malignant cells. Work done in our own laboratory demonstrated the use of RT-PCR techniques to enumerate CTCs in blood by correcting for a percentage of these types of false signatures (Iakovlev et al, 2007). Blood from healthy human volunteers was spiked with 1-10,000 tumour cells from breast cancer cell lines.…”

Section: Genetic Profiling Of Ctcsmentioning

confidence: 99%