Quantitative Detection of Residual E. coli Host Cell DNA by Real-Time PCR

Enterovirus71 virus-like particles produced from insect cells and purified by multistep chromatography elicit strong humoral immune responses in mice

“…They may potentially cause allergic reactions (proteins) or even genetic disruption (DNA) resulting in tumours (Lee et al . ). Therefore, a large number of reports on process development have chosen HCP and host cell DNA clearance as a benchmark to demonstrate a well‐controlled bioprocess.…”

Magnetic Nanoparticles PCR Enzyme‐Linked Gene Assay for Quantitative Detection of BCR/ABL Fusion Gene in Chronic Myelogenous Leukemia

“…Similar to the previous work, the primers used in the MELGA technique displayed high specificity for the leukemic K562 cell line ( BCR/ABL expression). The detection limit for this technique is <1 fg/ml of RNA in the K562 cell line, which is lower than RQ‐PCR , conventional PCR , and ELISA (i.e., picogram, nanogram, and nanogram, respectively). At <1 fg/ml of total RNA, the OD was slightly increased.…”

Design and validation of a new method to detect and quantify residual host cell DNA in human recombinant erythropoietin (rEPO)

“…Other parameters such as cost, experienced personnel, interpretability, validity of results, and applicability are among the factors that influence the approval of final results. Therefore, the functional characteristics of each method used should be evaluated [30][31][32][33]. Even though easy to set up conventional radiolabeled hybridization based methods have low precision due to the instability and short life-span of radio-isotopes [19].…”