Quantitative detection of well-based DNA array using switchable lanthanide luminescence

Lanthanide light for biology and medical diagnosis

“…Nucleic acid and protein microarrays are becoming popular techniques in luminescent immunoassays for proteomics and diagnostics [76]. The full range of LLB-based analyses is of course available for these technologies and relevant protocols start to be proposed [68,77] both for well-type analysis featuring solid-state arrays [78] and for sophisticated microarrays [68,77].…”

Switchable lanthanide luminescent binary probes in efficient single nucleotide mismatch discrimination

“…We have previously used switchable lanthanide luminescence for homogeneous nucleic acid [17,29,30] and protein [16,31] detection in solution and in homogeneous DNA-based array format [18]. Luminescent complex has previously been reported to form from adjacent hybridization of binary probes on a target nucleic acid in solution [32][33][34].…”

“…1) were labelled with isothiocyanate-activated form of the ion carrier chelate DOTA-Eu III [2,2 ,2 -(10-(3-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)tri(acetate)europium(III)] [16] and the two 5 -aminomodified probes (A2 and B2 in Fig. 1) were labelled with isothiocyanate-activated form of the light absorbing antenna ligand [4-((isothiocyanatophenyl)ethynyl)pyridine-2,6-dicarboxylicacid] [17] as described previously [18].…”

Platforms for Bioorthogonal Oligonucleotide-templated Reactions: Translating Concepts into Devices

“…In the absence of a complementary target oligonucleotide, the detection probe remained in the liquid phase and was therefore undetectable at the spot. This homogeneous solid-phase array-based method resulted in quantitative detection of synthetic target oligonucleotides with a sensitivity in the nM range [50]. When compared to traditional organic fluorophores, luminescent lanthanide ions or complexes offer the advantage of a longer emission lifetime, sharper emission peaks and typically larger Stokes' shift [51].…”