Quantitative determination of 5-hydroxyindole-3-acetic acid in cerebrospinal fluid by gas chromatography-mass spectrometry

Bertilsson, Leif; Atkinson, Arthur J.; Althaus, James R.; Härfast, Åse; Lindgren, Jan‐Erik; Holmstedt, Bo

doi:10.1021/ac60316a056

Cited by 111 publications

(17 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CSF samples were centrifuged and immediately frozen in 3 ml aliquots in silanized glass tubes and stored at -2OOC until analysis. The amine metabolites 5-hydroxyindoleacetic acid (5-HIAA), homovanillic acid (HVA) and 4-hydroxy-3-methoxy-phenylglycol (HMPG) were analyzed in CSF by mass fragmentographic methods (Bertilsson et al (1972, Bertilsson (1973)) as recently modified (Bertilsson (1980)). The three samples from each patient were analyzed at the same time.…”

Section: Methodsmentioning

confidence: 99%

Bromocriptine treatment of depressive disorders

Nordin

Siwers

Bertilsson

1981

Acta Psychiatr Scand

View full text Add to dashboard Cite

Fifteen depressed patients were treated with increasing doses of bromocriptine in an open study. Twelve were treated for 5 weeks (final dose 20-60 mg daily) and nine of these recovered almost completely. As expected from a dopamine agonist, bromocriptine decreased the level of homovanillic acid (HVA) in cerebrospinal fluid (CSF) by 15% (P less than 0.05) and 23% (P less than 0.01) after 2 and 5 weeks of treatment, respectively. After 2 but not after 5 weeks of treatment there was also a small but significant decrease (13%; P less than 0.001) of the noradrenaline metabolite HMPG in CSF. Although there was no mean effect on 5-HIAA in CSF, there was a significant relationship between the HVA and 5-HIAA levels (as % of pretreatment level) both after 2 and 5 weeks of treatment (r = 0.96 and r = 0.62, respectively). This may indicate that the drug has an effect on the serotonin system secondary to the dopamine receptor stimulation. The amelioration of depression was not related to HVA, but did correlate to HMPG in CSF (r = 0.65; P less than 0.05) both metabolites measured before treatment. These results indicate that bromocriptine may have antidepressant effects possibly mediated through the noradrenergic system rather than the dopaminergic system.

show abstract

Section: Methodsmentioning

confidence: 99%

Bromocriptine treatment of depressive disorders

Nordin

Siwers

Bertilsson

1981

Acta Psychiatr Scand

View full text Add to dashboard Cite

show abstract

“…A very sensitive and specific assay for 5-hydroxyindole-3-acetic acid in human cerebrospinal fluid was reported based on GC-mass spectrometry analysis of the diheptafluorobutyryl methyl ester derivative (202). The two major ions (mle 538 and 597) of the mass spectrum were monitored after elution from the chromatographic column.…”

Section: Methods-stablementioning

confidence: 99%

Studies with Deuterated Drugs

Blake

Crespi

Katz

1975

Journal of Pharmaceutical Sciences

116

View full text Add to dashboard Cite

“…Two-ml aliquots were analyzed in duplicate for their content of 5-HIAA and IAA according to Bertilsson and associates 5 and Bertilsson and Palmer.6 For each patient, both samples were analyzed at the same time.…”

Section: Methodsmentioning

confidence: 99%