Quantitative Determination of Acetic Acid in Gefitinib by Reverse Phase HPLC

Rawat, Ajay Singh; Chauhan, Kamlesh R.; Parmar, Y. S.; Sannigrahi, Pooja; Patel, Divyesh; Belwal, Chandrakant; Vardhan, Anand

doi:10.7598/cst2014.681

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…The determination of SCFA followed the procedure from Rawat [26]. Preparation of ALRP standard solution by performing multilevel dilutions with concentrations of 0,001, 0,005, 0,010, 0,025, and 0,050 mg/mL from 0,100 mg/mL common solution.…”

Section: Determination Of Short Chain Fatty Acidmentioning

confidence: 99%

Potency of fermented jicama extract cultured with Lactobacillus plantarum B1765 for producing short chain fatty acid

Wijayanti,

Wikandari

2023

J. Pijar.MIPA

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Short-chain fatty acid (SCFA) is the end product of inulin metabolism, which has various health benefits, such as affecting lipid metabolism and as an antidiabetic agent. This research studied the effect of the fermentation time of jicama extract with Lactobacillus plantarumB1765 as a starter culture on producing SCFA. Fermentation was conducted for 2, 12, 24, and 36 hours at a temperature of 37°C with 5% (v/v) of the starter culture, then determined the growth of LAB, pH, Total Titratable Acid (TTA), fructose, and Short Chain Fatty Acid content. The total LAB count was measured using the Total Plate Count; fructose was measured using the Nelson-Somogyi method, while SCFA was measured using HPLC with eluents H3PO4 0.1% and acetonitrile 25%. The length of fermentation affected the increase in total Lactic Acid Bacteria (LAB), Total titratable acidity (TTA), fructose, SCFA, and the decrease in pH. The results showed that the optimal growth of Total LAB at 24 hours of fermentation correlated with fructose content, but increasing TTA and SCFA until 36 hours of fermentation and pH decreased to 3,92 at the end of fermentation and decreasing pH due to lactic acid and SCFA producing. SCFA was identified as consisting of acetic acid (1.31±0.006 mg/mL), propionic acid (1.58±0.0055mg/mL), and butyric acid (1.05±0.0038mg/mL), with the highest SCFA was propionic acid. Therefore, fermented jicama extract with Lactobacillus plantarum B1765 has the potential to produce SCFA, which could be used for various health benefits.

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Section: Determination Of Short Chain Fatty Acidmentioning

confidence: 99%

Potency of fermented jicama extract cultured with Lactobacillus plantarum B1765 for producing short chain fatty acid

Wijayanti,

Wikandari

2023

J. Pijar.MIPA

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“…The other symptoms are headaches, eye pain, and nausea. [5][6][7][8] It was determined in vinegar, 9 wine, 10,11 fermentation broth, 12 gefitinib, 13 birch wood 14 and heritage collections. 15 During the synthesis of pharmaceuticals were required residual solvent in the process.…”

Section: Introductionmentioning

confidence: 99%

Optimized and Validated Box–Behnken Design Combined Response Surface Methodology to Quantify Acetic Acid Content in Potassium Clavulanate Using High–Performance Liquid Chromatography

Haque

2022

Yanbu Journal of Engineering and Science

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A simple and robust high-performance liquid chromatography method with UV detection was developed to quantify acetic acid in potassium clavulanate. The chromatographic separation was performed on the Hypersil BDS C18 column (250×4.6 mm, 5 μm) with optimized response surface methodology combined Box–Behnken experimental design. The mobile phase comprises of a buffer prepared by ammonium dihydrogen orthophosphate (20 mM) containing 0.01% triethylamine, maintained pH between 2.8–3.2 using phosphoric acid and % acetonitrile as per Box–Behnken factorial design. The detection was performed at 235 nm with a mobile phase flow rate of 1 ml/min. The method was validated as per the ICH guidelines. The linearity of the proposed method was rectilinear within the range of 2.5–24.8 μg/ml. The value of the limit of detection (LOD) & limit of quantitation (LOQ) by the validated method was found to be 1.9 and 5.8 μg/ml, respectively. The robustness and ruggedness studies were conducted by small changes with parameters in the proposed method. The % RSD values were within the accepted limit of criteria. This developed method can quantify routine acetic acid content analysis in potassium clavulanate pharmaceutical formulations.

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A New stability indicating RP-HPLC method for the estimation of Gefitinib tablets using an ion pairing agent

Aluri

Annapurna

2021

RJPT

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Gefitinib is an anticancer drug used for the treatment of lung cancer, breast cancer and prostate cancer. A new stability indicating RP-HPLC method was proposed for the estimation of Gefitinib in pharmaceutical dosage forms (tablets). Shimadzu Model CBM-20A/20 Alite HPLC system with PDA detector and Agilent C18 column were used for the chromatographic study. Mobile phase mixture consisting of Tetra butyl ammonium hydrogen sulphate and Methanol in the ratio 50:50, v/v with a flow rate 0.8 mL/min was chosen for the chromatographic elution of Gefitinib (Detection wavelength 340 nm). The method was linear over the concentration range 0.1-80 mg/mL with linear regression equation, y = 70782x + 6171.6 (R² = 0.9999). The LOD and LOQ were found to be 0.2931 mg/mL and 0.8947 mg/mL respectively. Stress degradation studies were performed by exposing Gefitinib to various stress conditions and the method was validated as per ICH guidelines.

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Quantitative Determination of Acetic Acid in Gefitinib by Reverse Phase HPLC

Cited by 3 publications

References 4 publications

Potency of fermented jicama extract cultured with Lactobacillus plantarum B1765 for producing short chain fatty acid

Potency of fermented jicama extract cultured with Lactobacillus plantarum B1765 for producing short chain fatty acid

Optimized and Validated Box–Behnken Design Combined Response Surface Methodology to Quantify Acetic Acid Content in Potassium Clavulanate Using High–Performance Liquid Chromatography

A New stability indicating RP-HPLC method for the estimation of Gefitinib tablets using an ion pairing agent

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