Quantitative determination of dehydroepiandrosterone fatty acyl esters in human female adipose tissue and serum using mass spectrometric methods

Wang, Feng; Koskela, Anja; Hämäläinen, Esa; Turpeinen, Ursula; Savolainen-Peltonen, Hanna; Mikkola, Tomi S.; Vihma, Veera; Adlercreutz, Herman; Tikkanen, Matti J.

doi:10.1016/j.jsbmb.2011.01.014

Cited by 16 publications

(10 citation statements)

References 36 publications

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“…Despite experimental studies showing fatty acyl esterification of DHEA (142), no endogenous DHEA-FAE was detected in human female visceral adipose tissue (147). This might be related to the active hydrolysis of DHEA-FAE by HSL in adipose tissue (142).…”

Section: Steroid Fatty Acyl Esters As the Storage Form Of Active Stermentioning

confidence: 82%

Steroid biosynthesis in adipose tissue

2015

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Section: Steroid Fatty Acyl Esters As the Storage Form Of Active Stermentioning

confidence: 82%

Steroid biosynthesis in adipose tissue

2015

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“…Blood samples were obtained in the morning between 0700 and 0900 h after an overnight fast and stored at −80C until assay. Concentrations of serum dehydroepiandrosterone (DHEA) [23], testosterone [24], DHT [25], E2 [26], and E1 [27] were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) as described previously. Serum SHBG, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) were determined by chemiluminescent enzyme immunoassay (Siemens Healthcare Diagnostics) using an Immulite 2000 Xpi analyzer.…”

Section: Quantification Of Serum Hormones and Other Serum Analysesmentioning

confidence: 99%

Metabolism of sex steroids is influenced by acquired adiposity—A study of young adult male monozygotic twin pairs

Vihma

Naukkarinen

Turpeinen

et al. 2017

The Journal of Steroid Biochemistry and Molecular Biology

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Obesity and ageing are associated with lower serum testosterone levels in men. How fat distribution or adipose tissue metabolism, independent of genetic factors and age, are related to sex steroid metabolism is less clear. We studied the associations between adiposity and serum sex hormone concentrations, and mRNA expression of genes regulating sex hormone metabolism in adipose tissue in young adult male monozygotic (MZ) twin pairs. The subjects [n=18 pairs; mean age, 32 years; individual body mass indexes (BMIs) 22-36kg/m] included 9 male MZ twin pairs discordant for BMI [intra-pair difference (Δ) in BMI ≥3kg/m]. Sex steroid concentrations were determined by liquid chromatography-tandem mass spectrometry, body composition by dual-energy X-ray absorptiometry and magnetic resonance imaging, and mRNA expressions from subcutaneous adipose tissue by Affymetrix. In BMI-discordant pairs (mean ΔBMI=5.9kg/m), serum dihydrotestosterone (DHT) was lower [mean 1.9 (SD 0.7) vs. 2.4 (1.0) nmol/l, P=0.040] and mRNA expressions of DHT-inactivating AKR1C2 (P=0.021) and cortisol-producing HSD11B1 (P=0.008) higher in the heavier compared to the leaner co-twins. Serum free 17β-estradiol (E2) was higher [2.3 (0.5) vs. 1.9 (0.5) pmol/l, P=0.028], and in all twin pairs, serum E2 and estrone concentrations were higher in the heavier than in the leaner co-twins [107 (28) vs. 90 (22) pmol/l, P=0.006; and 123 (43) vs. 105 (27) pmol/l, P=0.025]. Within all twin pairs, i.e. independent of genetic effects and age, 1) the amount of subcutaneous fat inversely correlated with serum total and free testosterone, DHT, and sex hormone-binding globulin (SHBG) concentrations (P<0.01 for all), 2) intra-abdominal fat with total testosterone and SHBG (P<0.05), and 3) liver fat with SHBG (P=0.006). Also, 4) general and intra-abdominal adiposity correlated positively with mRNA expressions of AKR1C2, HSD11B1, and aromatase in adipose tissue (P<0.05). In conclusion, acquired adiposity was associated with decreased serum DHT and increased estrogen concentrations, independent of genetic factors and age. The reduction of DHT could be linked to its increased degradation (by AKR1C2 and HSD11B1) and increased estrogen levels to increased adiposity-related expression of aromatase in adipose tissue.

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“…Initial steps of de novo steroid biosynthesis include mobilisation of cholesterol into inner mitochondrial membrane by steroidogenic acute regulatory protein (StAR), and subsequent cholesterol sidechain cleavage by the mitochondrial enzyme, cholesterol side-chain cleavage enzyme, P450scc (CYP11A1) (Savolainen-Peltonen et al, 2014;Wang et al, 2011). Initial steps of de novo steroid biosynthesis include mobilisation of cholesterol into inner mitochondrial membrane by steroidogenic acute regulatory protein (StAR), and subsequent cholesterol sidechain cleavage by the mitochondrial enzyme, cholesterol side-chain cleavage enzyme, P450scc (CYP11A1) (Savolainen-Peltonen et al, 2014;Wang et al, 2011).…”

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confidence: 99%

“…placenta perform de novo steroid hormone synthesis from cholesterol. Initial steps of de novo steroid biosynthesis include mobilisation of cholesterol into inner mitochondrial membrane by steroidogenic acute regulatory protein (StAR), and subsequent cholesterol sidechain cleavage by the mitochondrial enzyme, cholesterol side-chain cleavage enzyme, P450scc (CYP11A1) (Savolainen-Peltonen et al, 2014;Wang et al, 2011). Recent discoveries on the presence of the mitochondrial cholesterol transport and metabolism machinery, including StAR and CYP11A1, suggest that AT have the ability to metabolize steroids (MacKenzie et al, 2008).…”

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confidence: 99%

Dietary trans and saturated fatty acids effects on semen quality, hormonal levels and expression of genes related to steroid metabolism in mouse adipose tissue

et al. 2019

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| INTRODUC TI ONAdipose tissue (AT) is not only an energy storage but also an active endocrine and metabolic organ contributing to the metabolic homeostasis by secreting and metabolizing a large variety of adipokines and hormones (Kershaw & Flier, 2004). It has been suggested that accumulation of visceral AT be associated with metabolic alterations that can increase the risk of metabolic syndrome, type 2 diabetes and cardiovascular diseases, whereas the crucial roles of AT in infertility were not completely understood (Fox et al., 2007). Indeed, oestrogens and other sex steroids influence metabolic function at the AT in different parts of the body, by regulating lipolysis and AT deposition. Hence, achievement of a better understanding of the regulatory roles of enzymes/genes in AT, via either endocrine, paracrine/autocrine or intracrine systems, have gained a priority in research (Li, Papadopoulos, & Vihma, 2015).Although AT is undoubtedly a major reservoir for steroid hormones in male, limited information exists on the roles of dietary fatty acids on the expression levels of genes involved in male steroidogenesis. Classical steroidogenic tissues, such as gonads, adrenals and AbstractOur objectives were to assess sperm alteration and adipose tissue (AT) genes expression related to steroid metabolism subsequent to fatty acids consumption. Twentynine mature male mice were divided into: fat diet (FD; n = 15) and the control group (n = 14). FD group was fed with low level of trans and saturated fatty acids source for 60 days. Sperm parameters, levels of hormones and the mRNA abundance of the target genes in AT were assessed. The sperm concentration, total and progressive motilities were lower in FD group compared to that of control (p < 0.01). Blood estradiol levels increased in FD (p < 0.001), whereas no significant difference was observed in testosterone. The mRNA levels of StAR, CYP11A1, CYP17A1, 17βHSD7 and 17βHSD12 in AT of FD were higher than those of the control (p < 0.05). In contrast, mRNA level of Cyp19a1 in FD was significantly (p < 0.05) lower than that of control.17βHSD12 and 17βHSD7 (as oestrogenic genes) increased, while 17βHSD5 and 17βHSD3 (as androgenic genes) remained unchanged, indicating that dietary trans/ saturated fatty acids affect AT genes expression. Probably, sperm parameters were altered by increment of expression level of genes involved in oestrogenic metabolism rather than those engaged in androgenic metabolism after fatty acids consumption. K E Y W O R D Sadipose tissue, dietary fatty acids, semen quality, steroidogenic enzyme gene expression

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Quantitative determination of dehydroepiandrosterone fatty acyl esters in human female adipose tissue and serum using mass spectrometric methods

Cited by 16 publications

References 36 publications

Steroid biosynthesis in adipose tissue

Steroid biosynthesis in adipose tissue

Metabolism of sex steroids is influenced by acquired adiposity—A study of young adult male monozygotic twin pairs

Dietary trans and saturated fatty acids effects on semen quality, hormonal levels and expression of genes related to steroid metabolism in mouse adipose tissue

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