Quantitative Determination of Dissolved Oxygen: Ascorbic Acid Oxidase Method

Sharp, Paul F.; Hand, David B.; Guthrie, E.S.

doi:10.1021/i560097a003

Cited by 20 publications

(5 citation statements)

References 10 publications

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“…(6 inches) in length, is satisfactory for this purpose. The cucumbers should be frozen (the authors have found it convenient to store the cucumbers in the frozen state for periods as long as one year) and thawed, after which the juice may be drained and concentrated to Vnth the original volume by pervaporation (17) and filtered. Such preparations have been kept for several weeks when stored at 10°C.…”

mentioning

confidence: 99%

Determination of Vitamin C in Presence of Interfering Reducing Substances Selective Oxidation-Reduction Method

Stewart¹,

Sharp²

1945

Ind. Eng. Chem. Anal. Ed.

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mentioning

confidence: 99%

Determination of Vitamin C in Presence of Interfering Reducing Substances Selective Oxidation-Reduction Method

Stewart¹,

Sharp²

1945

Ind. Eng. Chem. Anal. Ed.

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“…Small molecules that contribute to redox homeostasis include vitamin C, vitamin E, and N-acetyl cysteine (NAC). Vitamin C is a water-soluble antioxidant that protects against cholesterol oxidation, scavenges dissolved O 2 , and neutralizes radical ROS, while vitamin E is a lipid soluble radical scavenger that can be regenerated by vitamin C [ 68 , 70 , 71 , 72 ]. NAC is a glutathione precursor with a redox active thiol that neutralizes ROS and can reverse deleterious disulfide formations [ 73 ].…”

Section: Reactive Oxygen Species—neutralization Necessity and Pathologymentioning

confidence: 99%

p66Shc in Cardiovascular Pathology

Haslem

Hays

2022

Cells

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p66Shc is a widely expressed protein that governs a variety of cardiovascular pathologies by generating, and exacerbating, pro-apoptotic ROS signals. Here, we review p66Shc’s connections to reactive oxygen species, expression, localization, and discuss p66Shc signaling and mitochondrial functions. Emphasis is placed on recent p66Shc mitochondrial function discoveries including structure/function relationships, ROS identity and regulation, mechanistic insights, and how p66Shc-cyt c interactions can influence p66Shc mitochondrial function. Based on recent findings, a new p66Shc mitochondrial function model is also put forth wherein p66Shc acts as a rheostat that can promote or antagonize apoptosis. A discussion of how the revised p66Shc model fits previous findings in p66Shc-mediated cardiovascular pathology follows.

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“…The classical titrimetric and colorimetric methods for oxygen determination, such as the Winkler method, are not applicable to orange juice because of the organic materials present. The ascorbic acid oxidase method used by Sharp et al (19) to determine oxygen in milk is also inapplicable, since the enzyme is inactive at the pH of orange juice. There remain methods based on removal of the dissolved gases from a sample under reduced pressure, measurement of the volume or pressure of the mixed gases, and analysis of the mixture.…”

Section: Calibrationmentioning

confidence: 99%

“…The sample is collected and introduced into the polarographic cell using techniques designed to avoid air contamination, similar to those used by Sharp et al (19) when sampling milk for oxygen determination. The current flowfing at an applied potential of -0.4 volt is measured at an appropriate galvanometer sensitivity, then the temperature of the juice is recorded and its soluble solids content determined by means of a Brix hydrometer or a refractometer.…”

Section: Recommended Proceduresmentioning

confidence: 99%