Quantitative determination of dopamine in human plasma by a highly sensitive LC–MS/MS assay: Application in preterm neonates

Zhang, Daping; Wu, Lei; Chow, Diana; Tam, Vincent H.; Rios, Danielle R.

doi:10.1016/j.jpba.2015.09.003

Cited by 33 publications

(23 citation statements)

References 15 publications

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“…Dopamine isotopic enrichment was determined as native dopamine and as the n-propyl derivative [27] (diester, amide) by LC-MS/MS (Shimazu UFLCXR, QTRAP 6500) by negative electrospray ionization. Three parent-daughter ion pairs were monitored to confirm peak identification as dopamine (154/137, 154/119, and 154/91), or as its n-propyl derivative (322/137, 322/210, and 322/266).…”

Section: Methodsmentioning

confidence: 99%

A role for foregut tyrosine metabolism in glucose tolerance

Körner¹,

Cline²,

Slifstein³

et al. 2019

Molecular Metabolism

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Objective We hypothesized that DA and L-DOPA derived from nutritional tyrosine and the resultant observed postprandial plasma excursions of L-DOPA and DA might affect glucose tolerance via their ability to be taken-up by beta cells and inhibit glucose-stimulated β-cell insulin secretion. Methods To investigate a possible circuit between meal-stimulated 3,4-dihydroxy-L-phenylalanine (L-DOPA) and dopamine (DA) production in the GI tract and pancreatic β-cells, we: 1) mapped GI mucosal expression of tyrosine hydroxylase (TH) and aromatic amino acid decarboxylase (AADC); 2) measured L-DOPA and DA content of GI mucosal tissues following meal challenges with different L-tyrosine (TYR) content, 3) determined whether meal TYR content impacts plasma insulin and glucose excursions; and 4) characterized postprandial plasma excursions of L-DOPA and DA in response to meal tyrosine content in rodents and a population of bariatric surgery patients. Next, we characterized: 1) the metabolic transformation of TYR and L-DOPA into DA in vitro using purified islet tissue; 2) the metabolic transformation of orally administrated stable isotope labeled TYR into pancreatic DA, and 3) using a nuclear medicine technique, we studied endocrine beta cells in situ release and binding of DA in response to a glucose challenge. Results We demonstrate in rodents that intestinal content and circulatory concentrations L-DOPA and DA, plasma glucose and insulin are responsive to the tyrosine (TYR) content of a test meal. Intestinal expression of two enzymes, Tyrosine hydroxylase (TH) and Aromatic Amino acid Decarboxylase (AADC), essential to the transformation of TYR to DA was mapped and the metabolism of metabolism of TYR to DA was traced in human islets and a rodent beta cell line in vitro and from gut to the pancreas in vivo. Lastly, we show that β cells secrete and bind DA in situ in response to glucose stimulation. Conclusions We provide proof-of-principle evidence for the existence of a novel postprandial circuit of glucose homeostasis dependent on nutritional tyrosine. DA and L-DOPA derived from nutritional tyrosine may serve to defend against hypoglycemia via inhibition of glucose-stimulated β-cell insulin secretion as proposed by the anti-incretin hypothesis.

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Section: Methodsmentioning

confidence: 99%

A role for foregut tyrosine metabolism in glucose tolerance

Körner¹,

Cline²,

Slifstein³

et al. 2019

Molecular Metabolism

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“…During SPE, steps including the removal of interfering compounds and the enrichment of the analytes with different types of sorbents, such as: strong cation exchange (SCX), weak cation exchange (WCX) and mixed mode cation exchange (MXC), hydrophilic-lipophilic balanced copolymer (HLB), alumina B and C18, during the isolation of BAs from human matrices, were investigated. Moreover, SPE can be used to isolate analytes of interest from a wide variety of matrices, including urine, whole blood, plasma, serum, cerebrospinal fluid or human peripheral blood mononuclear cells [16,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67].…”

Section: Sample Preparationmentioning

confidence: 99%

“…In order for a compound to be retained by ion exchange from an aqueous solution, the pH of the sample matrix must be one at which both the compound of interest and the functional group on the bonded silica are charged. As was reported, silica-bonded sulfonic acid with Na + counterion (SCX) for strong cation exchange was rarely used by Zhang et al [54]. For the isolation of catecholamines from plasma or urine, WCX cartridges with silica-bonded carboxylic acid with Na + counterion were more popular [53,55,64,68].…”

Section: Sample Preparationmentioning

confidence: 99%

“…Another type of ionization, such as atmospheric pressure chemical ionization (APCI), which is preferred for molecules with a low to medium polarity, was also applied for the quantification of BAs in urine samples [46]. The chromatograms were mainly extracted in MRM [25,26,29,30,31,35,41,42,44,54,55,59,60,62,64,65,68,76,77]; although the SRM mode was also used for BA quantifications [7,28,57]. However, analysis in MRM mode is preferred in clinical practice to avoid false-positive results.…”

Section: Analytical Strategies Of Ba Determinations Based On Separmentioning

confidence: 99%

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Recent Trends in the Quantification of Biogenic Amines in Biofluids as Biomarkers of Various Disorders: A Review

Plenis

Olędzka

Kowalski

et al. 2019

JCM

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Biogenic amines (BAs) are bioactive endogenous compounds which play a significant physiological role in many cell processes like cell proliferation and differentiation, signal transduction and membrane stability. Likewise, they are important in the regulation of body temperature, the increase/decrease of blood pressure or intake of nutrition, as well as in the synthesis of nucleic acids and proteins, hormones and alkaloids. Additionally, it was confirmed that these compounds can be considered as useful biomarkers for the diagnosis, therapy and prognosis of several neuroendocrine and cardiovascular disorders, including neuroendocrine tumours (NET), schizophrenia and Parkinson’s Disease. Due to the fact that BAs are chemically unstable, light-sensitive and possess a high tendency for spontaneous oxidation and decomposition at high pH values, their determination is a real challenge. Moreover, their concentrations in biological matrices are extremely low. These issues make the measurement of BA levels in biological matrices problematic and the application of reliable bioanalytical methods for the extraction and determination of these molecules is needed. This article presents an overview of the most recent trends in the quantification of BAs in human samples with a special focus on liquid chromatography (LC), gas chromatography (GC) and capillary electrophoresis (CE) techniques. Thus, new approaches and technical possibilities applied in these methodologies for the assessment of BA profiles in human samples and the priorities for future research are reported and critically discussed. Moreover, the most important applications of LC, GC and CE in pharmacology, psychology, oncology and clinical endocrinology in the area of the analysis of BAs for the diagnosis, follow-up and monitoring of the therapy of various health disorders are presented and critically evaluated.

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“…Due to the difficulty of detecting native neurochemicals, several in‐situ derivatization that enhance LC retention and MS sensitivity has been explored [18–20]. Up to now, certain solvents have been used as derivatizing reagents including propionic anhydride [21], ethyl chloroformate [22], formaldehyde [23], dansyl chloride (DNSCl) [24,25], and benzoyl chloride (BzCl) [26,27,28]. Particularly, this reference [28] introduced the detection of 70 neurologically relevant compounds (not including MLT) in biological samples using BzCl derivatization.…”

Section: Introductionmentioning

confidence: 99%

Broad targeted analysis of neurochemicals in rat serum using liquid chromatography tandem mass spectrometry with chemical derivatization

Meng

Bai

et al. 2020

J of Separation Science

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In this study, an efficient and sensitive assay for the detection of 42 polar neurochemicals, including neurotransmitters, amino acids, and biogenic amines, was established by combining reversed‐phase liquid chromatography tandem mass spectrometry with chemical derivatization. An optimally designed benzoyl chloride derivatization was easily conducted in a one‐pot reaction and stable neurochemical derivatives were obtained under mild conditions within 5 min (except for acetylcholine and melatonin). Derivatization also enabled the introduction of heavy labeling of the analytes through the use of labeled derivatization agents. Chromatography separation was performed on an HSS T3 column within 15 min by gradient elution. Multiple reaction monitoring acquisition mode enabled quantitation of neurochemicals with limits of detection of 0.05 to 11.63 nM and lower limits of quantitation of 0.09 to 46.50 nM in rat serum. The assay was well validated in terms of linearity and extraction recovery. Furthermore, the instrumental precision, specificity, matrix effect, accuracy, precision, stability, dilution effect, and carry‐over effect were also validated. Finally, the overall efficacy of the assay was experimentally tested using serum from six Sprague–Dawley rats. The results demonstrated that the developed method is effective for broad targeted analysis of 42 neurochemicals in serum.

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Quantitative determination of dopamine in human plasma by a highly sensitive LC–MS/MS assay: Application in preterm neonates

Cited by 33 publications

References 15 publications

A role for foregut tyrosine metabolism in glucose tolerance

A role for foregut tyrosine metabolism in glucose tolerance

Recent Trends in the Quantification of Biogenic Amines in Biofluids as Biomarkers of Various Disorders: A Review

Broad targeted analysis of neurochemicals in rat serum using liquid chromatography tandem mass spectrometry with chemical derivatization

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