Quantitative determination of endotoxins on surgical gloves

Peiró, S.Asplund; Kulander, Lena; Eriksson, Ola

doi:10.1016/0195-6701(90)90061-r

Cited by 14 publications

(7 citation statements)

References 7 publications

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“…36,37 A few studies have demonstrated LPS contamination on some surgical latex gloves using the LAL assay. Peiro et al 6 reported that heavy LPS contamination was observed on the outside of certain surgical gloves sold in Sweden. Holmdahl and Chegini 5 reported that glove powder can act as a vehicle for latex allergens, LPS, and possibly bacteria, because powder particles can become airborne and are easily spread.…”

Section: Discussionmentioning

confidence: 99%

Chemical and biological evaluation of endotoxin contamination on natural rubber latex products

Haishima

Murai

Nakagawa

et al. 2001

J. Biomed. Mater. Res.

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Relationship between pyrogenicity and bacterial endotoxin contamination on latex products was demonstrated by chemical analysis and biological assays. In commercially available latex products' surveillance, water extracts prepared from one surgical glove and two silicone elastomer-coated Foley catheters sterilized by gamma-irradiation were obviously pyrogenic in rabbits. The induced fever was monophasic at low dose of the pyrogenic extracts and biphasic at high dose. These extracts exhibited limulus amebocyte lysate gelation activity, and induced inflammatory cytokine (interleukin-1, interleukin-6, and tumor necrosis factor-alpha) production from MM6-CA8 human monocytoid cells. These biological properties, including pyrogenicity, completely disappeared by treating the pyrogenic extracts with endotoxin-adsorbent affinity column. Limulus amebocyte lysate activity and cytokine production from MM6-CA8 cells induced by the extracts were significantly decreased by endotoxin inhibitors, an active fragment peptide of an 18-kDa cationic antimicrobial protein and a synthetic lipid A B464 analogue. Furthermore, very small amounts of 2-keto-3-deoxyoctonate and 3-hydroxy fatty acid, which are common constituents of bacterial endotoxins, were detected by gas chromatography-mass spectrometry analysis of the pyrogenic extracts. These findings clearly showed that the pyrogenicity found in these latex products originated from endotoxins contaminating the products.

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Section: Discussionmentioning

confidence: 99%

Chemical and biological evaluation of endotoxin contamination on natural rubber latex products

Haishima

Murai

Nakagawa

et al. 2001

J. Biomed. Mater. Res.

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“…Badania poziomu kontaminacji endotoksynami jałowych rękawic chirurgicznych przeprowadzili także Asplund Peiro i wsp. [17], którzy dla rękawic pochodzących z 16 różnych partii stwierdzili poziom w zakresie od 0,2 do 1071 EU/rękawicę. Autorzy ci zaproponowali trzy stopnie "czystości" w odniesieniu do zawartości endotoksyn: niewielki poziom dla zakresu od 0,2 do 9,0 EU/rękawicę, średni dla zakresu od 15 do 31 EU/rękawicę oraz wysoki dla zakresu 138 do 1071 EU/rękawicę.…”

Section: Introductionunclassified

Endotoxins and pyrogens in the context of the use of protective gloves in hospital environment

Różańska¹

2019

medicine

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STRESZCZENIE:Bezpieczeństwo pacjentów narażonych na czynniki biologiczne w trakcie procedur diagnostycznych i leczniczych wymaga przestrzegania zasad izolacji, właściwych dla konkretnego pacjenta i wykonywanych czynności. Podczas wykonywania procedur związanych z ekspozycją na krew i płyny ustrojowe konieczne jest stosowanie rękawiczek ochronnych, w większości sytuacji niejałowych, jednorazowego użycia, natomiast w trakcie zabiegów operacyjnych -rękawiczek jałowych. Rękawiczki dopuszczone do użycia muszą spełniać określone wymagania w zakresie wytrzymałości na działanie czynników fizycznych i chemicznych oraz ochrony przed czynnikami biologicznymi -drobnoustrojami jak również endotoksynami. Endotoksyna, czyli lipopolisacharyd (LPS) będący integralnym składnikiem zewnętrznej błony komórkowej bakterii Gram-ujemnych, jest zaliczana do typowych czynników pirogennych. Jej obecność w organizmie człowieka w określonych sytuacjach może prowadzić do wystąpienia różnego rodzaju niekorzystnych efektów, w tym nawet wstrząsu septycznego. Powszechne występowanie endotoksyny w środowisku człowieka oraz jej duża odporność na czynniki fizyczne stawia przed producentami wyrobów medycznych, w tym rękawiczek jałowych, szczególne wymagania dotyczące zachowania czystości środowiska oraz jej kontroli na każdym etapie produkcji.SŁOWA KLUCZOWE: endotoksyna, LPS, rękawiczki ochronne, test LAL, bezpieczeństwo pacjentów ABSTRACT:Ensuring patient safety in relation to exposure to biological agents during diagnostic and therapeutic procedures requires compliance with the rules of isolation specific to a particular patient and performed activities. Procedures related to exposure to blood and body fluids require the use of protective gloves -single use, non-sterile ones in most situations, and sterile gloves during surgical operations. Gloves approved for use must meet specific requirements for resistance to physical and chemical agents and protection against biological agents m

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“…5 Therefore, the endotoxin in parenteral pharmaceuticals is strictly controlled. With regard to medical devices, it has been reported that contaminating endotoxin exhibits pyrogenicity, 6 induces the production of proinflammatory cytokines, 7 and furthermore acts as an allergen 8,9 synergistically with the source of the irritation to induce allergy and inflammation. 10 Therefore, special attention should also be paid to the endotoxin contamination of medical devices.…”

Section: Introductionmentioning

confidence: 99%

Endotoxin contamination in wound dressings made of natural biomaterials

et al. 2003

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Contamination by endotoxin of nine kinds of wound dressings made of natural biomaterials (calcium alginate, collagen, chitin, and poly-L-leucine) was examined with the use of water extracts. By applying the Limulus amoebocyte lysate (LAL) test, high concentrations of endotoxin were detected in extracts from three kinds of products made of calcium alginate. These extracts evoked fever in rabbits and induced the release of a proinflammatory (pyrogenic) cytokine, interleukin-6 (IL-6), from human monocytic cells (MM6-CA8). The effects disappeared when the extracts were treated with endotoxin-removing gel column chromatography or with an endotoxin antagonist, B464, confirming that the contaminating pyrogen was endotoxin. A noteworthy finding was that one of the endotoxin-containing extracts showed very weak IL-6-inducibility in human monocytic cells in contrast to its high pyrogenicity to rabbits. The discrepancy could be explained based on differences between humans and rabbits in sensitivity to the endotoxin, because the extract showed higher proinflammatory-cytokine (TNF-alpha)-inducibility in rabbit whole-blood cells (WBCs) than human WBCs. The results suggest that the LAL test is a useful method of detecting endotoxin contamination in wound dressings and the MM6-CA8 assay is a good supplement to the LAL test for evaluating pyrogenicity in humans accurately.

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Quantitative determination of endotoxins on surgical gloves

Cited by 14 publications

References 7 publications

Chemical and biological evaluation of endotoxin contamination on natural rubber latex products

Chemical and biological evaluation of endotoxin contamination on natural rubber latex products

Endotoxins and pyrogens in the context of the use of protective gloves in hospital environment

Endotoxin contamination in wound dressings made of natural biomaterials

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