Quantitative Determination of Fluoxetine in Pharmaceuticals and Plasma Samples Using Bromatometric Method

Abstract

Asensitive and simple spectrophotometric method has been developed for quantitative determination of fluoxetine using bromatometric method. The method is based on the addition of measured excess amount of bromate-bromide mixture to fluoxetine in hydrochloric acid medium. The residual bromine was determined by reacting with a fixed amount ofmethyl orange and absorbance was measured at 505 nm. The amount of bromine reacted corresponds to the amount of fluoxetine. Linear relationship between absorbance and fluoxetine concentration was found and Beer's law was obeyed in the concentration range of 0.4-12 µg•mL-1. The molar absorptivity was found to be 3.8 × 10 4 L•mol-1 •cm-1. The limit of detection and limit of quantification was calculated and found to be 0.32 µg•mL-1 and 1.0 µg•mL-1 respectively. The common excipients were investigated for their interferences effect in the assay. The validity of the developed method was checked through recoveries studies and successfully applied to the determination of fluoxetine in bulk powder, pharmaceutical formulations and spiked human plasma samples. The percent recoveries were found to be in the range of 97.0%-101.0% for pharmaceutical formulations and from 97.0%-99.0% for spiked human plasma.

New Spectrofluorimetric Method for Determining Serotonin: Application to Human Urine

“…It has been shown that platelet serotonin is the most sensitive indole marker for the diagnostic of caracinoid tumors, especially those with low serotonin production (Meijer & al., 2000;Kema et al, 2001;Kema et al, 1992). There are many approaches to quantify levels of the 5-HT in human urine including voltammetry (Michael & Wightman, 1999;Baur, Kristensen, May, Wiedemann, & Wightman, 1988), capillary electrophoresis (C.E Lunte, Martin & S. M Lunte, 2000;Wallingford & Ewing, 1987), chemical luminescence (Marklova, Makovickova, & Krakorova, 2000), electron capture (CDE) or mass spectrometric (MS) detection (Milner, Hall, Davis, Brissie, & Robinson, 1998;Eap et al, 1998;Fontanille, Jourdil, Villier, & Bessard, 1997;Shah, Jan, Khan, & Durrani, 2012;Esrafili, Yamini, & Shariati, 2007), enzyme immunoassay, radio immunoassay (Chauveau, Fert, Morel, & Delagee, 1991;Engbaek & Voldby, 1982;Filik, Avan, & Aydar, 2014;Wei, et al, 2014;Zhu, Steiner, Munn, Daws, & Heewlett, 2007). All the recent assays were based either on high-performance liquid chromatography (HPLC) with fluorimetric or UV detection (Ravindra, 2014;Agrawal et al, 2013;Akerman, Jolkokonen, Huttunen, & Penttila, 1998), or on gas chromatography (GC) with nitrogen phosphorus detector (NPD).…”